Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,109,740 C→T L136F (CTT→TTT)  opgG → osmoregulated periplasmic glucans biosynthesis protein G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,109,7400CT90.5% 56.1 / NA 21L136F (CTT→TTT) opgGosmoregulated periplasmic glucans biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/10);  minor base G (0/2);  total (9/12)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAG  >  NC_000913/1109516‑1109980
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
agATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTATAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGTTaa                                                                                                                                                                                                                         >  2:118359/1‑250 (MQ=255)
         ttAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGa                                                                                                                                                                                                                >  1:25574/1‑250 (MQ=255)
             tCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTGAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCg                                                                                                                                                                                                           >  1:468679/1‑251 (MQ=255)
                  aCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGc                                                                                                                                                                                                      >  2:385224/1‑251 (MQ=255)
                                                cATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGAtt                                                                                                                                                                        >  1:214893/1‑251 (MQ=255)
                                                   gcAAACTCGAGTGCTACCATCATGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGGCAAACGAATCAAATACAGCCCGGATTATTTCACTTGCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATATAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTgg                                                                                                                                                                      <  1:62316/249‑1 (MQ=255)
                                                    caaaccctaGTTCTACCATCAGGGTATGTACTTCGAGACCCCCGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCACGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGAAAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTg                                                                                                                                                                    <  2:130491/243‑1 (MQ=255)
                                                       acccGAGTTCTACCATCAGGGTATGTACCTCGAAACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGcag                                                                                                                                                                 <  2:368591/248‑1 (MQ=255)
                                                                              tGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGcc                                                                                                                                          >  2:9516/1‑251 (MQ=255)
                                                                                       aTACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCAGGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTg                                                                                                                                 <  2:347895/251‑1 (MQ=255)
                                                                                              ggTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTg                                                                                                                          >  1:78462/1‑251 (MQ=255)
                                                                                                  aaaaTAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAATGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGAGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGAtcc                                                                                                                      >  2:12441/1‑249 (MQ=255)
                                                                                                         aggAAGGGACTGCCACCGGCGTCATATGAATCAAAATCAGCGTGGATTATTTCACTTTCGGCGATGTCTAGCATGACAAAGATACGTTAAAAGACCTTGGCTTGGCCGGTTTTAAAGTGTTTTACCCGATCAACAGTAAATATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGTAGGTCAGGTTTATTGCCTTTCTGCCCGCGGCCTGGCATTTGATACCGCCTTg                                                                                                               <  2:438484/249‑1 (MQ=255)
                                                                                                                gaatGCCACCGCATTCAAACGAATTAAATTCAGCCCGGATTATTTCACTTTCGGCGATGGTCAGCATGAAAAAGAGACGGTAAAAGACCGTGGCTTGGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCg                                                                                                         <  2:352265/247‑1 (MQ=255)
                                                                                                                    gCCACCGCAGTCAAACGAAACAAATACAGCCCGGATTATTTCACTTGCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCGTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAATATAAAAACGATGAAATCGTCAGCATTCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGt                                                                                                      <  1:450039/249‑1 (MQ=255)
                                                                                                                                        cAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAag                                                                                <  1:385224/251‑1 (MQ=255)
                                                                                                                                                         attTCTCTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCTATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTTGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCg                                                                <  2:436905/250‑1 (MQ=255)
                                                                                                                                                                 ttCGGCGAGGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGGTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAAc                                                        <  2:25574/250‑1 (MQ=255)
                                                                                                                                                                                                    aGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTg                    >  1:240751/1‑251 (MQ=255)
                                                                                                                                                                                                                       ttttaaGTGGTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGGCAGCATGCTCGGGGCCAGCTATTTCCGCGTGAGTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAg  <  2:468679/246‑1 (MQ=255)
                                                                                                                                                                                                                       tttAAAGTGTTTTACCCAATCAACAGCAAAGAAAAAAACGATGAAATCGGTAGCATGCCCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGACAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTTGATCGATCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAg  <  1:404592/250‑1 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
AGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAG  >  NC_000913/1109516‑1109980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: