Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,090,508	C→T	E594E (GAG→GAA)	pgaA ←	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,090,508	0	C	T	100.0%	100.6 / NA	32	E594E (GAG→GAA)	pgaA	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/9);  total (23/9)

AAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCAAGATT  >  NC_000913/1090280‑1090744                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               aaaaTCAGTGAAAGCCCAGGAGACCCCGTACTTACTCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAc                                                                                                                                                                                                                          <  2:365744/251‑1 (MQ=255)    aTCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTg                                                                                                                                                                                                                      >  1:345406/1‑251 (MQ=255)      cAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTGTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑c                                                                                                                                                                                                                   <  2:379731/251‑1 (MQ=255)                   ggAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGCGCCAGATATTAC‑GTGA‑CCGCCACTCAaca                                                                                                                                                                                                       >  1:230404/1‑250 (MQ=255)                                ttACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACGAA‑TAAGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTc                                                                                                                                                                                         >  1:157386/1‑251 (MQ=255)                                          cATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATc                                                                                                                                                                               >  2:49634/1‑251 (MQ=255)                                           aTTTTGATACCAGCGAAAATAAGCATGAGCAATGTTGCCTGTAACACCATTTTTAATTGCCCGCAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTGACATTAACATCATACCAGCCAGCCAGGCGCGCGCCGGGTTTATGCTCATGATGGAAACCACGTTCAGCCGCTTCTTCCTCGCGCCAGATATTAC‑G‑GAGACGCCACTCAACCCCCACAAGCCAGTCGCGCACaaacc                                                                                                                                                                              >  2:482069/1‑248 (MQ=255)                                                           aCATAAGCCTGACCACTTTTGCTTGTAACACTTTTTTTCATTGCCGGTAATGGAACGCGGTGAGAGGGGCGTTCCATTTGC‑AACCAAATACGCCATTTATCATTAAAATCATACCAGCCAGCCAGGCGCGCGCCGGGTTTATGCCCATGATTGAAACCGCTTTCGGCGTATTCTCCCCCGAGCCAGATATTAC‑GGGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTaaa                                                                                                                                                              <  1:260972/251‑1 (MQ=255)                                                                  ccTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGC‑ACGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAACTTGTccc                                                                                                                                                       >  2:468800/1‑250 (MQ=255)                                                                  ccTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGAGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAAAAAGCCCTTTTCCTTCGCTAAATTgaccc                                                                                                                                                       >  1:84488/1‑247 (MQ=255)                                                                              tGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCg                                                                                                                                           >  1:26446/1‑251 (MQ=255)                                                                                ccTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCCCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGa                                                                                                                                          >  1:454798/1‑250 (MQ=255)                                                                                           aTTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAAc                                                                                                                              >  1:427800/1‑251 (MQ=255)                                                                                                  aTTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTAttct                                                                                                                                                                                                                                             <  2:84555/135‑1 (MQ=255)                                                                                                  aTTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTAttct                                                                                                                                                                                                                                             >  1:84555/1‑135 (MQ=255)                                                                                                     ccccGTAAGGGAACGGGGGGAGAGGGGCTTTCCCGTGGCGAACAAA‑TCCGCAAGTTATATTTAAAATCATCCCAGCCAGACGGGCGCGCGCCGGGTTTATGTTCATGATTAAAAACGCTTTCAGCGTATTCTGCCTCGAGCCAGATTTTCC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGtt                                                                                                                    <  1:361990/250‑1 (MQ=255)                                                                                                                  cgcgGAGAGAGGGGCTTTCATGTTGCTAACCAA‑TCCGCAAGTTATAATAAAAATCATACCACCAAGACAGGCGCGCGCCGGGTTTATGCTCATGATTAAAACCGCGTTCAGCTTATCCGGCCCCGAGCCAGATTTTCC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCggt                                                                                                       <  1:374721/251‑1 (MQ=255)                                                                                                                                   tCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATcc                                                                                                                                                                              >  2:375866/1‑163 (MQ=255)                                                                                                                                       gTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGtt                                                                                  >  1:88682/1‑251 (MQ=255)                                                                                                                                        ttGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGGGGtt                                                                                  >  1:401943/1‑250 (MQ=255)                                                                                                                                                cAA‑TACGCCAGTTATCATTAAAATCATACAAGCCAGCCAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCCGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGCATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTACATAAACAAAggtggtgccaccgacc                                                                         >  2:70340/1‑241 (MQ=255)                                                                                                                                                          aGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGttt                                                                 >  1:464988/1‑250 (MQ=255)                                                                                                                                                            ttATCATAAAAATCATCCCAGCCAGACAGGCGCGCGCGGGGTTTATGCTCATGATTAAAACCGCGTTCAGCTTATTCTCCCCCGACCAAAATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTAc                                                               <  1:255856/250‑1 (MQ=255)                                                                                                                                                                cATTAAAATCAACCCACCCAGCCGGGCGCGCGCTGGGTTTATGCTCATGATTGAAACCGCGTCCAGCGTATTCTCCCTCGAGCAAGATTTTAC‑GGGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACt                                                           <  1:340492/250‑1 (MQ=255)                                                                                                                                                                         cATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCt                                                 >  2:373601/1‑251 (MQ=255)                                                                                                                                                                                    gacgggcccgcgcggtgTTTATGCTCATAATAAAAACCCCGTACAGCTTATTCTGCCTGGAGCAATAT‑TTACCGTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAAt                                      <  2:26446/235‑1 (MQ=255)                                                                                                                                                                                                 cGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGGGAATAAACGATGGTGGTTAAGTCAACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGcc                          >  2:471861/1‑250 (MQ=255)                                                                                                                                                                                                  gggTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGcc                          >  1:352358/1‑249 (MQ=255)                                                                                                                                                                                                      ttATGCTCATGATTGAAAACGAGTTAAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAAAACCCGCAAGCCCGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGACTCCAGCAAAACCGCGCCAGTTATCCTTCAACGGTGGTGAATAAACGATGGTGGTTACGTCGACATCATGTTTACCACATTCCGGACCTTCGGCATCAATTCCTGTTGAGCCAGccg                     >  2:209272/1‑248 (MQ=255)                                                                                                                                                                                                           cTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTTGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGCTACGa                >  1:323088/1‑250 (MQ=255)                                                                                                                                                                                                              aTGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTa                                                                                                                                                                >  1:287635/1‑103 (MQ=255)                                                                                                                                                                                                               gtataaaaaaCGCTTTCGGCGTATTCTGCCTCGAACCAGATATTCC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGctc           <  1:407271/245‑1 (MQ=255)                                                                                                                                                                                                                gATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGctct          >  1:41253/1‑251 (MQ=255)                                                                                                                                                                                                                        aCGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTAC‑GTGA‑TCGCCACTCAACACCTGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGATAAATTGACCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCACGAtt  >  2:126934/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               AAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAA‑TACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTAC‑GTGA‑CCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCAAGATT  >  NC_000913/1090280‑1090744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑