Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,120,111	G→A	A159V (GCG→GTG)	solA ←	N‑methyl‑L‑tryptophan oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,120,111	0	G	A	96.0%	144.0 / NA	51	A159V (GCG→GTG)	solA	N‑methyl‑L‑tryptophan oxidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/24);  minor base T (0/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTG  >  NC_000913/1119866‑1120356                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ccccGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGt                                                                                                                                                                                                                                                  <  2:401149/249‑1 (MQ=255)     ggTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGCCAAGCAGCTCCGGGAGCAGGTATTTTACCCATGTTCACGCGCAGACAGTCACTTTTTTCGCCTGATACTACCCGTCAGCCGTTTAAAAGGTTACGCCAGCATCGTCATGACGCATTGCGGTGAACGGGCAGCTGAACGGTTGCGCACAGCCCGCTCCCTTCACCAGTTgg                                                                                                                                                                                                                                               >  2:155639/1‑250 (MQ=255)      gTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGAt                                                                                                                                                                                                                                             <  2:371243/251‑1 (MQ=255)               gggAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGtttt                                                                                                                                                                                                                                    >  1:426495/1‑251 (MQ=255)                               cGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCGGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTg                                                                                                                                                                                                                    <  2:451938/251‑1 (MQ=255)                                   ataGCGGCCATCAGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTgcgc                                                                                                                                                                                                                 >  1:361169/1‑250 (MQ=255)                                       cGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCaaaa                                                                                                                                                                                                             >  1:386398/1‑250 (MQ=255)                                                     aTACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCACGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAACCCGGAATCAGttt                                                                                                                                                                                              >  2:189475/1‑251 (MQ=255)                                                       aCCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTc                                                                                                                                                                                             >  1:378183/1‑250 (MQ=255)                                                         taGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGATACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCaa                                                                                                                                                                                           >  1:42247/2‑250 (MQ=255)                                                           ggCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCGGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATa                                                                                                                                                                                        <  2:58231/251‑1 (MQ=255)                                                                aTACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCg                                                                                                                                                                                   <  1:45723/251‑1 (MQ=255)                                                                    tttGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCACTAGTTACGCCATCATCGTCATGACGACTTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAACAACCGGAATCAGTTTCAAATAAGCCGATGt                                                                                                                                                                               >  2:449378/1‑251 (MQ=255)                                                                        cGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGtt                                                                                                                                                                           >  2:397389/1‑251 (MQ=255)                                                                            cGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTcc                                                                                                                                                                       <  1:242942/251‑1 (MQ=255)                                                                                 tGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGATTCAAATAAGCCGATGTAGTTGTCCGGGAc                                                                                                                                                                  >  1:440063/1‑251 (MQ=255)                                                                                         cAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTAttt                                                                                                                                                          <  1:363884/251‑1 (MQ=255)                                                                                               cGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGcc                                                                                                                                                    >  1:87090/1‑251 (MQ=255)                                                                                                                         ccGCTCAGACAATCGCTTTTTTCGCCTGATTCTCCCCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCg                                                                                                                          <  2:270172/251‑1 (MQ=255)                                                                                                                                  cactCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTc                                                                                                                  >  1:284510/4‑250 (MQ=255)                                                                                                                                  cAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGAAGAATTGCGGTGACCGGGCAGTTGAAAAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGACAGTTAGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGCTGTAGTTGTCCGGACCGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGAATCGCGCTTTTc                                                                                                                  >  1:284516/1‑250 (MQ=255)                                                                                                                                       gCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGtt                                                                                                            >  1:366741/1‑251 (MQ=255)                                                                                                                                            tttCGCCTTATACTCCCCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGt                                                                                                        <  2:386398/250‑1 (MQ=255)                                                                                                                                            tttCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGt                                                                                                        <  1:201236/250‑1 (MQ=255)                                                                                                                                               cGCCTGATACCCCCCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGc                                                                                                     <  2:246849/250‑1 (MQ=255)                                                                                                                                                gCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGAATCGAGCTTTTCAACGTTGAGTTGcc                                                                                                    >  2:332567/1‑250 (MQ=255)                                                                                                                                                    gATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGGCGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTGCACCAGTTGGATCCAGGTTTTAATCGCCATTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCGTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTg                                                                                                <  2:304624/250‑1 (MQ=255)                                                                                                                                                    gATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGt                                                                                               >  2:82828/1‑251 (MQ=255)                                                                                                                                                       aCTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCg                                                                                            <  1:51414/251‑1 (MQ=255)                                                                                                                                                                cAGCCGTTTCAATAGTTACGCCATCAACGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTgg                                                                                   <  2:337560/251‑1 (MQ=255)                                                                                                                                                                 aGCCGTTTCAATAGTTACGCCATCAACGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTggg                                                                                  <  2:87090/251‑1 (MQ=255)                                                                                                                                                                      tttCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGtt                                                                                                       >  1:7734/1‑225 (MQ=255)                                                                                                                                                                      tttCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGtt                                                                                                       <  2:7734/225‑1 (MQ=255)                                                                                                                                                                        tCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGt                                                                            <  2:60571/250‑1 (MQ=255)                                                                                                                                                                           aTAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCCACGTTGAGTTGCCa                                                                                                   >  2:287390/1‑224 (MQ=255)                                                                                                                                                                           aTAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCa                                                                                                   <  1:287390/224‑1 (MQ=255)                                                                                                                                                                              gTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCgag                                                                     <  2:310671/251‑1 (MQ=255)                                                                                                                                                                                     catcatCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAAtgtg                                                              <  1:43863/251‑1 (MQ=255)                                                                                                                                                                                           cGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGGTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATc                                                         >  2:462326/1‑250 (MQ=255)                                                                                                                                                                                                     aaTTGCGGGGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCa                                               >  1:138170/1‑250 (MQ=255)                                                                                                                                                                                                         gCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATTTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGtt                                          <  1:397824/251‑1 (MQ=255)                                                                                                                                                                                                                  gggCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGAAAAAAAACGGAATCAGTTTAAACTAAGGCGATGTAGTTGTCAGGGACGCGTATTTCCGGCCAGCGGGACATAATCACTTGCGCATCGAGCTTTTCAACGTTGGGTTGAAATTGTTAGGCGATGTGGGCGACGTTGGCGAGAATTGAGGAATCAGCCGGGGCAAGGTTAATGACgac                                 >  1:461932/1‑249 (MQ=255)                                                                                                                                                                                                                  gggCAGTGGAACAGTTGCGCACAGCCCGCTTCCTTCTCCAGTTGGATCCAGTTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGGTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTGCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGTCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGATAATgccgcc                                 <  2:306856/251‑1 (MQ=255)                                                                                                                                                                                                                   ggCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCa                                <  2:116671/251‑1 (MQ=255)                                                                                                                                                                                                                     caggtGAACAGTTGCGCACATCCCGCTTCCTTCTCCCGTGGGATCCAGGTTTTAATGGCCAGTTCGCTGCGCAAAAAACCTGAATCAGTTTCAAATGAGCCGATGTAGTTGTGCGGGACGCGTAGTTCCGGCCAGCGGGCCATAAGCCCTTGCGCATCGAGCTTGTCATCGTTGAGTTGCCATTGTTCGGCGCTGGGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCaga                              <  1:448220/247‑1 (MQ=255)                                                                                                                                                                                                                         tGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAACAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCgg                          >  2:106317/1‑250 (MQ=255)                                                                                                                                                                                                                           aaCAGTTGCGCACCGCCCGCTCCCTTCACCCGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGTAAGCAGTGTCAAATAAGCCGATGTAGTTGTCCTGGACGCGTATTGCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCTCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTAc                        <  2:440403/251‑1 (MQ=255)                                                                                                                                                                                                                                     cactgCCCGCTTCCTTCCCCAGTTGAATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGATTCAAAAAAGCCGATGTAGTTGTCCGGGACGCGAATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGGGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTATTGACGcccgaccttacaacatagggt              >  2:441050/5‑232 (MQ=255)                                                                                                                                                                                                                                       caGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGAtc            <  1:397389/251‑1 (MQ=255)                                                                                                                                                                                                                                              cttccttcACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCg     >  2:196775/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ccttcACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTtg  >  2:376542/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTG  >  NC_000913/1119866‑1120356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑