Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	47,099	G→A	Q431Q (CAG→CAA)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	47,099	0	G	A	100.0%	91.7 / NA	32	Q431Q (CAG→CAA)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/15);  total (17/15)

GTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAGACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCG  >  NC_000913/46861‑47336                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              gTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGCGCCTCGAGGGGTGTCCCTGGCGCAAACCAGCAatttg                                                                                                                                                                                                                                   >  2:420858/1‑248 (MQ=255)                       tAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATgg                                                                                                                                                                                                             >  1:354797/1‑251 (MQ=255)                        aaTGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATggg                                                                                                                                                                                                            <  1:132070/251‑1 (MQ=255)                                   cccGACAGATATCCGCGTCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTc                                                                                                                                                                                                 <  1:458736/251‑1 (MQ=255)                                   cccGACAGAAATCCGCGCCTCTGCCGTAGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGtt                                                                                                                                                                                                  <  1:260387/250‑1 (MQ=255)                                      gACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGCTGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTTCGTAACGTGTGCCACGGAGACTCGAGGGATGTCACTGGCGCCAACCAGCAATATGACGAACCGCGGGCAGGGACGGGGGTAAATTGTTCAgg                                                                                                                                                                                              >  2:169826/1‑250 (MQ=255)                                             atCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTtctct                                                                                                                                                                                       >  1:49761/1‑251 (MQ=255)                                                                        aTGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGTTGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGAAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACtat                                                                                                                                                            >  1:41321/1‑251 (MQ=255)                                                                         tGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACtat                                                                                                                                                            >  2:237765/1‑250 (MQ=255)                                                                                aagccGTATTGGCACCATTGTTTCGACCTGGGCACGGCCGATCTTTATCAAACATTACGGGATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGATGTTTGGCTTGTTGATTTCCCTAGCGTTTACCCCGGGGACTCGGGGGATGTCACTGGAGCAAACCAGCAAGATGACGATCCGCGGGCAGAGAGTGGGGTAAATTGTTCAGATTTCTCTCTTTACTGACTCAATAATATTGACTATAAgacg                                                                                                                                                      >  1:296652/5‑246 (MQ=255)                                                                                     gTATTGGCACCATTGTTTCGACCGGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGGTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAat                                                                                                                                               <  2:289206/251‑1 (MQ=255)                                                                                              ccATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACt                                                                                                                                       <  1:418847/250‑1 (MQ=255)                                                                                                        gACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGCTTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTgg                                                                                                                            >  2:82365/1‑251 (MQ=255)                                                                                                                     ccGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGCTCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTAAACTTTGTGGGAAAACAAAGGCg                                                                                                               >  2:163011/1‑251 (MQ=255)                                                                                                                         tCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCGGAGAATGGGGTAAATTGTTCAGCTTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGAGAATATATGACTACACTTTGTGGGAAAACAAAGGCgaaa                                                                                                            >  2:470883/1‑247 (MQ=255)                                                                                                                              aTCAATAATTACTGTATTAGTAACACGATGCTAATGTGGGCGGGTATCTCGCTGTTGGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCTCAAACCAGCAATATGACGATCCGCGGGCAGAGAATTGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCAcgc                                                                                                      <  2:418264/251‑1 (MQ=255)                                                                                                                                cAATAATTACGGGATCAGTAACACGATGCTAATGGGGGCTGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCAcgcg                                                                                                     <  1:126571/250‑1 (MQ=255)                                                                                                                                              tCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAACGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTCAATTGTTCAGATTTATCTCTTTTCTGAATCCATATTATTGACTATAAGCCGCGGGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGAttt                                                                                      >  2:77593/1‑250 (MQ=255)                                                                                                                                                 gTAACACGAGGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTta                                                                                   <  2:136036/251‑1 (MQ=255)                                                                                                                                                        gctGCTAATGGGGGCGAGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAACGTTTGCCCCGGAGACTCGGGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGGAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATCTATGACTACACATTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGAGTCTTATAAttt                                                                             >  2:269131/3‑250 (MQ=255)                                                                                                                                                        gATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAAtttt                                                                            >  1:384991/1‑250 (MQ=255)                                                                                                                                                                                     ttGGCTTGTTGATTTCCGTAGCGGTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCAt                                                <  1:83036/250‑1 (MQ=255)                                                                                                                                                                                              tgATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATACTTTATGCGCATCCGTATCCGCATCATTCCCATGCGATTAAAc                                      >  1:95346/1‑251 (MQ=255)                                                                                                                                                                                               gATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTaca                                                                                                                                    >  2:165318/1‑156 (MQ=255)                                                                                                                                                                                               gATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTaca                                                                                                                                    <  1:165318/156‑1 (MQ=255)                                                                                                                                                                                                              tGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGc                      >  1:1190/1‑251 (MQ=255)                                                                                                                                                                                                                  ccGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAgg                  <  1:37219/251‑1 (MQ=255)                                                                                                                                                                                                                       gaCTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCt             <  2:354038/251‑1 (MQ=255)                                                                                                                                                                                                                              gggAGGTCACTGGCGCAAATCAGCAATATTCCGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCACTCTTTTCTGAAGCAATATTATTGACTATAAGCCGCGTTAATATATGACTACACTTTGTGGGAAAACAAAGTCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGCTGCTTGAACAGGCAAGGCCGCTGTAAGGc      <  2:155016/251‑1 (MQ=255)                                                                                                                                                                                                                                 aTGTCCCTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGCTGCTTGAACAGGCAAGGACGCTGGAAGGCGTc   <  2:354797/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGTAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCg  >  2:125862/1‑251 (MQ=255)                                                                                                                                                                                                                                   gTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCg  <  1:420858/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              GTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAGACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCG  >  NC_000913/46861‑47336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑