Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	780,624	C→T	noncoding (71/76 nt)	lysT →	tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	780,624	0	C	T	88.6%	106.8 / 2.5	44	noncoding (71/76 nt)	lysT	tRNA‑Lys
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base T (19/20);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTT  >  NC_000913/780385‑780850                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    tGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCt                                                                                                                                                                                                                          >  1:298487/1‑251 (MQ=255)     aTGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAACCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAgg                                                                                                                                                                                                                      >  1:229253/1‑251 (MQ=255)          tAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCCGTTGCCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTacatcgaa                                                                                                                                                                                                                  >  2:369043/1‑243 (MQ=255)            acacccGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCGACATCGAAATTTTTGTTGCGCTCAAGTCGGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGc                                                                                                                                                                                                              <  2:116230/251‑1 (MQ=255)            acacacGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGc                                                                                                                                                                                                              <  2:9663/251‑1 (MQ=255)                 cGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGgt                                                                                                                                                                                                           >  1:243245/1‑249 (MQ=255)                    ccAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAACCAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGCGGA‑GACGgtagta                                                                                                                                                                                                       >  2:195824/1‑250 (MQ=255)                    ccAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGACGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCAAAAGTAGA‑AGcagcagta                                                                                                                                                                                                       >  1:293621/1‑243 (MQ=38)                              cATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAgg                                                                                                                                                                                            >  1:469624/1‑251 (MQ=255)                                    ttCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGAGCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAAc                                                                                                                                                                                       <  2:298487/250‑1 (MQ=255)                                                      aaTCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGccc                                                                                                                                                                    >  1:129172/1‑251 (MQ=255)                                                               cAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCgg                                                                                                                                                           >  2:143915/1‑250 (MQ=255)                                                                aGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCgag                                                                                                                                                          <  1:243499/251‑1 (MQ=255)                                                                    gAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACg                                                                                                                                                       >  1:426870/1‑250 (MQ=255)                                                                       tGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGt                                                                                                                                                   >  1:166571/1‑251 (MQ=255)                                                                            tACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGCAAGAAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAGTCGCTAAGGCGGA‑AGCGGTGGTAAAACGAGACGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGCCGAGCTCGAGt                                                                                                                                              >  1:221783/1‑251 (MQ=37)                                                                             aCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTc                                                                                                                                                  <  2:307280/246‑1 (MQ=255)                                                                             aCATCGAAAATGTGGTTGCGCTCAAGTATGAAATCAGTAATATAGGCAGCCGTTGGAACGGGATATCAAAAAAACCGAAAGCAAAGAAAAAGGGGGTCTGTAGCTCAGTTGGTAGAGCAGTTGACGTTTAATCAATTGGGCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGTGACGAAGTCg                                                                                                                                                 <  2:467718/247‑1 (MQ=255)                                                                                 cGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATCTATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATcc                                                                                                                                         >  1:135072/1‑251 (MQ=255)                                                                                 cGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATcc                                                                                                                                         <  1:15720/251‑1 (MQ=255)                                                                                       ttttGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAc                                                                                                                                    <  1:233650/250‑1 (MQ=255)                                                                                       ttttGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACg                                                                                                                                   >  1:74008/1‑251 (MQ=255)                                                                                                            aaTCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACAtagttag                                                                                                              <  1:100612/251‑1 (MQ=255)                                                                                                                   aaTATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACgg                                                                                                                                                                       >  2:393028/1‑187 (MQ=255)                                                                                                                   aaTATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACgg                                                                                                                                                                       <  1:393028/187‑1 (MQ=255)                                                                                                                   aaTATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTgtag                                                                                                        <  2:166571/250‑1 (MQ=255)                                                                                                                    atatatGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTgtagt                                                                                                       >  1:299017/1‑250 (MQ=255)                                                                                                                      atatGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGCGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGGAGAGTTATTCTACACGACCCACCACTAAAATAGTGAGTTGTAGTATc                                                                                                    >  1:314233/1‑251 (MQ=37)                                                                                                                              ccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCgtag                                                                                            <  1:165799/251‑1 (MQ=255)                                                                                                                                  tGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTAt                                                                                         <  2:74008/250‑1 (MQ=255)                                                                                                                                    ccACGGGTTAACAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTGAGGTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCCCCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCgg                                                                                      <  2:392206/251‑1 (MQ=255)                                                                                                                                        gggATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGCAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAAAATAGTTtgttgtggtggacggagtttgaaggggcg                                                                                   >  1:200377/1‑227 (MQ=21)                                                                                                                                                         gAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGgaga                                                                 <  2:426870/251‑1 (MQ=255)                                                                                                                                                                    aaaaGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCtt                                                      <  1:143915/251‑1 (MQ=255)                                                                                                                                                                        gTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGAACGACTCACCAATCG‑TTAGGTGGAGAACGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAAAGGCCCGAAGGGCGAGAAGAAGTCGAGGACTCCTGCACGACACCCCACTAGCCTAGTTAGTTGTAGGATCCAGCGTCGTATCAGGAGATTAGCACAGCTGGGAGAGCTCCTCCTTTACa                                                   >  2:89088/1‑250 (MQ=18)                                                                                                                                                                              cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGCGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAggg                                            >  1:356080/1‑251 (MQ=255)                                                                                                                                                                                      aagtgggTAGTGCAGTTGACTTTTAATCAATGGGTCGCAGGTTGGAATCCGTCACGACTAACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGTTAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggcg                                    <  2:257401/246‑1 (MQ=38)                                                                                                                                                                                              agagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGgtagta                                                                                                                                                                                                       >  2:268281/1‑80 (MQ=255)                                                                                                                                                                                              agagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGgtagta                                                                                                                                                                                                       <  1:268281/80‑1 (MQ=255)                                                                                                                                                                                                      tGACTTTGAATCAATTGGCCGCAGGTGCGAATCCTGCTCTACTCACAAATCGCTAAGGGGGA‑AGCGGTAGTAAAACGTGAATGATAACGTGGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCCCCACTATCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCATCTGGGAGAGCACCTCCCTTACAAGTATGGGGTCGGCGGTTCTATCCCGtcat                     <  2:322483/250‑1 (MQ=255)                                                                                                                                                                                                           tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacc                 <  1:109141/249‑1 (MQ=255)                                                                                                                                                                                                             tAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacccac              <  1:151465/250‑1 (MQ=255)                                                                                                                                                                                                                     tGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCCGCCTCACCCACCACCGGGtg     >  2:354259/1‑250 (MQ=255)                                                                                                                                                                                                                          gCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGGTCGATCCCGTCATCACCCACCACCGGGGCGtt  >  2:199854/1‑249 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    TGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGA‑AGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTT  >  NC_000913/780385‑780850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑