Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 27,104 G→A Q276Q (CAG→CAA)  ispH → 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091327,1040GA94.6% 159.5 / ‑1.9 57Q276Q (CAG→CAA) ispH4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (19/34);  total (21/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

ATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCG  >  NC_000913/26857‑27344
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
aTGACTTCTGCTACGCGACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAAt                                                                                                                                                                                                                                               <  2:466742/251‑1 (MQ=255)
  gACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATAGGCTCCGGAAATTCTGGTGCAAAAtgt                                                                                                                                                                                                                                             >  2:337630/1‑251 (MQ=255)
        tGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAACATCCTCCAACTCCAACCGTCTGGCGGAGCGGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGGGGGTGAAAGAGGTTAAATGCGTCGGCGTGACAGCGGGCGCATCGGCTCCGGATATTCTGGTGCCAAAtgtgtgggc                                                                                                                                                                                                                                       >  2:175644/1‑246 (MQ=255)
             cGCCACGACTAACCGTCAGGAAGCGGTACGCTCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTGCGAAAAACTCCTCCAACTCCAACCGTCTGTCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGtt                                                                                                                                                                                                                                  <  1:285569/251‑1 (MQ=255)
               ccACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTg                                                                                                                                                                                                                                >  2:227772/1‑251 (MQ=255)
                        acagTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAACCGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAGGGGTGGGTGAAAGGGGTTAAGTGCGTCGGCGTGCCGGCGGGCGCATGGGCCCCGGATATTCTGGGGCGGAAGGGGGTGGCAGGTTTGCGGCGGCTg                                                                                                                                                                                                                       >  2:237504/4‑251 (MQ=255)
                                 aaGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCggtggt                                                                                                                                                                                                              <  2:379024/251‑1 (MQ=255)
                                  aGCGTTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGgtggtg                                                                                                                                                                                                             <  1:222308/251‑1 (MQ=255)
                                    cGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGaa                                                                                                                                                                                                           <  1:107045/251‑1 (MQ=255)
                                       tACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGTTTCGACAAACTCCTCCAACTCCAACCGGCTGGCGGTGCTGGCCCCGCGTCTGGGCCAACGCGCGTTTTTGATTGGAGATGCGAAAGATATCCAGGAAGAGTGGGTGAACGAGGTTAAATGCGTCCGCGTGACTGCTGGCGCCACGGCTCCGGCTATTCTGGTGCCAAATGTGGTGGAACGTTTGCAACAGCAGGGCGGTGATGAAGcc                                                                                                                                                                                                        >  2:137590/1‑251 (MQ=255)
                                         cgcgCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGGGGTGAAGCCAt                                                                                                                                                                                                      >  1:280459/1‑251 (MQ=255)
                                           cgcCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACACCAACCGTCTGGCGGAGCTGGCACAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTc                                                                                                                                                                                                    >  1:227024/1‑251 (MQ=255)
                                             cccTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCg                                                                                                                                                                                                  <  1:73447/251‑1 (MQ=255)
                                                          ggCGGAAGTTGTGTGTGTGGCCGGTTCGAAAAACCCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGGATGGGCAAACGCGCGGTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGtt                                                                                                                                                                                     <  2:184557/251‑2 (MQ=255)
                                                                      gtTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACAtt                                                                                                                                                                          <  2:16220/250‑1 (MQ=255)
                                                                      gtTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGAATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCACCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGGGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTg                                                                                                                                                                         >  2:101237/1‑251 (MQ=255)
                                                                        tggtggTCGGTTCGAAAAACTCCTACAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGCGTGGGTGAAAGAGGTTAATTGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGATGCAAAATGTGGTGGCACGTTTGCAGAAGCAGGGCGGTGGTGAAGCCATTACGCTGGAAGGCCATgaagacaaagttgtt                                                                                                                                                                       >  2:176234/1‑241 (MQ=255)
                                                                        tgggggtCGGTTCGAAAAACTCCTCCGACCCCAACCTTCTGGCGGAGCTGGCCCAGCGTATGGGCAAGCGCGCGTTGTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGGTTGAAGCAGCTGGGCGGTTGTGAAGCCATTCCGCTGGAAGTCCGTTAAGAAAACATTGt                                                                                                                                                                        <  2:325957/246‑1 (MQ=255)
                                                                                     gAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGGGCCg                                                                                                                                                           >  2:481384/1‑250 (MQ=255)
                                                                                         aaCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAaga                                                                                                                                                      <  1:120628/251‑1 (MQ=255)
                                                                                                    ctccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCg                                                                                                                                           >  2:147743/1‑251 (MQ=255)
                                                                                                          ccGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTc                                                                                                                                     <  1:156037/251‑1 (MQ=255)
                                                                                                                gggggAGTTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTTGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCAGCGGCTCCGGATATTCTGTTGCAAAATGTGGTGGCACGTTTGCAGAAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCTATATTCGTg                                                                                                                                  <  1:247744/245‑1 (MQ=255)
                                                                                                                ggCGGAGCTGGCCCAGCGGATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGa                                                                                                                                 <  2:41687/249‑1 (MQ=255)
                                                                                                                         ggCCCAGCGGATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGAGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGATCTGCGTGTCGATATTCGTGAAGTCGATTaa                                                                                                                       <  1:375488/250‑1 (MQ=255)
                                                                                                                          gCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGt                                                                                                                     <  1:275136/251‑1 (MQ=255)
                                                                                                                             cAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCAt                                                                                                                  <  1:119095/251‑1 (MQ=255)
                                                                                                                                         aaaCGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGTCTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAg                                                                                                      <  1:416567/251‑1 (MQ=255)
                                                                                                                                                                  cGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTa                                                                             <  1:422743/251‑1 (MQ=255)
                                                                                                                                                                    aaaGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTAcc                                                                           >  1:353672/1‑251 (MQ=255)
                                                                                                                                                                        ataTCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAATAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCa                                                                       <  1:166229/249‑1 (MQ=255)
                                                                                                                                                                        ataTCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGa                                                                                              >  2:105037/3‑228 (MQ=255)
                                                                                                                                                                        ataTCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGa                                                                                              <  1:105037/226‑1 (MQ=255)
                                                                                                                                                                          aTCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCAtt                                                                     <  1:227772/251‑1 (MQ=255)
                                                                                                                                                                          aTCCAGGAAGAGGGGGGGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCTGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCAtt                                                                     <  2:50783/251‑1 (MQ=255)
                                                                                                                                                                              aGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGATCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGGGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGTTTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCAtttttt                                                                 >  2:392935/1‑251 (MQ=255)
                                                                                                                                                                               gtaagcgggggTGTAAGAGGTCTAATGCGTCGGCGGAACTGCGGGCGCACCGGCTCCGGATATTCTGGTGCAAAATGTGCTGGCACGGTTGCAGCAGCTGGGCGGTGGTGAACCCAGTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGCGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGGCGATTAAGTCATTAGCAGCCCAAGTGATGCGAAACTGCCGGTCTTGTTACCGGCAGTTTTTa                                                                <  2:112584/243‑1 (MQ=255)
                                                                                                                                                                               ggAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCAATAGCAGCCTACGTTATGCGAAAATGCCGGTCGTGTTACCGGCAttttttt                                                                >  2:153907/1‑250 (MQ=255)
                                                                                                                                                                                  agagagGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCAGGTGCAAAATGTGGAGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTACGTTATGCGAAAATGCCGGTCTTGTTACCGTCATTTTTTATgg                                                             >  2:375831/1‑251 (MQ=255)
                                                                                                                                                                                  agagTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAACTGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGTAGTCGATTCAGTCATTAGCAGCCTAAGTTATGCGATAATGCCGGTCTTGTTACCGGCATTTTTTCTgg                                                             >  2:95098/1‑251 (MQ=255)
                                                                                                                                                                                   gagTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGTCATTTTTTATGga                                                            <  1:95098/251‑1 (MQ=255)
                                                                                                                                                                                     gTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGgaga                                                          <  1:2683/251‑1 (MQ=255)
                                                                                                                                                                                         gggacagcgtttAAATGCGTCGGCGTGACTGCGGGCGACTCGTCTCCGGATATTCTGGGGCAAAAGGTGGTGGTACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCACTCCGCTTGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGGTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTAGGGAGAAAAc                                                      <  2:390823/241‑1 (MQ=255)
                                                                                                                                                                                           gAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACAt                                                    <  1:279977/251‑1 (MQ=255)
                                                                                                                                                                                             aaGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGc                                                  <  2:58353/251‑1 (MQ=255)
                                                                                                                                                                                             aaGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGAGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGc                                                  <  2:339770/251‑1 (MQ=255)
                                                                                                                                                                                                agtttAAATGCGTCTGCGGGACTGCGGGTGCATCGGCTCCGGATATTCGGGTGCAAAATGTGGTGGCACGGTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGGTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGtt                                               <  2:36467/248‑1 (MQ=255)
                                                                                                                                                                                                  gTTAAATGCGTCGGCGAGACTGCGGGCGCATCGGCTCCGGATATTATGGTGCACAATGGGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAGGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCCTTTTTTATGGAGACAACATGCAttt                                              >  2:74640/1‑250 (MQ=255)
                                                                                                                                                                                                     aaaTGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGCTTAAGGCATTAGCAGACTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGAGTTTACCt                                          >  2:66907/1‑251 (MQ=255)
                                                                                                                                                                                                       aTGCGTCGGCGTGCCTGCGGGCGCAGCGGCTCCGGATATTCTGGTGCAAAATGTGGGTGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCAATAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTAt                                        <  1:313774/251‑1 (MQ=255)
                                                                                                                                                                                                         gcgtcggctgggacgcgggagccacGGCTCAGGATAGTCTGGTGCAAAATGTGGTGGCACGTGTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACACTGTTGTCGAAGTGCCTAAAGATCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCTTAAGTTATGCGAAAATGCCGGTCTTGTGACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCt                                      <  2:110585/227‑1 (MQ=255)
                                                                                                                                                                                                          cGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCa                                                                                                                   >  1:36134/1‑173 (MQ=255)
                                                                                                                                                                                                          cGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCtt                                     <  2:72401/251‑1 (MQ=255)
                                                                                                                                                                                                          cGTCGGCGGGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCAGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCa                                                                                                                   <  2:36134/173‑1 (MQ=255)
                                                                                                                                                                                                                                    cTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACg           >  2:277357/1‑251 (MQ=255)
                                                                                                                                                                                                                                            atTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTc   <  1:441978/251‑1 (MQ=255)
                                                                                                                                                                                                                                              tCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTTAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATAGTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCg  <  1:389394/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
ATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCG  >  NC_000913/26857‑27344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑