Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,259	G→A	C175C (TGC→TGT)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,259	0	G	A	98.0%	150.1 / ‑6.4	50	C175C (TGC→TGT)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (26/22);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGTACAACCCATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCC  >  NC_000913/674013‑674493                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           ggtaCAACCCATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCagcaacagc                                                                                                                                                                                                                                        <  1:149334/251‑1 (MQ=255)  gtaCAACTCAAAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTAATAGTCGTTAACGTTGAAGGTGATATCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGGGATCCAGTTTAGCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGTCAGCAACAGcc                                                                                                                                                                                                                                       <  1:416157/251‑1 (MQ=255)       aCCCATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCg                                                                                                                                                                                                                                   >  1:412559/1‑250 (MQ=255)        cacagaaagggggccGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCACAGGCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACTCGGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATTGCAGCGCCAGCAACAGCCGTCGAt                                                                                                                                                                                                                                 <  2:17814/238‑1 (MQ=255)           aTAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGTTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGTGCAGCTCGTCAGCGTAAGCAGTGATTTTGAGAAACCACTGCGGGATCTCGTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCTATAAc                                                                                                                                                                                                                              <  2:444254/251‑1 (MQ=255)                 gtggccGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTgttc                                                                                                                                                                                                                        <  1:191690/247‑1 (MQ=255)                        ggcgggTAGTGTAAACGGTCCGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTgtttgttc                                                                                                                                                                                                                    >  2:152666/1‑248 (MQ=255)                        ggcgggTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTgttcgttc                                                                                                                                                                                                                    <  1:152666/248‑1 (MQ=255)                           gggTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGt                                                                                                                                                                                                              >  1:111633/1‑251 (MQ=255)                           gggTAGTGTAAACGGTCAGCGTGTTGTCATAGTAGTTAACGTTGAAGGTGATCTACACGACTTCGGAACGACCGATCCAGTTACGATGCATGGTTTTAACGGAGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGAGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGCTAAATTGTTCGTTCGCCAGt                                                                                                                                                                                                              >  1:16868/1‑251 (MQ=255)                              tAGTGTAAACGGTCAGCGTGTTGACATAGTCGTTAACGTTGAAGGTGCTCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGCTAAACCACTGCGGGTTCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCCACAGCCGTCGATAACTTGTTCGTTGGCCCGTACg                                                                                                                                                                                                           >  2:481392/1‑251 (MQ=255)                                         gTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGtt                                                                                                                                                                                                <  1:118658/251‑1 (MQ=255)                                               gtgtTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGCATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGc                                                                                                                                                                                           >  1:52867/1‑250 (MQ=255)                                                tgttgtCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCgg                                                                                                                                                                                             <  2:48194/247‑1 (MQ=255)                                                tgttgtCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCgg                                                                                                                                                                                             >  1:48194/1‑247 (MQ=255)                                                         aGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGAcc                                                                                                                                                                                >  2:97440/1‑251 (MQ=255)                                                         aGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGAcc                                                                                                                                                                                <  1:177179/251‑1 (MQ=255)                                                                aaCGTTGAAGGTGCTCTCCACTCCTTCGGCACGACCGCTCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACAACTGCGGGATCTCTTTAAGTTCAACTTTGGTATCGCAGCGCCAGCAACCGCCGTCGATAACTTGTTCATTCGCCAGGACGGTCTGGTCGTTCGGGCACCTGTTGCCCGc                                                                                                                                                                              >  2:204817/1‑246 (MQ=255)                                                                     tGAAGGAGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAAATCGTTGAGCAGCTCGACAGCGTAAGCAGTGATTTTGATAAAACACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAttcttc                                                                                                                                                                    >  2:298598/1‑251 (MQ=255)                                                                                    cGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCt                                                                                                                                                     >  1:284765/1‑251 (MQ=255)                                                                                    cGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGACTTCTTATATACCAGGCCt                                                                                                                                                     >  2:31978/1‑251 (MQ=255)                                                                                            gAACGACCGATCCAGTTACTCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTtata                                                                                                                                             <  1:453060/251‑1 (MQ=255)                                                                                              aCGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGTATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACa                                                                                                                                           <  1:384957/251‑1 (MQ=255)                                                                                              aCGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGACGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATAcc                                                                                                                                           >  1:433942/1‑250 (MQ=255)                                                                                                    gATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCgg                                                                                                                                     <  1:126441/251‑1 (MQ=255)                                                                                                                  gCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTg                                                                                                                        <  1:110948/250‑1 (MQ=255)                                                                                                                                 tgtCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTAt                                                                                                        >  1:191169/1‑251 (MQ=255)                                                                                                                                        ccAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCg                                                                                                 >  1:272130/1‑251 (MQ=255)                                                                                                                                        ccAGTGATCCAGTTTATCCAGAGTGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCg                                                                                                 <  1:298598/251‑1 (MQ=255)                                                                                                                                              aTCCATTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAgg                                                                                           <  2:131290/251‑1 (MQ=255)                                                                                                                                                               cGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTc                                                                          <  2:50383/251‑1 (MQ=255)                                                                                                                                                                gTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGCAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGCAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGcccc                                                                         >  2:167627/1‑248 (MQ=255)                                                                                                                                                                    agcagcTCGTCAGCGTAAGCAGTGATTTTGCTAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTc                                                                     >  2:74687/1‑251 (MQ=255)                                                                                                                                                                      cacctcgtcatcttaatctgTGATTTTGATAAACTCCTGCGGGATCTCTTTACTTTCAACTTTGGTAGCGCAGCGCCAGCAACAGCTGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTGCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTCATACAGCTCGGTGAAGAATTTCTTTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCATTCAt                                                                   <  1:268761/232‑1 (MQ=255)                                                                                                                                                                            gTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCa                                                              >  1:118146/1‑250 (MQ=255)                                                                                                                                                                            atCAGGGTAAGAAGTGATTTTGAAAAACCACTGCGGGATCTCTTAACGTTCAACTGGTGTACCGCAGCGACAGCAACAGCCGTCGATAACTTTTTCGTTCGCCAGTACGGTCTGGGCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTGCTGTTCCCAACGGTATTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCaa                                                             <  2:218766/250‑1 (MQ=255)                                                                                                                                                                                   tAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGccc                                                        >  1:53386/1‑249 (MQ=255)                                                                                                                                                                                   tAAGCAGGGTTTTTGATAAACCCCGGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCACCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCa                                                       <  2:272130/250‑1 (MQ=255)                                                                                                                                                                                       cAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCAtt                                                  >  2:80975/1‑251 (MQ=255)                                                                                                                                                                                                tcaTAACCCACTGCGGGATCTCTTTACGTTCACCGTTGGCATCGCAGCGCCAGCAGCAGCCGTCGTTAACTTGCTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTCTATACCAGGCCTTTTTTATACAGCTCGGTGAAGACTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATACCCAAAGCCAAGCATTTTGGGCTgg                                         >  2:55772/3‑251 (MQ=255)                                                                                                                                                                                                      gccACTGGGGGATCTCTTTACGTTCAACTTTGGTATGGCAGCGCCAGCAACAGCCTGCGATAGCTTGTTCGTTCGTCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATGCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCGAGCATTTTGAGCTGGttttac                                   <  2:162316/250‑3 (MQ=255)                                                                                                                                                                                                         ccTGCGGGATCGCTTTACGTTCAACTTGTGCATCGCAGCGCCAGCAACAGCCGTCGATAATTGGTTCGTTCGCCATTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCACGGTGAAGAATTTCTGTTCCCAACGGTAGTAGTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCata                                <  2:52867/250‑1 (MQ=255)                                                                                                                                                                                                                gATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGCGCTGGTTTTTCATATAcgcg                          >  2:141784/1‑250 (MQ=255)                                                                                                                                                                                                                  tctcTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGAtg                       >  1:78050/1‑251 (MQ=255)                                                                                                                                                                                                                    tctTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTACTATACGCGAtgtt                     >  2:173954/1‑251 (MQ=255)                                                                                                                                                                                                                         aCGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGAtgttttagt                >  2:251500/1‑246 (MQ=255)                                                                                                                                                                                                                                   ttGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCAc        <  1:31978/249‑1 (MQ=255)                                                                                                                                                                                                                                     ggTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGt     <  1:363349/250‑1 (MQ=255)                                                                                                                                                                                                                                     ggTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGCACGGTATGGTCGTTCGGGCACCAGTTGACAGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGAGATGTTGTCGTACGTCCACGGTg    >  1:298474/1‑251 (MQ=255)                                                                                                                                                                                                                                       tATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGcc  >  2:106397/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGcc  <  1:141784/250‑1 (MQ=255)                                                                                                                                                                                                                                                  cagcaacagCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGtt                                                                                         >  1:469561/1‑153 (MQ=255)                                                                                                                                                                                                                                                  cagcaacagCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGtt                                                                                         <  2:469561/153‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           GGTACAACCCATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCC  >  NC_000913/674013‑674493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑