Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,762	G→A	V282V (GTC→GTT)	pyrC ←	dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,762	0	G	A	100.0%	130.1 / NA	41	V282V (GTC→GTT)	pyrC	dihydroorotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/15);  total (26/15)

TTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGG  >  NC_000913/1121517‑1122007                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       ttatACAGGTAAATTAACCTAATGACAACAAGCAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGCTGAATGTGTCGTTGACCGGCAACCCATAGACCTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCCACGCATTCATTATTTCAAAAACGGt                                                                                                                                                                                                                                                   >  2:231433/1‑251 (MQ=255) ttatACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGt                                                                                                                                                                                                                                                   >  2:381053/1‑251 (MQ=255)  tataCAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGGGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGt                                                                                                                                                                                                                                                   <  2:173016/250‑1 (MQ=255)   ataCAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCAGTCATTTCTTCAAAAACGGTa                                                                                                                                                                                                                                                  <  2:41597/250‑1 (MQ=255)           aaaaTAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTg                                                                                                                                                                                                                                         >  2:147517/1‑251 (MQ=255)                              aagAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGt                                                                                                                                                                                                                      >  1:351470/3‑251 (MQ=255)                                                      aaCGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCCAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTGGGGGCGTTGAAGACGCCCGCGCAGCCGCaa                                                                                                                                                                                               >  2:35377/1‑250 (MQ=255)                                                      aaCGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAACAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCacc                                                                                                                                                                                              >  2:186658/1‑249 (MQ=255)                                                            ccAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGctct                                                                                                                                                                                        >  1:407221/1‑251 (MQ=255)                                                                   aCCGTTTCCCCGGCGAGGAATGGAACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGGTTACAGAACAGAATGGTTCAGAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGGAACTGCCCAGAGCGGATGGGGAGGTGAAGAATCCCGCGCAGCCGAAAATGCTCTCTTTGCg                                                                                                                                                                                 >  2:16343/1‑251 (MQ=255)                                                                             cGGCGAGGAATGGCACCAGCGAGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAACGTGCTGCAAAGCATTCATTTCTTCACAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGCCGTGc                                                                                                                                                                        >  2:448432/1‑250 (MQ=255)                                                                              ggCGAGGAATGGCACCATCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTCCCAGTTCGATGAATGTGTCGTTGTCCGTCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAACCTGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTTAAGCAGCCCGCGCCGCCGCAACTGCTCTCTTTGCGATGACGTTCAt                                                                                                                                                                      <  1:16343/251‑1 (MQ=255)                                                                                       tGGCAACAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAACCCGCGCAGCCGCAACTGCTCTCTTTGCGCTTACGTGCATGTGGCGCa                                                                                                                                                              >  2:453700/1‑250 (MQ=255)                                                                                                         aggcagggCGATGCTTTCAGCAACCGGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATa                                                                                                                                           <  2:11558/244‑1 (MQ=255)                                                                                                                        ttCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAAcc                                                                                                                              <  1:158691/249‑1 (MQ=255)                                                                                                                               aCCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGAc                                                                                                                     <  1:35377/251‑1 (MQ=255)                                                                                                                                    ttGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGtt                                                                                                                >  1:333853/1‑251 (MQ=255)                                                                                                                                                     gTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTgg                                                                                               >  1:77691/1‑251 (MQ=255)                                                                                                                                                         gATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGa                                                                                            >  2:123380/1‑250 (MQ=255)                                                                                                                                                          aTGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAat                                                                                          <  1:147517/251‑1 (MQ=255)                                                                                                                                                                       tGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAgg                                                                             >  2:244964/1‑251 (MQ=255)                                                                                                                                                                        gACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTgg                                                                                                                         >  2:401135/1‑206 (MQ=255)                                                                                                                                                                        gACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTGCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTgg                                                                                                                         <  1:401135/206‑1 (MQ=255)                                                                                                                                                                                           tGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGGGGATGGGTAGACA‑ATACAGGTg                                                        >  2:403476/1‑251 (MQ=255)                                                                                                                                                                                                gCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTTCGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGac                                                   <  1:143067/251‑1 (MQ=255)                                                                                                                                                                                                         agaacagaaTGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTCGACA‑ATACAGGTGCGGACGCCCGCCTc                                          >  2:321933/1‑251 (MQ=255)                                                                                                                                                                                                         agaacagaaTGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGcc                                            <  1:188871/249‑1 (MQ=255)                                                                                                                                                                                                         agaacagaaTGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑AAACAGGTGCGGACGCACGcc                                            >  2:188871/1‑249 (MQ=255)                                                                                                                                                                                                               gaaTGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGCCTCCAAcc                                     >  1:133063/1‑250 (MQ=255)                                                                                                                                                                                                                   gCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGAAGCACGCCTCCAACCAGCat                                >  2:472358/1‑251 (MQ=255)                                                                                                                                                                                                                    cTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGCCTCCAACCAGCat                                >  1:77335/1‑250 (MQ=255)                                                                                                                                                                                                                      tCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGCCTCCAACCAGCata                               <  1:341184/249‑1 (MQ=255)                                                                                                                                                                                                                        aaaGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTATCGTAACTGCCCATCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGATCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGGAGACA‑ATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGt                           <  1:407956/251‑1 (MQ=255)                                                                                                                                                                                                                         aattgctgcAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGtt                          <  1:392306/248‑1 (MQ=255)                                                                                                                                                                                                                                  caaGCATTCATTTCTTCAAAAACGATAGCGACACTGCCCAGCGAGGTTGGCGCGTTGACGCAGCCCGCGCAGCCACAACTGCACTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGTTTAAAACGGCTGGCGACCAGTTCAAGCAATGCCTGTTGGAGAATATTACGTTTGAGTATGGGAAG‑CAGATACAGGAGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTaac                 >  2:71997/2‑250 (MQ=255)                                                                                                                                                                                                                                  aaaGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTaa                  >  1:78795/1‑250 (MQ=255)                                                                                                                                                                                                                                   aaGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAAc                >  2:43704/1‑251 (MQ=255)                                                                                                                                                                                                                                    aGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACa               <  1:321933/251‑1 (MQ=255)                                                                                                                                                                                                                                     gCATTCATGTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGTAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGAAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGAGAATATTACGTTTGAGGATGGGAAGACA‑ATACAGATGCGGACGCAGACCTCCAACCAGCATCTGGTTGCGGTTAAACa               >  2:275802/1‑250 (MQ=255)                                                                                                                                                                                                                                           attTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGAtg        >  1:278954/1‑251 (MQ=255)                                                                                                                                                                                                                                                  cAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCgg  <  1:430570/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       TTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACA‑ATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGG  >  NC_000913/1121517‑1122007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑