Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,790	A→G	L328L (CTA→CTG)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,790	0	A	G	100.0%	73.2 / NA	30	L328L (CTA→CTG)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/20);  total (10/20)

CCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTAT‑CCTAAT‑GAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGAT  >  NC_000913/46564‑47014                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   catggcaggggttCCCAAGGTTCGTCATTTACACCTTTGGCCCGCAAATCGTGGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCGCTGGGGAACGGGGTGATTAGCCTGTTCTTTAGGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATctggctg                                                                                                                                                                                                            <  2:350564/239‑1 (MQ=255)                 aaTGTTCGCCATTTACACCTTTGGCCCGCAAATCGTGGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTAGGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATGTGGGGATCTGGCTGGTAGTGATGGCCTTTg                                                                                                                                                                                            <  2:390676/251‑1 (MQ=255)                   tGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGCTTTGTTGGGATTGGGTGTTGGCCAAAACGCGGCGCTGGGGACCGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGCGGCTGGGGTTAAACACTGCCGGACGGAGTCCATTGTTGATTGGCAGCTTTGCCATGATGCCGCTGCCGCGGGCGGTTTTGGGGGTGGTCCCGGGTGTGGGGAttggggtggggtgggtgggctttgtg                                                                                                                                                                                          >  2:37219/1‑226 (MQ=255)                       cGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGgtgt                                                                                                                                                                                      <  1:157666/251‑1 (MQ=255)                             ttacacctgtgcggcGCAAATCGTTGGTTTGTTGGGATTGGGTGTTAGCAATAACGGGGCGTTGGGGAGCGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGGATGCCGCCGATGCTCTGGTTAACCACTGGCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGATGGCGGTTTTGTGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTGGCGGTGTATGcc                                                                                                                                                                                 <  1:475279/236‑1 (MQ=255)                               acacCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTGATGCTCGGCTGTATTCCTCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCttt                                                                                                                                                                              <  1:390018/251‑1 (MQ=255)                                         cccGCAAATTGTCGGATTGGTGGGATTGGGGGTTGGCAAAAACGCGGCGCTGGGGGACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTATGCCATGATGACGCTGGCGCTGGCGGATTTGGGGGTGAGCCCGGGTATGGGGACCTGGTGGGGGGGGAGTGCCTTTGTGGTGTATGCCtttttgttctgcg                                                                                                                                                                    >  2:83036/1‑243 (MQ=255)                                                   gTTGGTTTGTTGGGATTGTGTGTTGGCAAAAACGCGTGGCTGGGGAAGGTGGTGAGTAGCCTGTTCTTTATTCTCGGCTGTATTCCGCCGATGCGTTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCTGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTaa                                                                                                                                                          <  2:388897/251‑1 (MQ=255)                                                     tgggttgttgGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATgg                                                                                                                                                                                                  <  1:368741/205‑1 (MQ=255)                                                     tGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTTGGGCTGATCCCGGGTATGGGGGTCTGGCTGGTGGTGATGGGCTTTTCGGTGTAAGCCTTTTTTTCTGGCGGGGCGGGGAAtt                                                                                                                                                        >  1:136036/1‑251 (MQ=255)                                                       gTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTg                                                                                                                                                      <  1:403237/251‑1 (MQ=255)                                                                         ttGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGGTATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGGGTATGCCTTTTTTTCTGGCGGGCCGGGGAATTTGCAGTGGCGCTAT‑CCTAAt                                                                                                                                   >  1:354038/1‑251 (MQ=255)                                                                          tGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTAT‑CCTAAt                                                                                                                                   <  1:333785/250‑1 (MQ=255)                                                                                                     ggtgATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTAT‑CCTAAT‑GAACTCTTCCCGACAGATATCCGCGCct                                                                                                      <  1:126528/251‑1 (MQ=255)                                                                                                      gtgATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGGTCCCCGATATGGGGATTTGGCTGGGAGTGGTGGGCTTTGAGGTGGATGCCTTTTTCTCTTGCGGGGCGGGGAAATTGCCGTGGGT‑TATTCCCAAT‑GAAATATTCCCGACAGataacacgccccc                                                                                                     >  1:374880/1‑241 (MQ=255)                                                                                                        gATTGGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTAT‑CCTAAT‑GAACTCTTCCCGACAGATATCCGCGCCTCTg                                                                                                   <  2:182811/251‑1 (MQ=255)                                                                                                                    cTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTTGGGCTGATCCCg                                                                                                                                                                                                                              >  1:76843/1‑118 (MQ=255)                                                                                                                    cTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCg                                                                                                                                                                                                                              <  2:76843/118‑1 (MQ=255)                                                                                                                              ggCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTCATGGCCTTTGCGGTGTATGCCTTTTTTTTTGGCGGGGCGGGTAATTTTCAGGGGCCCTAT‑TCTAAA‑TAACTCCTCCCGACAGATATCCGCGGCTCTTCCGTggggggtattatggcctt                                                                             >  1:348454/1‑236 (MQ=255)                                                                                                                                  gTATTCCGCCGATGCTGTGGTTAAACACTGCAGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTTGGGCGGATTCCGGGTATGGGGGTCTGGCTGGTAGTGATGGCCTTTGCGGGGTATGCCTTTTTCTCTGGCGGACCGGGGAATTTGCAGTGGCCCTTT‑TCTAAT‑GAACTCTTCCCGGCCGATATCCCCGCCCCTGCCGGGGGCGTGCTTTTTTCCTTAAgc                                                                         >  1:418264/1‑250 (MQ=255)                                                                                                                                    aTTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTAT‑CCTAAT‑GAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAgccg                                                                       <  1:449050/251‑4 (MQ=255)                                                                                                                                    aTTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATGGTTTATTGGCAGCTTTGCCATGATGACGCGGGCGCTGGCGGTTTTGGGGCTGATCCGGGATATGGGGATCTGGTTGGTAGTTATGGCTTTTGCGGTGTATGCCTTGTTCTCTGGCGGTCCGTGTAATTTTCAGTGTCTCTAT‑CCTAAT‑GAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAgccg                                                                       <  1:27094/251‑4 (MQ=255)                                                                                                                                              tGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTTGGGCTGGTCCCGGGTATGGGGATCTGGCTGGGGGTGAAGGCCTTTGCGGGGTATGCCTTTTTTTCTGTCGGGGCGGGCGATTTTCAGTGGTTCCTT‑CCCAATTAAATTCTTCC‑GACAGATATCCGGCCCTCTTCCGTGGGGGTGTTTATTTCCTTaacccggattggtacc                                                             >  2:202501/1‑237 (MQ=255)                                                                                                                                                      gtAAACACTGCCGGACGGCGTACATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTAT‑CCTAAT‑GAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGAGTATGTCCTTAAGCCGTATTGGCACCATTGTTTc                                                     <  1:379144/250‑1 (MQ=255)                                                                                                                                                                          tCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTTGGGCCGGTCCCGGATATGGGGATCTGGCTGGGAGGGATGGCCTTTGCGGGGTATGCCTTTTTCTCTGGCGGGCCGGGGCAATTGCAGTGGCTCTCT‑CCTAAT‑GAACTCTTCCCCCCAGATATCCGCGCCCCCGCCCGGGGCGTGCTTATGTCCTTTAGCCGTATTGGCACCATTATTTTGACCCGGGCACTGCCGagct                                 >  1:289206/1‑248 (MQ=255)                                                                                                                                                                               tgttgATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGAGCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTAT‑CCTAAT‑GAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTAt                             <  1:375873/250‑1 (MQ=255)                                                                                                                                                                                               tGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTAT‑CCTAAT‑GAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATc             <  1:457758/250‑1 (MQ=255)                                                                                                                                                                                                 ccATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTAT‑CCTAAT‑GAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTAt              <  1:13653/247‑1 (MQ=255)                                                                                                                                                                                                       ggaCGCGGGCGTTGGCGGGTTTGGGGCTGATCCCGGATTTGGGGATTGGGCTGGGAGGGATGGTGTTTGCGGTGTATGACTTTTTCTTGGGCGGGCCGGGTAAGTTGCAGTGGCGCTAT‑CCTAAT‑GAACTCTTCCCGACAGAGATCCGCGCCTCGGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACg    <  2:266714/250‑1 (MQ=255)                                                                                                                                                                                                         aCGCTTGCGCTGTCGGGTTTGGGGCTGATCCCGGATATGGGGATCTGGTTGGTATTGTTGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTAT‑CCTAAT‑GAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGAt  <  1:131400/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   CCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTAT‑CCTAAT‑GAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGAT  >  NC_000913/46564‑47014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑