Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,115,185	C→T	G102G (GGG→GGA)	mdtG ←	efflux pump MdtG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,115,185	0	C	T	96.9%	88.4 / ‑4.2	32	G102G (GGG→GGA)	mdtG	efflux pump MdtG
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (12/19);  total (12/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

ACACTGGCGGTAATAAAGAATACCGGACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGCCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGA  >  NC_000913/1114949‑1115384                                                                                                                                                                                                                                             |                                                                                                                                                                                                        acacTGGCGGTAATAAAGAATACCGGACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCACCGCACCACTCACGCCGCCTGTGGGGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGGACATGTGTGGCGGTAAGAGCATTAGCGTTGGGGACAAATCCGCCAAATAACCCAAGAAGCGCCCGCAGGAACAAAAACAGCCCGATATTTTTGGCCAGTCCCCTCAACACCAt                                                                                                                                                                                           >  1:229375/1‑251 (MQ=255)  cacTGGCGGTAATAAAGAATACCGGACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATc                                                                                                                                                                                          <  1:82759/251‑1 (MQ=255)           aaaaaaaGAATACGGGACGTAAGCCGTAGCTAGCGGCGAGCGGGCCTCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGccc                                                                                                                                                                                 <  2:78813/247‑1 (MQ=255)            aataaAGAATCCCGGACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACAACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGCCCCATCAACACCATCACGATGCCCa                                                                                                                                                                                <  1:24375/251‑1 (MQ=255)             ataaAGACTACCGGACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGCGCGAACCCCGCCCCCAGCCGCTTTTATTACGCGGTCCTTGTGTGGCGATAAGAGCCTTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGCTCAAAAACTGCCAGATATTTTTTGCCAGTCCCATCCACACCATCACGAAGCCCAt                                                                                                                                                                               >  2:39646/1‑251 (MQ=255)                    aTACCGGACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAgg                                                                                                                                                                        >  1:43268/1‑251 (MQ=255)                                    gTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCAAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATAACGATGCCCATGCCGAGGGCAGGGCGTAAttgc                                                                                                                                                         >  2:413347/1‑247 (MQ=255)                                      aGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGCGGGCAGCGCGTAAAAGCATg                                                                                                                                                      >  2:182891/1‑251 (MQ=255)                                                 aGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGcc                                                                                                                                           >  1:2721/1‑251 (MQ=255)                                                     gcccgccAGCCATGGGGCCGAGCACAGCACCACTAACGCCGCCTGTGGAGAGCGTCCCCAGCGCCCAGGCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGAGATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATTCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTa                                                                                                                                       <  2:475779/249‑1 (MQ=255)                                                        cgcagtccatgtgggcGAGCAACGTCCCTCTAAGGCCGCCTGTGGAGAGCGTCCCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACTCCTTCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACgg                                                                                                                                    <  2:149397/236‑1 (MQ=255)                                                          ccagccaTGGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTc                                                                                                                                  <  2:357564/251‑1 (MQ=255)                                                           cagccaTTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCTGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCg                                                                                                                                 <  1:355079/251‑1 (MQ=255)                                                                        gAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCAcccc                                                                                                                    <  1:39646/251‑1 (MQ=255)                                                                           cAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCaaa                                                                                                                 <  2:2721/251‑1 (MQ=255)                                                                                           ccgccTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGc                                                                                                 <  1:399116/251‑1 (MQ=255)                                                                                            cgccTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGcc                                                                                                <  1:400177/251‑1 (MQ=255)                                                                                               cTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCAt                                                                                                                                                       >  1:288198/1‑193 (MQ=255)                                                                                               cTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCAt                                                                                                                                                       <  2:288198/193‑1 (MQ=255)                                                                                                    gagaGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTGTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATaa                                                                                        <  2:43268/251‑1 (MQ=255)                                                                                                            aCCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTaa                                                                                <  1:208736/251‑1 (MQ=255)                                                                                                                   gcCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGCTGGCCGAAAATac                                                                                        >  1:399594/1‑235 (MQ=255)                                                                                                                   gcCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATaa                                                                                        <  2:399594/236‑1 (MQ=255)                                                                                                                                              tgtgtgGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAAGGTAATGCTGAAGACAATACCGGACCACATAGTCAgg                                                <  1:398482/249‑1 (MQ=255)                                                                                                                                                              cATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACg                              >  1:101604/1‑251 (MQ=255)                                                                                                                                                                       tGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAACTGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAgctgc                     >  2:434505/1‑251 (MQ=255)                                                                                                                                                                        ggggACAAATCCGCAAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGACAATAAAAATGTACTGCTGAAGACAATACCGGACCCCATATTCAGGGCGGAGTGACCGGTAACGCCAAgctgct                    >  2:302880/1‑251 (MQ=255)                                                                                                                                                                         gggACAAATCCGCCAAGTAACCCAATAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTc                   <  1:223731/251‑1 (MQ=255)                                                                                                                                                                          gggcAAATACGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACAGCAAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGCGGGCAGAGCGTAATAGCATGAGCTTTCGGCCTTTACGGTCGGCGAGTCCACCACAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGAAAATACCGGACCACATATTCAGGGCGGAGTGACCGGTCAAGCAAAgctgatc                   >  2:347754/4‑247 (MQ=255)                                                                                                                                                                                  ccGCCAAGGAACCCAAGTAGCGCCCGCACGATCAAAACCTGCCATATATCTTGTGACAGTCCCATCAACACCTTCCCGAGGCCCATGCCGAGGGCTGAGCGTAATAGCATGAGTTTTCGGCCTTTGCGGTCGGCGAGACCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAAGACCGGACCGCATATTCAGGGGGGAGTGTCCGGTATCGCCAATCTGCTAAACGTagag          <  2:340784/251‑1 (MQ=255)                                                                                                                                                                                   cGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAgg         <  2:120758/251‑1 (MQ=255)                                                                                                                                                                                           aaCCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTaaga  <  1:384883/250‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                        ACACTGGCGGTAATAAAGAATACCGGACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGCCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGA  >  NC_000913/1114949‑1115384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑