Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	472,849	A→G	G84G (GGA→GGG)	glnK →	nitrogen regulatory protein PII‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	472,849	0	A	G	97.4%	105.0 / NA	38	G84G (GGA→GGG)	glnK	nitrogen regulatory protein PII‑2
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (17/20);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGC  >  NC_000913/472604‑473086                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              cTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAAt                                                                                                                                                                                                                                          >  1:173871/1‑251 (MQ=255)    gTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTCTCTTCAATTGGAATTCAGGTCCTGACCGTCACCGAAGTAAAAGGTTTCGGGCGTCAGAAAGGGCATGCAGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAACGTAAACATTGATGTGGCGATTGATGATGAACAACTCGCTGAAGTGATCGATATCGTAAGTAAGGCGGCTTACACCGGGAAAATTgg                                                                                                                                                                                                                                       >  2:400255/1‑251 (MQ=255)       aCCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCg                                                                                                                                                                                                                                     >  2:202007/1‑250 (MQ=255)         cGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGTTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACg                                                                                                                                                                                                                                  <  2:62705/251‑1 (MQ=255)               aaTCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCaaaa                                                                                                                                                                                                                            <  1:86447/251‑1 (MQ=255)                        aTTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTtcgtcg                                                                                                                                                                                                                   <  1:436434/251‑1 (MQ=255)                            aaGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTg                                                                                                                                                                                                                >  1:77013/1‑250 (MQ=255)                                tGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCACGTCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTCCCGGGGGGCGGACTACAGCGTCAATTTCCTGCCAACAGTAAAAATTGGTGTGGCGATTGCTGATGACCAACTCGATGAAGGGATCGATATCGTCATTAAGGCGGCTTCCACCGGGCAATTTGGCGACGGCAAAATCTTCGTCGCTgtcttg                                                                                                                                                                                                           >  2:53202/1‑246 (MQ=255)                                       cGTTCGTGAAGCGTTAGCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAAcgcg                                                                                                                                                                                                    <  2:89625/251‑1 (MQ=255)                                                     tATCTTCCATTGGTATTCAGGGCCTTACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCattcgtattcg                                                                                                                                                                                       <  1:321446/250‑1 (MQ=255)                                                                ggTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAg                                                                                                                                                                            <  1:284189/250‑1 (MQ=255)                                                                          gCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTTAATTTCCTGCCTAAAGTAAAAATTGATGTGGCGAGTGCTGATGACCAACTCGATGAAGTGCTCGATAACGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCAGTCGTATTCGTACCGGCGAAGCCGACGAAgcg                                                                                                                                                                 <  1:92884/251‑1 (MQ=255)                                                                          gCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAgcg                                                                                                                                                                 <  1:196463/251‑1 (MQ=255)                                                                              gACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGCCGGAATACAGCGTCAATTTCCTGCCAAACGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGCTGAAGTGATCGATCTCCTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAACATCTTCGTCGCTGACTTGCCACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGgcgc                                                                                                                                                             >  1:312542/1‑251 (MQ=255)                                                                              gACCGTCACCGAAGTGAAAGGTTGCGGGAGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAgtggct                                                                                                                                                              <  2:387736/250‑2 (MQ=255)                                                                                     aCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGACTACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGAGGCGATTGCTGATGACCAACTCGCTGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTCTTCGTACCGGCGACGCCGACGCAGCGGCGCTGTAAtt                                                                                                                                                      >  2:25042/1‑250 (MQ=255)                                                                                     aCCGAAGTGAAAGGTTTCGGGAGTAAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCCAAAGTAAAAATTGCTGTGGCGATTGCTGATGACCAACTCGATGCAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGAAGGCAAAATCTTCGTCGCTGAATTGCACCGCATCATTCGTATTCGTACCGGCGAAGACGACAAAGCGGCGCTGTAAtc                                                                                                                                                      >  2:273240/1‑251 (MQ=255)                                                                                                aGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGcacaca                                                                                                                                           <  1:277201/251‑1 (MQ=255)                                                                                                                                 gTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGAt                                                                                                          <  1:400361/251‑1 (MQ=255)                                                                                                                                     cGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATaaa                                                                                                       >  1:80816/1‑250 (MQ=255)                                                                                                                                            cGGAATACAGCGTCAATTTCCGGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACCCGATGAAGTGAGCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGgc                                                                                               <  1:428515/251‑1 (MQ=255)                                                                                                                                              gAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGgctt                                                                                             <  1:67675/251‑1 (MQ=255)                                                                                                                                              gAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTACTCTCTGGCACACAGCAACCGGAACGAAAAATGAAGATAGCGAAGATAAAAACAGGgctt                                                                                             >  2:306595/1‑251 (MQ=255)                                                                                                                                               aaTACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGgcttg                                                                                            <  2:178398/251‑1 (MQ=255)                                                                                                                                                   aaGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACAACGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTTAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTTATCTCTGGCACACAGCAACAGGAACGAAAAAAGAAGATAGCGGCGATAAAAAATGGGCTTGCTTc                                                                                        >  2:115194/2‑251 (MQ=255)                                                                                                                                                                   ccAAAAGTAAACATTGATGTGGCGATTGCTGATGACCACCTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAAAGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTTATTAACTGGCGATGCTTCCg                                                                        >  1:188347/1‑251 (MQ=255)                                                                                                                                                                   ccAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTAAGTAAGGCGGCTTACACCGGGAAAATGGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACAGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCATCAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCg                                                                        <  2:173871/251‑1 (MQ=255)                                                                                                                                                                       aaGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGAc                                                                    <  1:161665/251‑1 (MQ=255)                                                                                                                                                                         gTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTg                                                                  <  1:58794/251‑1 (MQ=255)                                                                                                                                                                                   aTGTGGCGATTGCTGATGACCAACTCGAGGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTg                                                        <  2:303693/251‑1 (MQ=255)                                                                                                                                                                                                 gatgaCCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACAGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGAATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGATTGCTTCACTGGCGATGCTTCCGGGACTGGCAATGGCTGCACCTGCGGTGGcc                                          >  2:459385/1‑251 (MQ=255)                                                                                                                                                                                                               tGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACa                             >  1:260663/1‑250 (MQ=255)                                                                                                                                                                                                                 aaGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGCAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGACAAATGAAGATAGCGACGCTAAAACCTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTTGCCGGTaacacagcccatg                          >  2:404189/1‑240 (MQ=255)                                                                                                                                                                                                                  aGTGATCGAAATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCTACGGCAAAATCTTCGTCGCTGAATTGCAACTCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCCCCTGCGGTGGCCGATAAAGCCGACAATGc                         <  2:308304/251‑1 (MQ=255)                                                                                                                                                                                                                   gTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTAGTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCg                        <  2:13975/251‑1 (MQ=255)                                                                                                                                                                                                                    tGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGt                       >  1:426080/1‑251 (MQ=255)                                                                                                                                                                                                                                  aGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGt         >  1:383287/1‑251 (MQ=255)                                                                                                                                                                                                                                         cggcTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTgcgc  >  1:187230/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              CTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGC  >  NC_000913/472604‑473086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑