Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,127,334	G→A	G177S (GGC→AGC)	yceM →	putative oxidoreductase YceM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,127,334	0	G	A	100.0%	191.5 / NA	59	G177S (GGC→AGC)	yceM	putative oxidoreductase YceM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/23);  total (36/23)

CGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCT  >  NC_000913/1127089‑1127570                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             cGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTTCATGTGGTGGATACCGCGCTGTGGTTGTCGagcgac                                                                                                                                                                                                                                         <  1:147597/251‑3 (MQ=255)  gCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGagcgaca                                                                                                                                                                                                                                        <  1:386729/251‑4 (MQ=255)                                      ggAACTGGGGGCGCGGAAAAAACTGGCGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACGCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAac                                                                                                                                                                                                     <  2:85067/250‑1 (MQ=255)                                                        aaaaCTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGt                                                                                                                                                                                  <  1:462151/251‑1 (MQ=255)                                                          aaCTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGttt                                                                                                                                                                                <  1:196945/251‑1 (MQ=255)                                                             tGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATg                                                                                                                                                                                                                         <  1:166675/207‑1 (MQ=255)                                                             tGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATg                                                                                                                                                                                                                         >  2:166675/1‑207 (MQ=255)                                                                cGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCg                                                                                                                                                                            >  1:215650/1‑249 (MQ=255)                                                                     aTGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATCCAGCGCTGTGGTTGTCAAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGCATAACGAAGCTGGCGCAATGCTGTTTGCCGAGCAcc                                                                                                                                                                      >  1:472370/1‑250 (MQ=255)                                                                           ggTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTc                                                                                                                                                               >  1:354736/1‑251 (MQ=255)                                                                                   ccGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCACTAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGATGTGGTTGTCGAGCGACAATGCCTCTCTGGGTGGCGGTACGCTACTGACTAACGACGCTGGAGAAATGCTGTTTGCCGAGCACCATTTTTAGGCTGGTc                                                                                                                                                       >  2:412101/1‑251 (MQ=255)                                                                                   ccGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTc                                                                                                                                                       <  1:143872/251‑1 (MQ=255)                                                                                    cgtcgtTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTcc                                                                                                                                                      >  2:435706/1‑251 (MQ=255)                                                                                       cgtTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCtt                                                                                                                                                    <  1:290756/250‑1 (MQ=255)                                                                                        gtTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTg                                                                                                                                                  <  2:96648/251‑1 (MQ=255)                                                                                         ttgcGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACCAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGc                                                                                                                                                 >  2:172105/4‑251 (MQ=255)                                                                                               cccacTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATca                                                                                                                                           <  1:435706/250‑1 (MQ=255)                                                                                                 cacTCTACGGTGAGTTAACAACGCAACTCGCCCCCGCAGCCTCGCTAAGAATGGATAACCATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTAACTAACGACGCTGGCGACATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAgctcccc                                                                                                                                         >  2:460460/1‑245 (MQ=255)                                                                                                   ctctACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATcaccac                                                                                                                                       >  2:386349/1‑251 (MQ=255)                                                                                                     ctACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCt                                                                                                                                     >  1:101768/1‑251 (MQ=255)                                                                                                       aCGGTGAGTTAAAAACGCAACTCGCCATCGCAGCCTCGCTAAGAAGGGATAAACATCGTAGCAATAGCGTCGGGCCACCCGATCTTTATTTCACGTTGCTGGATGATTATCGGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCGGGTCCTTTGCAGATCACCACCTGt                                                                                                                                   <  2:349130/251‑1 (MQ=255)                                                                                                             aGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCAACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTATGCAGATCACCACCTGTATGCAt                                                                                                                             >  1:215278/1‑251 (MQ=255)                                                                                                                  aaaaCGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCg                                                                                                                        >  1:410903/1‑251 (MQ=255)                                                                                                                             cGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTc                                                                                                             >  1:483697/1‑251 (MQ=255)                                                                                                                              gCCACCGCAGCCTCGCTAAGACTGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCGGAGCCCCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTc                                                                                                             >  2:273480/1‑250 (MQ=255)                                                                                                                                 aCCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCg                                                                                                         >  2:83105/1‑251 (MQ=255)                                                                                                                                     cAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGaa                                                                                                     <  2:465598/251‑1 (MQ=255)                                                                                                                                        ccTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATAACGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAAcc                                                                                                  >  1:267683/1‑251 (MQ=255)                                                                                                                                                aGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGc                                                                                          >  1:391458/1‑251 (MQ=255)                                                                                                                                                  aaTGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCg                                                                                        >  2:480902/1‑251 (MQ=255)                                                                                                                                                         aaaCATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGtgactg                                                                                  >  1:40428/1‑250 (MQ=255)                                                                                                                                                               cGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTgc                                                                           >  2:103495/1‑251 (MQ=255)                                                                                                                                                                gTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGTATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTgcg                                                                          <  1:469957/251‑1 (MQ=255)                                                                                                                                                                  aGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCt                                                                        >  2:182536/1‑251 (MQ=255)                                                                                                                                                                    cAATAGCGTCGGGCCACTCGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCAGCGCCGTGCCGGAAGTCAGCGTGAATCCGTGCAGGCCGTGACTGACGGTGCGCTCa                                                                      <  1:198541/251‑1 (MQ=255)                                                                                                                                                                      aTAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACTCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATc                                                                    <  1:122274/251‑1 (MQ=255)                                                                                                                                                                           gTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGGCAGCGTGAAACCGTGCAGGCCGTGACCGACGGGTCGCTCATCGAcct                                                               >  2:118147/1‑249 (MQ=255)                                                                                                                                                                               ggCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGCCATTACg                                                           >  2:113711/1‑251 (MQ=255)                                                                                                                                                                                  cacaCGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGat                                                        >  1:134974/1‑251 (MQ=255)                                                                                                                                                                                        aTCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCCCCACCTGTATGCATCGCCGTGCCGGAAGTCCGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCCCATCGACATTACGGGTATgcgc                                                  >  2:297903/1‑251 (MQ=255)                                                                                                                                                                                             tATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGGCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGTTATGCGCGAATg                                             >  2:460788/1‑251 (MQ=255)                                                                                                                                                                                              aTTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATgg                                            >  1:420929/1‑251 (MQ=255)                                                                                                                                                                                               tttCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGGGCAGGCCGTGACTGACGGTGCCCTCATCGACATTACGGGTATGCGCGAATggg                                           >  2:132704/1‑250 (MQ=255)                                                                                                                                                                                                  cACGTTGCTGGATGATTAACTGCAGGGGGTGGATACGGCGCTGTGGGTGGCGAGCGACAAAGCCTCGCTGGAAGGCGGTACGATACTGACTAACGACGCTGGCGAAATGCTTTGTGCCGAGCACCATTGTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTg                                        <  2:267683/251‑1 (MQ=255)                                                                                                                                                                                                             attatTATCGGCATGTGGTGGATACCGCGGTGTGGTTGTCGAGCGAAAAAGCCTCTCGGGATGGCGGTACGCTACTGTCTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGGGAAACCGTGCAGGCCGTGACGGACGGTGCGCTCATCGACATTACGGAGATGCGCGAATGGCGTGAGGAGCGCggg                             <  2:206053/248‑1 (MQ=255)                                                                                                                                                                                                                atTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTTCCGGAAGTCAGCGTGAAACCGGGCAGGCCGTGACTGACGGTGCGCTCATCGACATTCCGGATATGCGCGAATGGCGTGAGGGGCGCGGGccg                          >  2:287545/1‑249 (MQ=255)                                                                                                                                                                                                                atTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGc                            >  1:87897/1‑249 (MQ=255)                                                                                                                                                                                                                   aTCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGc                       >  2:64823/1‑251 (MQ=255)                                                                                                                                                                                                                      tGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGc                                           <  2:371934/228‑1 (MQ=255)                                                                                                                                                                                                                      tGCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGc                                           >  1:371934/1‑228 (MQ=255)                                                                                                                                                                                                                       gCATGTGGTGGATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATAACGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGAGGGCAGGGCGt                     >  1:274433/1‑249 (MQ=255)                                                                                                                                                                                                                        cATGCGGTGGATACCGCGCGGTGGTTGTCGAGCGACAAAGCCTCTCGGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGt                  <  2:284857/251‑1 (MQ=255)                                                                                                                                                                                                                              gtggATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCaaa             >  2:427358/1‑248 (MQ=255)                                                                                                                                                                                                                              gtggATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATaa            <  1:83105/251‑1 (MQ=255)                                                                                                                                                                                                                                ggATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCa                                                                                                            >  2:263790/1‑153 (MQ=255)                                                                                                                                                                                                                                ggATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCa                                                                                                            <  1:263790/153‑1 (MQ=255)                                                                                                                                                                                                                                 gATACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAAcc         <  1:30426/251‑1 (MQ=255)                                                                                                                                                                                                                                   tACCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACATGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCCGGGCGTTGTGCATAACCCg        >  2:481270/1‑250 (MQ=255)                                                                                                                                                                                                                                    aCCGCGCTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGAt      <  1:427358/251‑1 (MQ=255)                                                                                                                                                                                                                                      cgcgCTGTGGGTGTCGAGCGAAAAAGCCGCGCTGGATGGCGGTACGCTACTGACTAACGACGCGGGCGAAATGCTGTTTGCCGAGCACCATTTTGCGGCTGGTCCTATGCAGAGCACCCCCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGGGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTc    <  2:215278/251‑1 (MQ=255)                                                                                                                                                                                                                                        cgcTGTGGTTGTCGAGCGACAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCt  <  1:103495/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             CGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCT  >  NC_000913/1127089‑1127570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑