Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,469	C→T	A711A (GCC→GCT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,469	0	C	T	95.0%	111.0 / ‑2.7	40	A711A (GCC→GCT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (25/13);  total (26/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

GTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTG  >  NC_000913/2229‑2706                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               gTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGAAGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGTAATGGGTTATAAAGAACCGGATCAGCGAGCTGGTCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGAGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCACGCAGAGGTTCACGCTGAGGGTGATg                                                                                                                                                                                                                                      >  1:41403/1‑251 (MQ=255)      ccGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGTGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGGCGTGAACTGGAGCTGGCGGATATTGTATTTGAACCTGTGCTGCCCGCTGAGCGTAACGCTGAGGGAGATGttccc                                                                                                                                                                                                                                 >  1:149127/1‑248 (MQ=255)            ttctttcaGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGACATGGGATATACCGAACCGGATCCGAGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGATCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGGGTTTAACGCTGAGGGTGATGTTGCCGCtttt                                                                                                                                                                                                                           >  1:299470/1‑251 (MQ=255)                ttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGTGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTTTgg                                                                                                                                                                                                                       >  2:81287/1‑251 (MQ=255)                ttcaggTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGGGGGTGATGTTGCCGCTTTTATgg                                                                                                                                                                                                                       >  1:65317/5‑251 (MQ=255)                  caggTTCGTTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGTAAAGGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTTGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTGTATGG‑c                                                                                                                                                                                                                     <  1:292100/248‑1 (MQ=255)                  caggTTCGCTTTCTTATATCTTCGGCAAGTTAGACGCAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGGAATTGAACCTGTGCTGCCCGCCGTGTTTAACGATGAGGGTGATGTTGCCGCTTTTATGG‑Cg                                                                                                                                                                                                                    >  2:4609/3‑251 (MQ=255)                        cGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGTAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTg                                                                                                                                                                                                              >  1:118446/1‑251 (MQ=255)                             tCTTATATCTTCGGCAAGTTAGACGCAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGCACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGGGATGTTGCCGCTTTTATGG‑CGAATCTGTcac                                                                                                                                                                                                          >  2:138510/1‑250 (MQ=255)                                tataTCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTcacag                                                                                                                                                                                                        <  1:231222/249‑2 (MQ=255)                                                 aGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGGTCGACGCTCTCTTTGCcg                                                                                                                                                                                     >  1:14192/1‑251 (MQ=255)                                                     gAAGGCATGAGTTTCTCCGAGGCGCCTACTCTGGCGCGGGAAATGGGTTATACCGTACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGCCGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCCGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTTTGGGCGA‑TATGTCACACCTCGACGGTGTCTTTTCcgcgtg                                                                                                                                                                                 >  2:119672/1‑249 (MQ=255)                                                     gAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGGTTAACGCTGAGGGTGATGGTGCCGCTTTTATGG‑CGACTCTGTCAAAGCTCGACGCTTTCTTTGCcgggcg                                                                                                                                                                                 >  2:102658/1‑251 (MQ=255)                                                       aGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGCATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGGTTAACGCTGAGGGTGATGTTTCCGCTTTTATGG‑CGAATCTGTACCAGCTCGACGATCTCTTTTCcgcgcgcg                                                                                                                                                                               >  2:478664/1‑251 (MQ=255)                                                        ggCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGTAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGt                                                                                                                                                                              <  2:118446/251‑1 (MQ=255)                                                                                actcTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGATATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGaa                                                                                                                                                       <  1:65562/247‑1 (MQ=255)                                                                                                ggggTTATACCGAACCGGATCCGCGAGATGATCTTGCTGGTATGGATGTAGCGCGTAAGCTATGGATTCTCGCTCGGGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGtt                                                                                                                                      <  2:233294/250‑1 (MQ=255)                                                                                                  ggTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTgg                                                                                                                                    >  1:196526/1‑251 (MQ=255)                                                                                                      ataacGAACCGGAGCCGCGAGAGGAGCTTGCTGGTATGGATGTAGCGCGTAAGCTATTGATTCGCGCTCGGGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAAGCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAat                                                                                                                                <  2:463220/247‑1 (MQ=255)                                                                                                          cGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCAACGCTCGACGATCTCCTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAGGGAAAAATTTTGCGCTATGTTGGCAATATtg                                                                                                                            >  2:425431/1‑251 (MQ=255)                                                                                                                    ccGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGAGTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAAGTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATg                                                                                                                   <  1:155343/250‑1 (MQ=255)                                                                                                                       cgAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGt                                                                                                                                                                              <  1:159745/188‑1 (MQ=255)                                                                                                                                     tGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAg                                                                                                  <  2:313393/250‑1 (MQ=255)                                                                                                                                        tATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAAACTGAGATGCACGCAGAGTTTAACGATGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGATCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTTATGAAGATGGGGCCTGCTGCGTggagcttg                                                                                              >  1:438581/1‑244 (MQ=255)                                                                                                                                               gtagcccggaagcTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGTTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAg                                                                                        <  2:14192/238‑1 (MQ=255)                                                                                                                                                   cgcgTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTGTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCTCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTAGGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGAt                                                                                                <  1:172928/238‑1 (MQ=255)                                                                                                                                                              ttGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGGGGATGGTAATGATcc                                                                        >  1:159382/1‑251 (MQ=255)                                                                                                                                                               tGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCg                                                                       >  1:347345/1‑251 (MQ=255)                                                                                                                                                                        ctcgTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCCCAGCTCGACGGTCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGATATGTTGGCAATATTGATGAAGATGGTGCCTTCCGCGTGTAGCTTGCCGCAGGTGATGGTAATGGTCTGCTGGTCaa                                                               >  2:20694/1‑250 (MQ=255)                                                                                                                                                                            tGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGAGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAACGTTTTTCGCTATGTTGGCAATATTGGTGAAGATGGGGCCTGCCGCTGGCAGATTGACGGAGTGGATGGTAATGAGCCGGTGTTCAAAGTg                                                           >  2:276528/1‑250 (MQ=255)                                                                                                                                                                                           aCTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGGAGGCCCGTGATGAAGGGAAAGGTTTGCGCTATGTTGGAAATATTGATGAAGATGGGGCCTGCCGCGGGAAGATTGCCGAGGTGGGTGGTAATGATCCGCTGTGCAAAGGGAAAAATGGCGaaaa                                             >  2:477064/1‑249 (MQ=255)                                                                                                                                                                                                 gCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTgg                                     >  1:145945/1‑251 (MQ=255)                                                                                                                                                                                                           attgaaattgaaCCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGAGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTtata                            >  1:458114/1‑250 (MQ=255)                                                                                                                                                                                                              gaaattgaaCCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACAATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGGAAAATAGCGAAAACGCCCTGGCCTTTTATAGcc                         >  2:98962/1‑250 (MQ=255)                                                                                                                                                                                                                   tgaaCCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGTCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAATACGCCCTGGCCTTTGATAGCCACtat                    <  2:435540/250‑1 (MQ=255)                                                                                                                                                                                                                    gaaCCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACtatta                  <  1:138510/251‑1 (MQ=255)                                                                                                                                                                                                                           tgctgcCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAACGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCCGccc           >  2:50597/1‑250 (MQ=255)                                                                                                                                                                                                                               gcCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAGGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATAGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTg        <  1:81287/250‑1 (MQ=255)                                                                                                                                                                                                                                    cAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGGTTGCCGAAGTGGATGGTAATGATCCGCTGTTCCAAGGGAAAAATGGCGCAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCACTGCCgatg  >  2:350808/1‑248 (MQ=255)                                                                                                                                                                                                                                    cAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGttt  >  2:82307/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               GTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGG‑CGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTG  >  NC_000913/2229‑2706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑