Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,138,533 C→T G52G (GGC→GGT)  flgK → flagellar hook‑filament junction protein 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,138,5330CT96.3% 72.3 / ‑4.1 28G52G (GGC→GGT) flgKflagellar hook‑filament junction protein 1
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (14/12);  total (16/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01

AGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATA‑CTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGCTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGC  >  NC_000913/1138286‑1138772
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                
aGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGCTAATA‑ATCTGTAATTGAAGGCTTATCAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGCCTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATCACGGTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCCGGTTGGGCGCTGGCGGTTgg                                                                                                                                                                                                                                               >  2:152535/1‑251 (MQ=255)
aGTAAGAACAGTGATAATCTGTTATAAAAAACTCAAGTCCGGCGGTTCGCTGCAGA‑AATAACCCTGAAATTGAAGGATTATAAGGAACCTCCATGTCCAGCTTGATAAATAACGCCAGGAGGGGACGGAACGCGGCCAAGGCGGCGTTAAATCCGGCAAGTAATAATATCTCCAGCAATAACGTTGCCGGATTTCCCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTgg                                                                                                                                                                                                                                               <  2:240777/251‑1 (MQ=255)
     gactaTTGATACTCTGTTCTGAATAACTCAAGTCCGGCGTGTCTCTGCCGTTAATA‑CCCTTTAATTGAAGGCTTATAAGTACCCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTTAACGCGCCCCAGGCGGCTTTAAATACGGCAATTAATAATATCTCCAGCTATATCTTTGCCGGATATACCCGCCAAACCTCTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCggttgggttg                                                                                                                                                                                                                                           <  1:432666/246‑1 (MQ=255)
                     gtcttaaTAACTCAAGTCCGGCTGGGAGCTGACGATAATA‑CTCTGTAATTGAAGGCTTATAAGGAACACCCATGTCCAGCTTGATTAATATCGCCATGAGCGGATTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTAGAACGTTGCCTGATATACCCGCCAAACCTCTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGt                                                                                                                                                                                                                          <  1:483173/245‑1 (MQ=255)
                       cTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATA‑CTATGTAATTGAAGGCTTCTAAGAAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGGTGGCAAAGGGGTCT‑ACGtttt                                                                                                                                                                                                                       >  1:484128/1‑250 (MQ=255)
                              aCTCAAGGCCGGCGGGTCGCTGCCGATAATA‑CTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGtgtg                                                                                                                                                                                                                <  1:409498/251‑1 (MQ=255)
                                ccAAGTCCGGCGGGTCGCTGCCGATAATA‑CTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCa                                                                                                                                                                                                              <  1:209497/250‑1 (MQ=255)
                                        gacggGTCCCTGCCGAAAATA‑CTCTGTAATTGAAGGCTTATAAGGACCCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGTACTGAACGCGGCCCAGGCGGCGTTAATTACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGt                                                                                                                                                                                                      <  1:88439/249‑1 (MQ=255)
                                                                          aGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTTTTACCAACCAGTTACGTgcgg                                                                                                                                                                     >  1:435280/1‑251 (MQ=255)
                                                                          aGGCTTATAAGGAACCTCAAGGTCAAGCTGTATTAATAACGCCATGAGGGGACGGAAGGCGGCCCAGGGGGCGTTAAAGCGGGAAAGAAATAATATCCCCGGCGATAAGGGTGCCGGATATACCCGCCAAACCACTATTATGGCGCATGCCAATAGCACGTTGGGCGCTGGCGGTTGGGGTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTAGTACCAACCAGTTACGTacgg                                                                                                                                                                     <  2:173579/251‑1 (MQ=255)
                                                                                                                  gCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGCTATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGACATGGTGTCTCACTTTTTTGG‑GTGCAGGGCGAGTATGCTGCGTTTTTTACCAACCCGTTACGTTCGGCGCAGACCCAAAGTGTCGGGCTGACTGCCCGCTAttaca                                                                                                                              >  2:347103/1‑246 (MQ=255)
                                                                                                                                     cgGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCCATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGGTGGCAATGGGGTCT‑ACGTTTTTGGTTTGCAGCGTGGGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCCGACGCAAAGTAGCGGTCTGACTTCCCGCTATGAGCCGATGTCGCAAAtagccaat                                                                                                          >  1:49463/1‑244 (MQ=255)
                                                                                                                                              cggcgTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGGTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGGATGATGCGTTTATTAACAACCAGTTACGTGCGGCGCAGACGGCAAGTAGCGGTCTGACTGCCCGCTATTAGCAGATTTCGGAAATCGACAAAATGCTCTcc                                                                                                 >  1:302288/1‑251 (MQ=255)
                                                                                                                                                gcgTTAAATACTGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTccac                                                                                               <  1:215788/251‑1 (MQ=255)
                                                                                                                                                   ttAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGGAATGGGGTCT‑AAGTTTCTGGTGGGCCGCGTGAGTATGATGCGt                                                                                                                                                                                             >  2:408420/1‑154 (MQ=255)
                                                                                                                                                   ttAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGt                                                                                                                                                                                             <  1:408420/154‑1 (MQ=255)
                                                                                                                                                                  aataatATCTCCAGCTATACCGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGc                                                                             <  1:40694/251‑1 (MQ=255)
                                                                                                                                                                          ctcCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGGGTGCAGCGGGGGTATGATGCGGTTATTACCAACCCGTTACGTGCGGCGCCGACGCCAAGTAACGGTCTGACTGCCCGCTATGGGCAGATGTCGAAAAACGACAAAATGCTCTCCCCCAGTACCTCTTCGCTGGCAacacca                                                                      >  2:317611/1‑248 (MQ=255)
                                                                                                                                                                                  ataACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGa                                                              <  1:151438/250‑1 (MQ=255)
                                                                                                                                                                                         tGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGACGCTGGCGGTTGGGTTGGCAATGGGGTCT‑ACCTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGAGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGACGAAGTCGAAAATCGCCAATATGATCTCCACCAGTACCTCTTCGCTGGCAACCAAGATGCAGGACttcttcc                                                      >  1:199528/1‑250 (MQ=255)
                                                                                                                                                                                               atatACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTAGAACGCTGGCGGATAGGTTTGACCTGGTGTCG‑ACCTTTATAGAGTGCAGGGTGGGTATGATGAGTTTATTTACAACCAGTTAAGTGCGGCGCAGCCGCCAAGTAGGGGACTGACTGCCCGCTATGAGCAGATATCCAAAATCGTCACAATTCTCGCCAACACTACCTCTTCGCTGGCAACACAgttacaaggttttttccccacgc                                                >  2:103797/1‑229 (MQ=255)
                                                                                                                                                                                                    cccGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCCACCCAGATGCAGGATTTCTTCACCAGCCTGCa                                            >  1:393798/1‑250 (MQ=255)
                                                                                                                                                                                                               aCTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAAAGGTGTCT‑ACGTTTCTGGGGTGCAGCGTGAGTATGATGGGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGAATGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGGTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTTTTCACCCGCCTGCAAACGCTgggg                                  >  1:67034/1‑247 (MQ=255)
                                                                                                                                                                                                                 tattatGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGGATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACAACGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAgg                               >  1:327089/1‑249 (MQ=255)
                                                                                                                                                                                                                      tGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGGGTAAcgcg                         >  1:178138/1‑251 (MQ=255)
                                                                                                                                                                                                                       ggCGCAGGCCAATAGCACGTTGGGCGCTGGCGGCTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCgg                        >  1:16945/1‑251 (MQ=255)
                                                                                                                                                                                                                                    aGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAgc           <  1:278602/251‑1 (MQ=255)
                                                                                                                                                                                                                                         gTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGGCCCGGCAGCGCGcc      >  1:178991/1‑251 (MQ=255)
                                                                                                                                                                                                                                             ggCGCTGGCGGTTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGgc  <  1:65019/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                
AGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATA‑CTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGCTGGGTTGGCAATGGTGTCT‑ACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGC  >  NC_000913/1138286‑1138772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑