Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	495,622	C→T	T168I (ACC→ATC)	htpG →	chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	495,622	0	C	T	95.9%	141.2 / ‑6.0	51	T168I (ACC→ATC)	htpG	chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (24/23);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAC‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAA  >  NC_000913/495378‑495859                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               cgcgACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑Caa                                                                                                                                                                                                                                            >  1:412266/1‑248 (MQ=255)        aaGTGATTGACCAACTGGGGACTATCGCTAAATCCGGTACCAAATCATGCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGt                                                                                                                                                                                                                                  <  2:316186/251‑1 (MQ=255)          gtgagtgaCCTTCTGGGGACACTCGTTAAATCCGGGACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTGGGTGTTGGTTTCTACGCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGTCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTgg                                                                                                                                                                                                                                <  2:237665/251‑1 (MQ=255)                 cccATCTGGTGACTAACGCTAAATCCGTTTCCAAATCATGCCTCTAATCCCTGTGTTCTGACCAGGCGAAATACAGCCAGCTGATCGGTCAGTTTGGTGTTCGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCTCGCTGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACTCCGTTGCCGACATCAT‑CAAAGAAGATCTTGGTTCTGaa                                                                                                                                                                                                                         <  2:451344/250‑1 (MQ=255)                 aCCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGaa                                                                                                                                                                                                                         <  1:308422/251‑1 (MQ=255)                   cATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAAt                                                                                                                                                                                                                       >  1:113222/1‑251 (MQ=255)                    aTCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGACTACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATc                                                                                                                                                                                                                      >  1:302813/1‑251 (MQ=255)                          ggACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATAAACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTAATGAAATCACCCTg                                                                                                                                                                                                                >  1:80774/1‑251 (MQ=255)                           gACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGc                                                                                                                                                                                                               <  1:86045/251‑1 (MQ=255)                               aTCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCt                                                                                                                                                                                                           >  1:159232/1‑251 (MQ=255)                                          cGGTACCAAATCATTCCGCGAAGCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGGGTTGGTTTCTACGCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCg                                                                                                                                                                                                <  2:302813/251‑1 (MQ=255)                                              gccAAAGCATGCCGCGAATCCCTGGGTTCGGACCAGGCTAAAGACATCCAGCTGAGCGGACAGTTTGGTGGTGGGTTCGACTCTGCGTGTAACGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATTGCTTCTCCTGGGAAGCGGCGGGCGAAGGTGAATACACCGTTGCCGACAGCAC‑CAAAGAAGATCGTGGTACTGAAAACACCCTGTATCTGCGTGAAGGCTAAg                                                                                                                                                                                             <  2:64104/249‑1 (MQ=255)                                                cAAATCATTCCTCGAATACCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAACACCAGAAAATGGCGTCTTCTGGGAGTCGGCTGGCGAAAGTGAATACACCGTTGCCGACATCAT‑AAAAGAAGATCGGGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGacgacg                                                                                                                                                                                          >  1:485418/1‑251 (MQ=255)                                                      aTTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTcc                                                                                                                                                                                    >  1:238249/1‑251 (MQ=255)                                                          cTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCgc                                                                                                                                                                                >  1:9914/1‑250 (MQ=255)                                                          cTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGCTGGTTTCTACTCTGCGTTTATCGCGGTCGCCACACTGACCGTGTGTACTCGCGCGGCAGGCGAACAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGACGGTGCATACACCGATGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCgc                                                                                                                                                                                >  1:435223/1‑250 (MQ=255)                                                               aTCCCTGGGATCTGACCAGGCGCAAGACAGCCAGCTGATCGGTCCGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTTCTGCCAT‑ACTCAAAACAGATCGTGGGACTGACATAACCCTGCATATTCGTGAAGGAGAACACGGGTTCCTCGATGAcc                                                                                                                                                                           >  2:337271/1‑250 (MQ=255)                                                                      ggTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGcgcgg                                                                                                                                                                    >  1:108728/1‑250 (MQ=255)                                                                        ttCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGAAAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGGGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGGTAGAGGGACTGAAATCACCCGGCATCTGCGCGAAGGCGAAGACGAGTTCCTCGCTGAcacgcgcgcgg                                                                                                                                                                  >  2:390543/1‑241 (MQ=255)                                                                            gACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTCGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCATGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCGGGCGAAGGTGAATACACCGTTGCCGACAGCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCGGCAGCTGCGTGAAGGCGAAGACGAGTTCCTCGATTACTGGCGCGTGCGTTc                                                                                                                                                              <  2:247179/251‑1 (MQ=255)                                                                              ccAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGCCTGGCGCGTGCGTTcc                                                                                                                                                             >  1:443486/1‑250 (MQ=255)                                                                               cAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGAACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTccct                                                                                                                                                           >  1:310400/1‑249 (MQ=255)                                                                                                  tGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGAc                                                                                                                                        >  1:138307/1‑251 (MQ=255)                                                                                                        ggcAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCat                                                                                                                                     <  2:467309/246‑1 (MQ=255)                                                                                                           gttgatgtgtgtgTTTCTGCTCGGCGTTTATCGTGGCCAACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAGGGCGTCTTCTGGGAATCGGCTGGCGAAGGGTAATACGCCGTTGCCGACATCAA‑CAAAGAAGATCGTGGTACTGACATCACCCTGCATCTTCGTGAAGTCGAAGACGAGTTCCTCGATGACGGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATcgcg                                                                                                                               <  2:245126/239‑1 (MQ=255)                                                                                                                   gtTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGt                                                                                                                       <  1:88226/251‑1 (MQ=255)                                                                                                                     tGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGt                                                                                                                       >  1:420338/1‑249 (MQ=255)                                                                                                                         ttCTACTCGGCGTTTAGCGTGGCCGACAAAGTGATAGGTCGTACTCGCGCGGCAGGCTAAAAACCAGAAAATGGCGTCGTCGGGGAAGCGGCGGGCGAAGTTGAAGACACCGTGGCCGACATCAT‑CAAAGAAGATCGTGGGTCGGAAACCGCCCTGCGTCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGGGCGTTCCATCATCAGCAAATACTCCGTCCATATCGCGTTGCCGGTAgaga                                                                                                                  <  2:485418/250‑1 (MQ=255)                                                                                                                                     tttATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACt                                                                                                                                             <  2:154617/211‑1 (MQ=255)                                                                                                                                     tttATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACt                                                                                                                                             >  1:154617/1‑211 (MQ=255)                                                                                                                                        aTCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGa                                                                                                     >  1:35536/1‑248 (MQ=255)                                                                                                                                           gTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGGAAGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGGACTGAAATCACCCTGCCTCTGCGGGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGAAAATACTCCGACCATAACGCGCCGCGGGTGGGGATCGAAAAACGCGAagagc                                                                                                >  2:329146/1‑249 (MQ=255)                                                                                                                                            tGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGGGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGGAGAGATCGAAAAACACGAAGGGaa                                                                                               >  2:117564/1‑250 (MQ=255)                                                                                                                                             ggCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGaaa                                                                                              <  1:478348/250‑1 (MQ=255)                                                                                                                                             ggCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGaa                                                                                               >  1:143469/1‑249 (MQ=255)                                                                                                                                                         gACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGACGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCg                                                                                                               <  1:269238/221‑1 (MQ=255)                                                                                                                                                               gCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTAtc                                                                            <  2:412266/250‑1 (MQ=255)                                                                                                                                                                         gcgGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGaa                                                                 <  1:135446/251‑1 (MQ=255)                                                                                                                                                                                 cGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATcaaca                                                         <  2:159232/251‑1 (MQ=255)                                                                                                                                                                                  gAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGGAGACTGGCGCGTGCGTTCCATCCTCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACACGAAGAGAAAGCAGGCGAAACCGTTATCTCCTAGGAGaaacacaaaa                                                         >  2:15738/1‑243 (MQ=255)                                                                                                                                                                                                tGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CCAAGCAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGCAGACGCGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCt                                                                                                                             >  1:242365/1‑168 (MQ=255)                                                                                                                                                                                                tGGCGTCTTCGGGGAAGCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTTGCGCGTGCTGTCCATCATCAGCAAATACTCCGACCTTATCGCGCt                                                                                                                             <  2:242365/168‑1 (MQ=255)                                                                                                                                                                                                           gggAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTa                                 <  1:117564/249‑1 (MQ=255)                                                                                                                                                                                                                 cGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCGCCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCg                         <  2:36835/251‑1 (MQ=255)                                                                                                                                                                                                                            ggTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTTCTGAAATCACCCGGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCAACAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGa              <  2:323261/251‑1 (MQ=255)                                                                                                                                                                                                                             gTGAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGAt             >  1:200634/1‑251 (MQ=255)                                                                                                                                                                                                                               gAATACACCGTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATg            <  1:8745/250‑1 (MQ=255)                                                                                                                                                                                                                                 aTACCCCGTTGCCGACAGCAT‑CAAAGATGATCGTGGTACTGAAATCTCCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATAGCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAag         <  2:323125/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGTTGCCGACATCAA‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACa   <  2:35536/251‑1 (MQ=255)                                                                                                                                                                                                                                        gTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACa   >  1:84977/1‑250 (MQ=255)                                                                                                                                                                                                                                        gTTGCCGACATCAT‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAGCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGATTACaa  <  1:337271/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               CGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAC‑CAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAA  >  NC_000913/495378‑495859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑