Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 602,835 G→A A293T (GCT→ACT)  pheP → phenylalanine:H(+) symporter PheP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913602,8350GA98.4% 190.0 / NA 62A293T (GCT→ACT) phePphenylalanine:H(+) symporter PheP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (33/28);  minor base C (1/0);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CGCTGGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAGCTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGG  >  NC_000913/602599‑603081
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      
cGCTGGCGGTAATTATGTTCTCCTTCGGCGGGCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAACGGTAAATCAGGTGGGGTATCGCATCCTTCTGTTTTTCATCGGTTCGCTGGTGGGTTTTCTTGCGCTCTATCCGTGGGTGGAAGGGAAATCCAACAGTAGCCCGTTTGTGGTGATTTTCCCTAATCTCGACAGCCACGTGGTAACCTCTGCGCCGAAc                                                                                                                                                                                                                                          >  2:119879/1‑251 (MQ=255)
 gCTGGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACt                                                                                                                                                                                                                                         <  2:248308/251‑1 (MQ=255)
       ggTAATTATGGTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGTCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCa                                                                                                                                                                                                                                   <  1:115436/251‑1 (MQ=255)
          aaTTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCAtt                                                                                                                                                                                                                                 <  1:392383/250‑1 (MQ=255)
                     ccgtCGGCGGTCTGGAGCTGATTTGGATTTCTGCCGCGGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACATCAACGTGGTAACCTCTGCGCTGAACTTCGTCATGCTGGTAGCAt                                                                                                                                                                                                                       <  2:483273/246‑1 (MQ=255)
                     ccgtCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGGTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCa                                                                                                                                                                                                                        <  1:223350/245‑1 (MQ=255)
                     ccTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCa                                                                                                                                                                                                                        <  1:238934/248‑1 (MQ=255)
                           gcggTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTc                                                                                                                                                                                                                >  2:377699/1‑250 (MQ=255)
                           gcggTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCa                                                                                                                                                                                                               <  1:424776/251‑1 (MQ=255)
                             ggTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAgt                                                                                                                                                                                                             >  1:369198/1‑251 (MQ=255)
                                  ggAGCTGATTGGGATTACTGCCGCTGAATCGCGCGAGCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGTTGGTTTTACTGGCGCTCTTTCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATTATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCGGTCAGTGta                                                                                                                                                                                                          <  1:404886/249‑1 (MQ=255)
                                  ggAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAgtgt                                                                                                                                                                                                           <  1:62991/248‑1 (MQ=255)
                                  ggAGCGGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGta                                                                                                                                                                                                          <  2:407787/249‑1 (MQ=255)
                                       ggATTGGGATTGCTGCCGCTTAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATGCTGGTAGCATCGCTGTCAGTGTATAACAGc                                                                                                                                                                                                   <  2:224191/250‑1 (MQ=255)
                                         aTTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCgg                                                                                                                                                                                                 >  2:128181/1‑251 (MQ=255)
                                             ggATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGtt                                                                                                                                                                                             >  1:339970/1‑251 (MQ=255)
                                                         cTGAAGCGCGCGATCCGGCAAAAAGCATTCCCAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACCTCGGTTCCCTGGTGGTTTTAATGGCGCTCTATACGGGGGGGGAAGTGCGATCAAACAGTAGCCCGTTTGTGCTGCTTTTCCCTAATCTCGACAGCAACGTGGTAACTTCCGCGCTGAATTTCGTCATTCTGGTAGCATCACTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGc                                                                                                                                                                                 >  2:291324/1‑251 (MQ=255)
                                                                     aTCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTTGTGTATCGCTTCCTGCTGTGTTACATCGGTTCACTGGTGTTTTTACTGGCGCTCTATCCGTGTGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGTTAACTTCTGCGCTGAACTTCGTCATTCTGGTATCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGttt                                                                                                                                                                     <  1:305441/251‑1 (MQ=255)
                                                                            aaaaaGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCCTCGCTGTCAGTGTATAAAAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCttt                                                                                                                                                              >  2:413607/1‑251 (MQ=255)
                                                                               aaGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCgggg                                                                                                                                                                                               >  2:232750/1‑215 (MQ=255)
                                                                               aaGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCgggg                                                                                                                                                                                               <  1:232750/215‑1 (MQ=255)
                                                                                           aGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATg                                                                                                                                               <  1:39515/251‑1 (MQ=255)
                                                                                                 aaaTCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCg                                                                                                                                          >  1:62038/1‑250 (MQ=255)
                                                                                                     cAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTTCTGGCGCTCTATCCGTTGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGtt                                                                                                                                     <  1:477679/251‑1 (MQ=255)
                                                                                                       ggtggaGTATAGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATACAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGGACCTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCCGGGTATAACAGCGGGGTTTACTCTAACAGCCGCATACTGTTTGGCCTTTTTGTGAAGGGTAATGGGCCGAAGtttt                                                                                                                                   >  2:301647/1‑251 (MQ=255)
                                                                                                                   cATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGGTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTc                                                                                                                        >  2:415295/1‑250 (MQ=255)
                                                                                                                       ctgctgTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCg                                                                                                                                                                                                                     <  1:320736/153‑1 (MQ=255)
                                                                                                                       ctgctgTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCg                                                                                                                                                                                                                     >  2:320736/1‑153 (MQ=255)
                                                                                                                                   aTCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGcc                                                                                                       >  1:434792/1‑251 (MQ=255)
                                                                                                                                   aTCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGcc                                                                                                       >  1:73847/1‑251 (MQ=255)
                                                                                                                                     cGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATATTCTCGACAGCAACGTAGTAACTTCTGCGCTGAACTTCGTAATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGAATGCTGTTTGGCCTTTCTGTGCAGGTTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCg                                                                                                      >  2:409082/1‑250 (MQ=255)
                                                                                                                                               gtggtTTTTCTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCGCGTTTGTGATGGTTTTCCGTGATCTCGAAAGCAACGTGGTAACTTCTGCGCTGCACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAAACGCGGGGTTTAATATATAAGCAGCATGCTGTTGGGCATTTCTGTGCAGGGTTATGCGCCGAAGTTTTTGACACGCGTCAGCAGTCGCGGTGTGCGGCTTAACTCgtt                                                                                           >  2:282144/1‑249 (MQ=255)
                                                                                                                                                 ggtTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTg                                                                                          >  1:24599/1‑250 (MQ=255)
                                                                                                                                                  gtTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGAt                                                                                        <  1:324776/251‑1 (MQ=255)
                                                                                                                                                      tACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCt                                                                                     <  1:117573/250‑1 (MQ=255)
                                                                                                                                                       aCTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCttt                                                                                   <  1:119879/251‑1 (MQ=255)
                                                                                                                                                        cTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTc                                                                                  >  1:380133/1‑251 (MQ=255)
                                                                                                                                                              ctctATCAGTGGGTGGAAGTGAAATCCAACAGTAGCCCGATTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGCACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTACCAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAGTGCGCCGCAGTTTTTGACTCGCGTCATCCGTCGCGGTGTGCCGATTAACTCGCTGCTGCTTTCCggtgc                                                                            >  1:399701/1‑248 (MQ=255)
                                                                                                                                                                     cGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACt                                                                     <  1:66818/251‑1 (MQ=255)
                                                                                                                                                                       gtggtggAAGTGAAATCCAACAGTTGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCACCGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCCTCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTGTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACtgc                                                                   <  2:361788/249‑3 (MQ=255)
                                                                                                                                                                       ggggtggAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTc                                                                   <  2:62038/250‑1 (MQ=255)
                                                                                                                                                                        gggtggAAGTAAATTCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCACCGTGGTCACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTCCTCTAACAGCCGCATACTGTTTGGACTTTGTGTGCAGGGTACTTCTCCGGAGTTTTTGACTCGCGTCATCCGTCGCAGTGTGCCGATTACCTCGCTTATCCTTTCCTGAGCGCTCACTTcc                                                                  >  2:229945/1‑250 (MQ=255)
                                                                                                                                                                               aGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCtggtgg                                                           >  1:294473/1‑251 (MQ=255)
                                                                                                                                                                                 tGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCACTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTTTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGCTTCACTCGCTGATGCTTTCCTGAGCGATCACTTCGCTGgtggtg                                                         >  2:104288/1‑251 (MQ=255)
                                                                                                                                                                                   aaaTCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAGCGTGTTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCTGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGtt                                                       <  1:34520/251‑1 (MQ=255)
                                                                                                                                                                                     aTCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTa                                                      >  1:365101/1‑250 (MQ=255)
                                                                                                                                                                                       ccAACAGTAGCCCGTTTGTGCTGATTTTCCATAATCTCGACAGCAACGTGTTAACTTCTGAGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATTCGCCGACGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAAt                                                    >  2:156080/1‑250 (MQ=255)
                                                                                                                                                                                                  ccGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGACGTCGCGGTGTGACGCTTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGATGGTGGTGTTAATCAACTATctgct                                        >  1:284861/1‑251 (MQ=255)
                                                                                                                                                                                                    gTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCtgctgc                                      >  2:197558/1‑251 (MQ=255)
                                                                                                                                                                                                      ttGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGcc                                     <  1:197558/250‑1 (MQ=255)
                                                                                                                                                                                                          gatgatTTTCCATAATCTCGCCAGCAAAGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGACGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCCCTTCGATGGTGGTGTTAATAAACTATCTGCTGCCgaca                                 >  2:463857/1‑247 (MQ=255)
                                                                                                                                                                                                             gatTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAg                             <  1:75346/251‑1 (MQ=255)
                                                                                                                                                                                                              atTTTCCATACTATCGACAGCACCGTGGAAACTTCTGCGCTGAACTTCGTAATTCTGGTAGCATCGCTATAAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATACTGTTTGGCCTTTCTGTGCCGGGTAATGCGCCGAAGTTTTTGACTCGCGTCCGCCGTCGCGGAGAGCCGATTAACTCGCTGATGGTTTCCGGACGGATCCCTTCGCTGGTGGTGTGAATCAACTATCTGCTGCAGCaaaa                               >  2:332438/1‑248 (MQ=255)
                                                                                                                                                                                                                tttCCATAATATCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGt                          >  1:274827/1‑251 (MQ=255)
                                                                                                                                                                                                                    cATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTgg                      >  1:466066/1‑251 (MQ=255)
                                                                                                                                                                                                                       aaTCTCGACATCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGTTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTct                   <  2:73847/251‑1 (MQ=255)
                                                                                                                                                                                                                             gACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGAt             >  1:419727/1‑251 (MQ=255)
                                                                                                                                                                                                                               cAGCAACGTGGTAACTTCTGCGCTGAACTTCGTAATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATgg           >  1:319805/1‑251 (MQ=255)
                                                                                                                                                                                                                                aGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGCCTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGgc          >  2:37248/1‑251 (MQ=255)
                                                                                                                                                                                                                                       tGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGcc                                                                                                       >  1:232228/1‑151 (MQ=255)
                                                                                                                                                                                                                                       tGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGcc                                                                                                       <  2:232228/151‑1 (MQ=255)
                                                                                                                                                                                                                                        ggTAACTTCTGCGCTTAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCTCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCGGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCtggtgg  <  1:409082/251‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      
CGCTGGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAGCTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGG  >  NC_000913/602599‑603081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑