Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,086,153 C→T G123R (GGG→AGG)  pgaD ← poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,086,1530CT95.0% 50.4 / ‑3.6 21G123R (GGG→AGG) pgaDpoly‑N‑acetyl‑D‑glucosamine synthase subunit PgaD
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (8/11);  total (8/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

TGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGTCA  >  NC_000913/1085917‑1086398
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     
tGCCTCGTGGCGTGCGCTCAGGAATAAGTGACGCGGTGGAACGGTTCGAAGAAGATGAAATGGTTGGGATAGTCCGGTTCGGAAAAGAGGACTGCGTACGTTCGGCACTTTGCCAAGGTACGCAGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGCGCCCGGGCGAACAGGGCTTTGTTTTGGGTGGTTATGCCCGGACTAGCGCTTTTTCTGACACCACCATTTTTATTTTCCTCTGGCTGGTGAAgg                                                                                                                                                                                                                                         >  2:433781/1‑250 (MQ=255)
  ccTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGTAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTACGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAgagg                                                                                                                                                                                                                                       >  2:52992/1‑248 (MQ=255)
                          aGTGAAGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAAAAACCATTTTTATTTGCCTCTGGCTGGAGAAGTGTACGCTCATCCTGTGGCTTTTTTGt                                                                                                                                                                                                               >  2:454947/1‑251 (MQ=255)
                                            tttgaagaaAATGAAATGGTTGGGATCGGTCGGTTCAGTAAAGATGACTGCGTACGTTCGGCACTTTTCCAACGTATGCTGCATGCCTTCAGTTAAGTGTTTTATCGGTGCAGAGCCCGGGCGAACCGGGATTTGTTTTGGGTGTTTATGCCCGGGCTAGCGCTGTTTCTGAAACAACCATTTTTATTTGCCGCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCtc                                                                                                                                                                                              <  2:214699/247‑1 (MQ=255)
                                              ggaagaaGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCtcatc                                                                                                                                                                                           <  2:92489/250‑1 (MQ=255)
                                                           aTGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAg                                                                                                                                                                              <  2:361621/251‑1 (MQ=255)
                                                                  ggATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTg                                                                                                                                                                       <  1:149695/251‑1 (MQ=255)
                                                                      catCCGGTGCGGTAAAGAGGCCTGCGTACGTTCGGCCCTTTGCCCACGTCCGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCCGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGACACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACTCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCat                                                                                                                                                                    >  2:261271/3‑250 (MQ=255)
                                                                                       agGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATAtt                                                                                                                                                  <  2:113998/251‑1 (MQ=255)
                                                                                                                              aTGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGTCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCt                                                                                                           <  1:32947/251‑1 (MQ=255)
                                                                                                                                gCCTACAGTTAAGTGTGTTATCGGGGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCtt                                                                                                          <  2:224168/250‑1 (MQ=255)
                                                                                                                                     caggtAAGTGGGTTATCGGTGAAGAGACCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTGTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCCTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTa                                                                                                    <  2:426617/247‑1 (MQ=255)
                                                                                                                                       gTTAAGTGTGTAATCGGTGCAGAGCCCGGGCGAACCGGGCTATGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACa                                                                                                  <  1:79254/251‑1 (MQ=255)
                                                                                                                                                                           tccgatttttttGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAACTAACCATTGTTATTTGCCTCGGGCTGGTGAAGTGTGCGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATAAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGTTGTGTTTGAAAACGCAGCTAATTGTACAGCGCCCAGAAAATTATCACGACGGCATTCGCCACt                                                               <  2:428473/242‑1 (MQ=255)
                                                                                                                                                                                   ttttGGGAGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTATATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCAAGACAATTAACACGACGGCATTCGCCACt                                                               >  1:428473/1‑242 (MQ=255)
                                                                                                                                                                                       gggTGTTTATGCCCGGACTTGCGTTTGTTCAGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCaaaaaa                                                   <  2:266668/250‑1 (MQ=255)
                                                                                                                                                                                        ggTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAAt                                                                                     <  2:173761/215‑1 (MQ=255)
                                                                                                                                                                                        ggTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAAt                                                                                     >  1:173761/1‑215 (MQ=255)
                                                                                                                                                                                                      gACTAGCGCTTTATCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGATCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGATGCTGTTTTTGAAAACGCAGATTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAACAAATAGAACTGAAGTCGGc                                   >  1:117963/1‑251 (MQ=255)
                                                                                                                                                                                                         gtgcgcTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACTCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCAGCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGTATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCtt                                 <  2:450087/248‑1 (MQ=255)
                                                                                                                                                                                                                                       ttGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGCACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGTcc  >  1:476851/1‑250 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     
TGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGTCA  >  NC_000913/1085917‑1086398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: