Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,170	T→C	S262P (TCG→CCG)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,170	0	T	C	100.0%	146.0 / NA	46	S262P (TCG→CCG)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/27);  total (19/27)

ACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGG  >  NC_000913/70923‑71411                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  aCGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGACGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATTTTGGTTTTGACGATCAACTCTATTTCCcgc                                                                                                                                                                                                                                                >  2:481718/1‑251 (MQ=255)  cgcgAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCcgcg                                                                                                                                                                                                                                               <  1:345361/251‑1 (MQ=255)      aGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTTACGATCAACTCTATTTCCcgcgcgt                                                                                                                                                                                                                                            >  2:275339/1‑247 (MQ=255)        gCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTAtt                                                                                                                                                                                                                                         >  1:15317/1‑251 (MQ=255)           tGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTAttt                                                                                                                                                                                                                                        <  1:254673/249‑1 (MQ=255)              cAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTaaaa                                                                                                                                                                                                                                    >  1:373740/1‑250 (MQ=255)                  aCATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGc                                                                                                                                                                                                                               >  1:94787/1‑251 (MQ=255)                        acGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCACCGCATTAGTCAGGCGAAGCTGCTTTTGTGCACTACCCGGATGCCTATCGCCACCGTCGGCCGCTATGTTGGTTTTGAAGATCAACTCTATTTCCAGCGAGTATTTAAAAAAAGCACCgtg                                                                                                                                                                                                                         >  2:89510/2‑249 (MQ=255)                             cACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCa                                                                                                                                                                                                                     <  1:59371/250‑1 (MQ=255)                                          gCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttt                                                                                                                                                                                                       <  1:282324/251‑1 (MQ=255)                                               tttGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCGTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTg                                                                                                                                                                                                   <  1:278017/250‑1 (MQ=255)                                                             cGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa                                                                                                                                                                                    <  1:275339/251‑1 (MQ=255)                                                                      gccttgttGCTTGTCGCCGTCGCGTCGGTCACAACTTTTCCGCCAGCAGTTATGGATTAGCGTCTTAAGCTTGCTCGAGGACCAACGCATTAGTCAGGCGTAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATGTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTTTGAAGAAAAAGTGAatg                                                                                                                                                                           <  2:202554/245‑1 (MQ=255)                                                                      gCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatg                                                                                                                                                                           >  2:236921/1‑251 (MQ=255)                                                                      gCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatg                                                                                                                                                                           <  1:459355/251‑1 (MQ=255)                                                                        aTGTATGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGCGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATAAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat                                                                                                                                                                         >  2:258193/1‑251 (MQ=255)                                                                                ttggcGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGCTGCCTATCGCCACCGTCGGTCGCCATGTTGGTTTTGCCGATCAACTCTATTTCCCGCGAGTATTTAAAACATGCACCGGGGCCCGCCCGAGCGAGGTTCGGGCCGGATGTGTAGACAAAGTGaaagctggtgccat                                                                                                                                                                 >  2:75744/5‑239 (MQ=255)                                                                                 tGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTACGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCTGGGCCAGCCCGAGCGTGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc                                                                                                                                                                >  2:383222/1‑251 (MQ=255)                                                                                  gTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc                                                                                                                                                                <  1:223972/250‑1 (MQ=255)                                                                                   tCGCCGTCGCGTCTGTCACTTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAACTGCTTTTGAGCACTACCCGGCTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCAAGCCCGAGAGAGTTTAGTGACGGTTGTGAAGAAAAAGTGAATGATGTAGCCGtaac                                                                                                                                                              >  2:119720/1‑248 (MQ=255)                                                                                    cGCCGTCGCGTGTGTCTCATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGGCGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATTTATCCGTCAAg                                                                                                                                                             <  2:33347/251‑1 (MQ=255)                                                                                             gTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa                                                                                                                                                    <  2:78841/251‑1 (MQ=255)                                                                                             gTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa                                                                                                                                                     >  1:431537/1‑250 (MQ=255)                                                                                             gTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATCAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGGCATaa                                                                                                                                                    <  1:90479/251‑1 (MQ=255)                                                                                                          tttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGaa                                                                                                                                         <  1:110229/249‑1 (MQ=255)                                                                                                             ccGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAg                                                                                                                                     <  2:411025/250‑1 (MQ=255)                                                                                                              cGCCAGCAGTTAGGGAGTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGGCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGGGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGAc                                                                                                                                   <  2:223488/251‑1 (MQ=255)                                                                                                                 cgacagTTAGGGATTAGCGTCTTAAGCTGGCTCGAGGACCAACGCATTAGTGAGGCTAAGCTGCTTGTGAGCACTACCCCTTGGCCTATCGCCACCGCCGGAGGCAATGTTGGTTTTGACGATCAACCCTATTTCCCGCGAGTAGTTAAAAAATGCACCGTGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGTGGTCAAGGTGTCATAATTGGTACCGAATCAGGCAAt                                                                                                                                <  2:429943/248‑1 (MQ=255)                                                                                                                                                gcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCAACGGCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGc                                                                                                 <  2:51911/251‑1 (MQ=255)                                                                                                                                                       ccAACGTATTAGTCAGGCGAAGCTGATTTTTAGCTCGACCCGGATGCCGTTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATGTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATcc                                                                                          <  2:312696/251‑1 (MQ=255)                                                                                                                                                       ccAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCTCTACCCGGATGCCGATCGCCACCGTCGGTCGCAATGTTGGTTGTGACGAGCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTGGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATcc                                                                                          <  2:223162/251‑1 (MQ=255)                                                                                                                                                             cATTAGTCAGGCGAAGCTGCTTTTGAGCACTGCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCtt                                                                                    <  1:388016/251‑1 (MQ=255)                                                                                                                                                                       gCGAAGCTGATTTTGAGCACTACCCGGATGCCTATCGCAACCGTCGGTCGCAATGTTGATTTTGACGCTCAACTCTATTTCCCGCGAGTATTTAAAAAATGCCACGGGGCCAGCCCGAGCGAGTTTAGTGCCGGATGTGAAGAAAAAGTGCATGATGTATCCGTCAAGTTGTCATAATTGGTAACGAATCAGACACTTGACAGCTTGGAGGAGTCTCGTAGTGTTTGCAGAATCCCTGATTCGTCCAtttt                                                                          >  2:121108/1‑250 (MQ=255)                                                                                                                                                                           aGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCg                                                                                  <  2:127153/239‑1 (MQ=255)                                                                                                                                                                           aGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCg                                                                                  >  1:127153/1‑239 (MQ=255)                                                                                                                                                                                   ttGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGGTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATGTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGTCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACAtt                                                               <  1:202035/250‑1 (MQ=255)                                                                                                                                                                                     gAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATg                                                            <  1:168758/251‑1 (MQ=255)                                                                                                                                                                                        cACTACACGGATGCCTATCGCCACAGTCGGTCGCAATGTTGGCTTTGACGATCACCTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGAGCCGGTTGTGAAGAAAAAGAGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGACTCAGACAATTGACGGCTTGACGGGGTAGAATGGGGTTGGCAGAATCCCTGCTTCGTACATTTGACAGGCACCTGATGCa                                                          >  2:432286/1‑250 (MQ=255)                                                                                                                                                                                                 gttGCCTATCGCCACCGTCGGTCGCAATGTTGGGTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTCGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTg                                                <  2:260971/249‑1 (MQ=255)                                                                                                                                                                                                        aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAacac                                          <  1:236921/250‑1 (MQ=255)                                                                                                                                                                                                        aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACt                                         >  2:61979/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGATCAACTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATg         >  1:295790/1‑251 (MQ=255)                                                                                                                                                                                                                                        agATCAACTGTATTTCCCGCGAGTATTTAAAAAATGGACCGGGGCCGGGCCGAGCGAGTTTCGTGGCGGTGGTGAAGAAAAAGTGAATGAGGAAGCCGTCAAGGTGTCATAATTGGTAACGAATCAGACAAGTGACGGCGTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTGATTACCCAAGCCACCGTGTATTCATTGATg         <  2:221261/250‑1 (MQ=255)                                                                                                                                                                                                                                             aaCTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTgg                                               <  1:81712/208‑1 (MQ=255)                                                                                                                                                                                                                                             aaCTCTATTTCCCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTgg                                               >  2:81712/1‑208 (MQ=255)                                                                                                                                                                                                                                               ctctATTTCCCGCGAGTATTTAAAAAATGCACCGAGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGGAGCCGTCAAGCTGTCCTAATTGGTAACGAATCAGCCAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCATCGTCCATTTGACAGGCACATTTTGCAAGCATTGCTGGAACAATTTATTACCCAATCCTCCGTGTATTCATTGATGGCggggg  >  2:379989/1‑248 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  ACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGG  >  NC_000913/70923‑71411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑