Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	534,742	G→A	G276D (GGT→GAT)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	534,742	0	G	A	98.2%	161.9 / ‑4.5	56	G276D (GGT→GAT)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (31/24);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

GAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGC  >  NC_000913/534495‑534978                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             gAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGatat                                                                                                                                                                                                                                           <  1:249086/251‑1 (MQ=255)                  gTTAATCCAGGCCGAACGTCCGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTAAGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCt                                                                                                                                                                                                                           >  1:258952/1‑250 (MQ=255)                  gTTAATCAAGGCCGAACGTCCGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGCCCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCCGCTGACATGGTGTTTGATATCGGTAACCGTTTTGCt                                                                                                                                                                                                                           >  2:70292/1‑250 (MQ=255)                     aaTCCAGGCCGAACGTCCGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGGGTTTGATATCGGTAACCGTTTTGCTAAcc                                                                                                                                                                                                                       >  2:225398/1‑251 (MQ=255)                      agccAGGCCGAACGGCCGGTGAG‑GTTGGCCGGGGGCGGGGTAATTAATGCTGTCGCAGCCGCACTGTTACATCAGTTTGCTGAACTGTCCAGCGTT‑CCGGTGATCCCAACGCTAATGGGGTGGGGCTGTACCTCGGACGATCATGATCTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCg                                                                                                                                                                                                                      <  2:285402/249‑1 (MQ=255)                        ccAGGCCGAACGTCCGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGAGTTTGATATCGGTAACCGTTTTGCTAACCGTc                                                                                                                                                                                                                    >  1:8274/1‑251 (MQ=255)                          aGGCCGAACGTCCGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCCTGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCCTCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGGCAt                                                                                                                                                                                                                  >  2:14115/1‑251 (MQ=255)                               gAACGTCCGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGAAATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCgg                                                                                                                                                                                                             >  1:11920/1‑251 (MQ=255)                               gAACGTCCGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGGGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAAACGTCATACCgg                                                                                                                                                                                                             >  1:155735/1‑251 (MQ=255)                                      cGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGCTGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGatat                                                                                                                                                                                                                                           >  2:442563/1‑214 (MQ=255)                                      cGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGatat                                                                                                                                                                                                                                           <  1:442563/214‑1 (MQ=255)                                                   ccGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACAcc                                                                                                                                                                                          <  2:439478/251‑1 (MQ=255)                                                           ggggTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGgcg                                                                                                                                                                                  >  1:346830/1‑251 (MQ=255)                                                           ggggTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGGAGAGAAATACCCCGACGGgcg                                                                                                                                                                                  >  2:212239/1‑251 (MQ=255)                                                                     atGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGtt                                                                                                                                                                        <  1:472423/251‑1 (MQ=255)                                                                     atGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGtt                                                                                                                                                                        >  1:15681/1‑251 (MQ=255)                                                                                   gCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATAAAACAAAGGGCGCCAAATAGTTCatattgatattgg                                                                                                                                                          >  2:10647/1‑250 (MQ=255)                                                                                         ttACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGAc                                                                                                                                                    >  1:221339/1‑251 (MQ=255)                                                                                           tcaacaGTTTGCTGAACTGACCAG‑GTTGCAGGTGATCCCAACGCGAAGGGGGTGGGGCTGTATCCCGGAAGATCATGAACTGATGGCCGGGATGGTTGGTCTGCAAACCGCGCAGCGTTACGGTAACGCATCGCAGCTGTCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCGCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACg                                                                                                                                                   <  2:233776/249‑1 (MQ=255)                                                                                                    tGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGAGCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGt                                                                                                                                          <  2:375732/250‑1 (MQ=255)                                                                                                     gCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTcg                                                                                                                                        >  1:69675/1‑251 (MQ=255)                                                                                                      cTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGACCTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGGAGAGAAGTACACCGAAGGGCGCAAAATCGTTCATATTGATATTTAGCCGACGCAAATTGGgcgc                                                                                                                                       >  2:229475/1‑251 (MQ=255)                                                                                                               ccAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGAGTGCTgtgt                                                                                                                              <  2:307229/251‑1 (MQ=255)                                                                                                                cAGCGTT‑CCGGTGATCCCAAAGCTAATGGGCTGGGGCTGTATCCCGGACGATCCTGAACTGAAGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGAGTTTGATATCGGTAACCGTTTTCCTAACCGTCGTACCGGCTCGGGAGAGAAATACACCGCAGGGCGCAAAATCGTTCATATTGCTATTGAGCCGCCGCACACTGGTcgcggtctgggtc                                                                                                                             >  2:133919/1‑242 (MQ=255)                                                                                                                 aGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTcc                                                                                                                            <  1:14115/251‑1 (MQ=255)                                                                                                                        ccGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGGGAAATACACCGAAGGGCGCAAAATAGTTCATATTTATATTGAGCCGACGCAAATTGGTCTGGTGCTGTGTCCGGAtct                                                                                                                      >  1:373574/1‑251 (MQ=255)                                                                                                                                  cAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGACGGGCGCAAAATCGTTc                                                                                                                                                                       >  1:390101/1‑192 (MQ=255)                                                                                                                                  cAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGGTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTc                                                                                                                                                                       <  2:390101/192‑1 (MQ=255)                                                                                                                                         aaTGGGCCGGGGCTGTATCCCGGACGATCAGGAACTGATGGCCGGGATGGGTGGTTTGCAAACCGCGCATCGTTTCGGTAACGCAACGCTGCTGGCGCCTGACATGGGGTTTGATATCGGTAACCGTGTTGCTAACCGTCATACCGGCTCGTTAGAGAAATACCCCGAAGGGCGCAAAATCGTGCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGGGTCCGGATCTCGGTTTTGTCTCTGATg                                                                                                     <  2:468775/251‑1 (MQ=255)                                                                                                                                             ggctggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTa                                                                                                  >  1:144442/1‑250 (MQ=255)                                                                                                                                              gtgtgggctgTATCCCGGACGTTCATGAACTGATGGCCGGTATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGATCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTaaa                                                                                                <  2:25321/247‑1 (MQ=255)                                                                                                                                                  gggcttTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAgcgg                                                                                            <  2:24130/251‑1 (MQ=255)                                                                                                                                                     ctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGgcgc                                                                                         >  2:126497/1‑251 (MQ=255)                                                                                                                                                               gACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGAtctc                                                                                                                     >  1:219257/1‑213 (MQ=255)                                                                                                                                                               gACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGAtctc                                                                                                                     <  2:219257/213‑1 (MQ=255)                                                                                                                                                                    tCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCCAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCCAAAGCGGCGCTGACACTGCTGGTTg                                                                          >  2:123349/1‑251 (MQ=255)                                                                                                                                                                        gAACTGATGGCCGGGATGGTGGGTCGGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGGTTTGCTAACCGTCATACCGGCTAGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGGCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGt                                                                      <  2:329428/251‑1 (MQ=255)                                                                                                                                                                            ggctGGCCGGTTTGGTGGGTCTGCAAACCGCCCATCGTTACGGTAACGCAACGCTGCTGGCGTTTGACATGGTGTTTGATATCGGTAACCGTTTTTCTATCCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGCTATTGAGCCGGCGCAAATTGGTCGCGTGCTGTTTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTTCTGGTTGAAGTGgcg                                                                  <  2:381978/248‑1 (MQ=255)                                                                                                                                                                              aTGGCAGGGATAGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTGTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCACAATCGTTCATATTGATGTTGAGCCGACGCAAATTGGACGAGTGCTGTGTCAGGATCACGGTATTGGCTCTGTTGCTCAAGCTGCGCTGACCATGCCGGGTGCAGTGgcgcg                                                                >  2:231996/1‑250 (MQ=255)                                                                                                                                                                                ggCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCTTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAg                                                               <  2:258952/250‑1 (MQ=255)                                                                                                                                                                                  ccGGGATGGTGGGTCTGCAACCCGCGCATCGTTACGGTTACGCAACGCTGCTGGTGTCTGACATGGTGTTTGACATCGGTAACCGGTTTGCTAACCGTCATCACGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATACTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGgag                                                            <  2:147511/251‑1 (MQ=255)                                                                                                                                                                                   cGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAgg                                                              >  1:156567/1‑248 (MQ=255)                                                                                                                                                                                           tggGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGTCACTGCTGGTTGAAGTGGCGCAGGAGATGCaaaa                                                    >  2:85693/1‑250 (MQ=255)                                                                                                                                                                                           ggggtcgttcaaCCGTGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCaaaaa                                                   <  2:377130/241‑1 (MQ=255)                                                                                                                                                                                                 tGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatattgata                                                                                                                                                              <  1:246467/138‑1 (MQ=255)                                                                                                                                                                                                          cgcATCGTTACGGTAACGCCACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGACGGGCGCAAAAACGTTCATATTGGTATTTAGCCGACGCAAATTGGTCCCGTGCTGTGTCCGGCTCTCGGTATTGTCTCTGTTGCTAAAGCGGCACCGACACTCCTGTTTTACGTGGCGCATGCGATGCAAAAAGAGGGTCGACTGCCg                                    >  2:191897/1‑251 (MQ=255)                                                                                                                                                                                                            cATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTAATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGACCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTAGTCTGCCgtg                                  >  1:230002/1‑251 (MQ=255)                                                                                                                                                                                                              tCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCCAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCGCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTccgccggtgt                                >  2:134017/1‑247 (MQ=255)                                                                                                                                                                                                                    cGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTaa                            >  1:203110/1‑249 (MQ=255)                                                                                                                                                                                                                       aaacgcaacgcTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCTCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAAt                       <  2:156567/250‑1 (MQ=255)                                                                                                                                                                                                                         acgcaacgcTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATgg                     <  1:70292/251‑1 (MQ=255)                                                                                                                                                                                                                             aacgcTGCTGGCGTCTGACATGGTGTTTGATACCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGAGATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGGCTGCCGTGTCGTAAAGAATGGGTCg                 <  1:342794/251‑1 (MQ=255)                                                                                                                                                                                                                                   gctgGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACt           >  1:290396/1‑251 (MQ=255)                                                                                                                                                                                                                                   gctgGCGTCTGACATGGGTTTTGATATCGGTAACCGTGTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATGGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACt           <  2:203110/251‑1 (MQ=255)                                                                                                                                                                                                                                    ctgGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTg          >  1:329225/1‑251 (MQ=255)                                                                                                                                                                                                                                     tgGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGc         >  1:260894/1‑251 (MQ=255)                                                                                                                                                                                                                                       gcggcTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCca       <  2:15681/247‑1 (MQ=255)                                                                                                                                                                                                                                        cGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGcc        >  1:347383/1‑249 (MQ=255)                                                                                                                                                                                                                                             gACAGGGTGTTTGATATCGGTAACCGTTTTGCTAACCTACATACCGGTGCGGTAGAGAAATACACCGATGGGCGCAAAATGGTTCAGATTGATATGTAGCCGACGGAAATGGGTCGCGGGTTTTGTCCTTATCGCGGTATTGTCTCTGATGCTAAAGTGGCTCTGTCACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGTTCGTCTGCCGTGTCGTAATGAATGGGTCGCCGTCTGCCagcagc  <  2:485156/250‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             GAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTG‑CCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTT‑CCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGC  >  NC_000913/534495‑534978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑