Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,127,338	G→A	G178D (GGC→GAC)	yceM →	putative oxidoreductase YceM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,127,338	0	G	A	100.0%	184.7 / NA	59	G178D (GGC→GAC)	yceM	putative oxidoreductase YceM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/23);  total (36/23)

GGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCT  >  NC_000913/1127126‑1127570                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         ggAACTGGGGGCGCGGAAAAAACTGGCGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACGCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAac                                                                                                                                                                                                     <  2:85067/250‑1 (MQ=255)                   aaaaCTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGt                                                                                                                                                                                  <  1:462151/251‑1 (MQ=255)                     aaCTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGttt                                                                                                                                                                                <  1:196945/251‑1 (MQ=255)                        tGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATg                                                                                                                                                                                                                         >  2:166675/1‑207 (MQ=255)                        tGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATg                                                                                                                                                                                                                         <  1:166675/207‑1 (MQ=255)                           cGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCg                                                                                                                                                                            >  1:215650/1‑249 (MQ=255)                                aTGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATCCAGCGCTGTGGTTGTCAAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGCATAACGAAGCTGGCGCAATGCTGTTTGCCGAGCAcc                                                                                                                                                                      >  1:472370/1‑250 (MQ=255)                                      ggTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTc                                                                                                                                                               >  1:354736/1‑251 (MQ=255)                                              ccGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCACTAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGATGTGGTTGTCGAGCGACAATGCCTCTCTGGGTGGCGGTACGCTACTGACTAACGACGCTGGAGAAATGCTGTTTGCCGAGCACCATTTTTAGGCTGGTc                                                                                                                                                       >  2:412101/1‑251 (MQ=255)                                              ccGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTc                                                                                                                                                       <  1:143872/251‑1 (MQ=255)                                               cgtcgtTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTcc                                                                                                                                                      >  2:435706/1‑251 (MQ=255)                                                  cgtTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCtt                                                                                                                                                    <  1:290756/250‑1 (MQ=255)                                                   gtTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTg                                                                                                                                                  <  2:96648/251‑1 (MQ=255)                                                    ttgcGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACCAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGc                                                                                                                                                 >  2:172105/4‑251 (MQ=255)                                                          cccacTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATca                                                                                                                                           <  1:435706/250‑1 (MQ=255)                                                            cacTCTACGGTGAGTTAACAACGCAACTCGCCCCCGCAGCCTCGCTAAGAATGGATAACCATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTAACTAACGACGCTGGCGACATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAgctcccc                                                                                                                                         >  2:460460/1‑245 (MQ=255)                                                              ctctACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATcaccac                                                                                                                                       >  2:386349/1‑251 (MQ=255)                                                                ctACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCt                                                                                                                                     >  1:101768/1‑251 (MQ=255)                                                                  aCGGTGAGTTAAAAACGCAACTCGCCATCGCAGCCTCGCTAAGAAGGGATAAACATCGTAGCAATAGCGTCGGGCCACCCGATCTTTATTTCACGTTGCTGGATGATTATCGGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCGGGTCCTTTGCAGATCACCACCTGt                                                                                                                                   <  2:349130/251‑1 (MQ=255)                                                                        aGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCAACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTATGCAGATCACCACCTGTATGCAt                                                                                                                             >  1:215278/1‑251 (MQ=255)                                                                             aaaaCGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCg                                                                                                                        >  1:410903/1‑251 (MQ=255)                                                                                        cGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTc                                                                                                             >  1:483697/1‑251 (MQ=255)                                                                                         gCCACCGCAGCCTCGCTAAGACTGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCGGAGCCCCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTc                                                                                                             >  2:273480/1‑250 (MQ=255)                                                                                            aCCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCg                                                                                                         >  2:83105/1‑251 (MQ=255)                                                                                                cAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGaa                                                                                                     <  2:465598/251‑1 (MQ=255)                                                                                                   ccTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATAACGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAAcc                                                                                                  >  1:267683/1‑251 (MQ=255)                                                                                                           aGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGc                                                                                          >  1:391458/1‑251 (MQ=255)                                                                                                             aaTGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCg                                                                                        >  2:480902/1‑251 (MQ=255)                                                                                                                    aaaCATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGtgactg                                                                                  >  1:40428/1‑250 (MQ=255)                                                                                                                          cGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTgc                                                                           >  2:103495/1‑251 (MQ=255)                                                                                                                           gTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGTATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTgcg                                                                          <  1:469957/251‑1 (MQ=255)                                                                                                                             aGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCt                                                                        >  2:182536/1‑251 (MQ=255)                                                                                                                               cAATAGCGTCGGGCCACTCGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCAGCGCCGTGCCGGAAGTCAGCGTGAATCCGTGCAGGCCGTGACTGACGGTGCGCTCa                                                                      <  1:198541/251‑1 (MQ=255)                                                                                                                                 aTAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACTCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATc                                                                    <  1:122274/251‑1 (MQ=255)                                                                                                                                      gTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGGCAGCGTGAAACCGTGCAGGCCGTGACCGACGGGTCGCTCATCGAcct                                                               >  2:118147/1‑249 (MQ=255)                                                                                                                                          ggCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGCCATTACg                                                           >  2:113711/1‑251 (MQ=255)                                                                                                                                             cacaCGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGat                                                        >  1:134974/1‑251 (MQ=255)                                                                                                                                                   aTCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCCCCACCTGTATGCATCGCCGTGCCGGAAGTCCGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCCCATCGACATTACGGGTATgcgc                                                  >  2:297903/1‑251 (MQ=255)                                                                                                                                                        tATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGGCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGTTATGCGCGAATg                                             >  2:460788/1‑251 (MQ=255)                                                                                                                                                         aTTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATgg                                            >  1:420929/1‑251 (MQ=255)                                                                                                                                                          tttCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGGGCAGGCCGTGACTGACGGTGCCCTCATCGACATTACGGGTATGCGCGAATggg                                           >  2:132704/1‑250 (MQ=255)                                                                                                                                                             cACGTTGCTGGATGATTAACTGCAGGGGGTGGATACGGCGCTGTGGGTGGCGAGCGACAAAGCCTCGCTGGAAGGCGGTACGATACTGACTAACGACGCTGGCGAAATGCTTTGTGCCGAGCACCATTGTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTg                                        <  2:267683/251‑1 (MQ=255)                                                                                                                                                                        attatTATCGGCATGTGGTGGATACCGCGGTGTGGTTGTCGAGCGAAAAAGCCTCTCGGGATGGCGGTACGCTACTGTCTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGGGAAACCGTGCAGGCCGTGACGGACGGTGCGCTCATCGACATTACGGAGATGCGCGAATGGCGTGAGGAGCGCggg                             <  2:206053/248‑1 (MQ=255)                                                                                                                                                                           atTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTTCCGGAAGTCAGCGTGAAACCGGGCAGGCCGTGACTGACGGTGCGCTCATCGACATTCCGGATATGCGCGAATGGCGTGAGGGGCGCGGGccg                          >  2:287545/1‑249 (MQ=255)                                                                                                                                                                           atTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGc                            >  1:87897/1‑249 (MQ=255)                                                                                                                                                                              aTCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGc                       >  2:64823/1‑251 (MQ=255)                                                                                                                                                                                 tGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGc                                           <  2:371934/228‑1 (MQ=255)                                                                                                                                                                                 tGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGc                                           >  1:371934/1‑228 (MQ=255)                                                                                                                                                                                  gCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATAACGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGAGGGCAGGGCGt                     >  1:274433/1‑249 (MQ=255)                                                                                                                                                                                   cATGCGGTGGATACCGCGCGGTGGTTGTCGAGCGACAAAGCCTCTCGGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGt                  <  2:284857/251‑1 (MQ=255)                                                                                                                                                                                         gtggATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCaaa             >  2:427358/1‑248 (MQ=255)                                                                                                                                                                                         gtggATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATaa            <  1:83105/251‑1 (MQ=255)                                                                                                                                                                                           ggATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCa                                                                                                            >  2:263790/1‑153 (MQ=255)                                                                                                                                                                                           ggATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCa                                                                                                            <  1:263790/153‑1 (MQ=255)                                                                                                                                                                                            gATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAAcc         <  1:30426/251‑1 (MQ=255)                                                                                                                                                                                              tACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACATGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCCGGGCGTTGTGCATAACCCg        >  2:481270/1‑250 (MQ=255)                                                                                                                                                                                               aCCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGAt      <  1:427358/251‑1 (MQ=255)                                                                                                                                                                                                 cgcgCTGTGGGTGTCGAGCGAAAAAGCCGCGCTGGATGGCGGTACGCTACTGACTAACGACGCGGGCGAAATGCTGTTTGCCGAGCACCATTTTGCGGCTGGTCCTATGCAGAGCACCCCCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGGGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTc    <  2:215278/251‑1 (MQ=255)                                                                                                                                                                                                   cgcTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCt  <  1:103495/251‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         GGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCT  >  NC_000913/1127126‑1127570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑