Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,527	C→T	E405K (GAA→AAA)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,527	0	C	T	100.0%	82.3 / NA	27	E405K (GAA→AAA)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

GCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATC  >  NC_000913/822288‑822767                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 gCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCt                                                                                                                                                                                                                                       <  1:280697/251‑1 (MQ=255)       cATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGttt                                                                                                                                                                                                                                 >  1:270900/1‑251 (MQ=255)       cATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGttt                                                                                                                                                                                                                                 <  1:144418/251‑1 (MQ=255)                   aaaTCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCCTCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGtt                                                                                                                                                                                                                     >  2:57322/1‑251 (MQ=255)                                         caGCCCGACCGCACGAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTgcgc                                                                                                                                                                                               <  1:476427/251‑1 (MQ=255)                                           gCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGcc                                                                                                                                                                                              >  1:170665/1‑250 (MQ=255)                                                 ccGCACTAAGCAGCGCAACTCGGTTGGAGGCGCGGATGACCTTCCGGACCTCTACCCAGTCCACTTTTTT‑GGCTTATACCACCAGCAACACAATCACCGCGATAAAAGACAGCGAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGTGTGTGCTTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCGGAGTTTCCATTGTCGGTTGTGCGGGCGGTTCAACCTGCGCCAGAAGTg                                                                                                                                                                                       >  2:250155/1‑251 (MQ=255)                                                    cACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGt                                                                                                                                                                                                                                   <  1:272773/204‑1 (MQ=255)                                                    cACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGt                                                                                                                                                                                                                                   >  2:272773/1‑204 (MQ=255)                                                        aaGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCgtg                                                                                                                                                                                  <  2:233307/249‑1 (MQ=255)                                                            agcGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGc                                                                                                                                                                             >  1:78379/1‑251 (MQ=255)                                                                 aaCGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAACAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGACAGACGTGGCGTGGGGGCCGGa                                                                                                                                                                         >  2:472439/1‑249 (MQ=255)                                                                                      gACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATATTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGAGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGACGCCAGCCAACCAGCGCCgggg                                                                                                                                                   >  2:437156/1‑249 (MQ=255)                                                                                                         cAGTCCACTTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGaa                                                                                                                                <  2:78379/251‑1 (MQ=255)                                                                                                          aGTCCACTTTTTT‑GGCGTAGCACACCAGCAACACAATCACCGCGATAAAAACCAGCCAGGCGAGGATCTTCTTTGCTCAGCGCCAGCGCGGGCGTAATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTATCGGTTGTGCGGGCGGATCAACCAGCGCCCGCCGTGGCGTGTGTGCCGGCAGCGAGACAACCAGCGCCGGGacgaggggcaaacagcggaacg                                                                                                                              >  2:279273/1‑230 (MQ=255)                                                                                                               aCTTTTCT‑GGCGTAGACCACCAGCAACACAATCCCCGCGATAAAAAACAGCCAGGTGAGGATCTTCCTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTTCCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATTAACCCGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGTCGGGAAGTGGCGTAAAAAGTGGAACGCCAGc                                                                                                                         <  1:250155/251‑1 (MQ=255)                                                                                                                  ttttt‑GGCGTAAACCACCAGCAACACAAACACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCAc                                                                                                                       <  2:270900/250‑1 (MQ=255)                                                                                                                  ttttt‑GGCGGAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTGTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCAc                                                                                                                       <  2:46619/250‑1 (MQ=255)                                                                                                                              gACCACCAGCAACACAACCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAg                                                                                                           <  2:170665/251‑1 (MQ=255)                                                                                                                                                   cgcgATAAAAAACAGCCAGGCGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCACCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGAGGGGTAAAAAGTGGAAAGCCAGACCGCTTTAGGTAAGGTTCCACCAGGTGCGATTggg                                                                                      >  2:333522/1‑251 (MQ=255)                                                                                                                                                          aaaaaCAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGAGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGAGTGTGCCGGCAGCCGGCCAACCAGCGCCGGGAGGTGGCGTAAAAAGTGGAACGCCAGGCCGCTTTTGGTCAGGGTTCACCATGGGCGGTTGGGCAGCATg                                                                               >  2:377544/1‑251 (MQ=255)                                                                                                                                                                                      cTAAGCGCCAGCGCAGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCCGCCAACCAGCGCCGGGGAGTGGCGTAAAAAGTGGAACGCCCGCAAGCTTTTGGTCAGGGTCCCCCAGGGGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCgg                                                   >  2:181444/1‑251 (MQ=255)                                                                                                                                                                                                   cgGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGGACGCCCGCACGCTTTTGGTCAGGATCCACCCGGGGCGTTTGGGCAGCATGGGTTAATCCACCTGCTGCCAATCTGCGGCATTAATGCCGtt                                      >  2:98412/1‑251 (MQ=255)                                                                                                                                                                                                                  catcaGGGGTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAg                       <  2:148895/251‑1 (MQ=255)                                                                                                                                                                                                                                  ccGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTTCGAGGGCGGATCAACCTGCGCCAGACGTGGCGTGTATGCCCGAAGCCAAGCAACCAGCCCCCGGAAGTGGCGAAAAAAGTGGGACCCCCGCACGCTTTTTGTCAGGTTTCAACCGGAGAGTTTTGGCAGCCTGGCTTCATCCACCTGCTGGCAATCTTCGGCCATAATGCCGTTCAGATTATCGCGCCGCGTCTGGTTaaac          >  2:218675/1‑247 (MQ=255)                                                                                                                                                                                                                                   cGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGATCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGAc      <  1:98412/251‑1 (MQ=255)                                                                                                                                                                                                                                        gttCAGTGTTTACCCGATCCTGCGTTTCCATTGTCGGTTGGGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATc  <  2:84307/249‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 GCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTT‑GGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATC  >  NC_000913/822288‑822767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑