Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 24,085 A→G Q565Q (CAA→CAG)  ileS → isoleucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091324,0850AG97.0% 292.7 / ‑1.9 101Q565Q (CAA→CAG) ileSisoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base G (38/59);  minor base T (1/2);  total (40/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

Only 100 of 101 total aligned reads displayed.

AGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAA  >  NC_000913/23839‑24328
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
agagCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCAcc                                                                                                                                                                                                                                                 <  1:299602/251‑1 (MQ=255)
 gagCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCGAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACcg                                                                                                                                                                                                                                                <  2:464702/251‑1 (MQ=255)
 gagCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACcg                                                                                                                                                                                                                                                <  1:233390/251‑1 (MQ=255)
   gatGCATCCGCGTACCCTCGAACTGTAGGAAGAAGTGGCAAAACGCGTTTAAGTTAACTGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACcgtg                                                                                                                                                                                                                                              <  1:287138/249‑3 (MQ=255)
      gCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGAAGTATGGTTTGGCTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTATGGCCAGCACcgtggtt                                                                                                                                                                                                                                           >  2:86077/1‑246 (MQ=255)
       cATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTGGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACcgtggttg                                                                                                                                                                                                                                          <  2:384567/251‑7 (MQ=255)
        aTCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGAGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTACGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGCCA‑TGTATCTGGAAGGTTCTGACCAGCACcgtggttgg                                                                                                                                                                                                                                         >  1:224604/1‑244 (MQ=255)
          ccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGtggttggt                                                                                                                                                                                                                                        <  1:205248/250‑1 (MQ=255)
                 cccTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCt                                                                                                                                                                                                                                <  1:363694/251‑1 (MQ=255)
                     cgAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTccc                                                                                                                                                                                                                            >  2:333697/2‑251 (MQ=255)
                      gAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCt                                                                                                                                                                                                                           <  2:150542/251‑1 (MQ=255)
                       aaCTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCt                                                                                                                                                                                                                           >  2:456419/1‑250 (MQ=255)
                        acggATGGAAGAAGGGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGCAGCTGACCAGTATGTGGAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGGTCTACCCACGCTTCTGTTGTTGTCGCGCGTCCGGCATTTGCCGGGAACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCCTCCCTat                                                                                                                                                                                                                         >  2:161011/4‑250 (MQ=255)
                           gATGGAAGAAGTGGCAAAACGCGTTTGAGTTAACGGCCCCCAGGCGTGGTTGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCCCCGTTGTTGGTTCATGTCTTCCCTAAt                                                                                                                                                                                                                        <  2:297164/249‑1 (MQ=255)
                            aTGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGACTTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAt                                                                                                                                                                                                                     >  2:74095/1‑251 (MQ=255)
                               gaagaaGTGGTACAACGCGTTGAAGTCAACGGCATCAAGGCGTGATGAGATCTCGATGCGCAAGAGATCCTCTGCGATGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCGGTTGTTGACTTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCATCACCGTGGTTGGTTCATGTCGTCCCTaagtatctc                                                                                                                                                                                                                  <  2:202036/251‑8 (MQ=255)
                                 agaagTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCa                                                                                                                                                                                                                <  2:77394/251‑1 (MQ=255)
                                   aagTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCAcc                                                                                                                                                                                                              <  1:198477/251‑1 (MQ=255)
                                      tGGCAAAACGCGTTGAAGTTAACGGCATCCATGAGTGGTGGGATCTCGATGCAAAAGAGATCCTCGGCGACGAATCTGACAAGTATGTGAACGTGACGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGG‑CACTGTATCTGGACGGTTCTGCACAGCCCCGTGGTTGGTTCATGCCTTCCCTAATAATCTCCACcgcg                                                                                                                                                                                                           >  2:268165/1‑251 (MQ=255)
                                                          aacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGt                                                                                                                                                                                        >  1:346015/4‑250 (MQ=255)
                                                              gCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGt                                                                                                                                                                                   >  1:125609/1‑251 (MQ=255)
                                                               cATCCAGGCGTGGTGGGAGCCCGATGCGAAAGATATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATCCTCTCGACGGATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTc                                                                                                                                                                                  <  2:224604/251‑1 (MQ=255)
                                                                  ccAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGTCGAAGCTGACCAGTATGTGAAAGTGCCTGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCGCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAg                                                                                                                                                                                <  2:40357/250‑1 (MQ=255)
                                                                    aGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGt                                                                                                                                                                              >  1:413836/1‑250 (MQ=255)
                                                                             gggATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCAc                                                                                                                                                                    <  2:276684/251‑1 (MQ=255)
                                                                              ggATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACg                                                                                                                                                                   <  1:311493/251‑1 (MQ=255)
                                                                                   gcGATGCGAAAGAGGGCCTCGGCGGTGAAGCTGACCAGTATGTTAAAGTGCCGGATACTCTCGTCGTATTGTGTGACTCCGGATCTTCCCACTCTTCGTTTGTGGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGGCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCTGTATCGTCAGGTACTGACCCACGGCtt                                                                                                                                                               <  2:431823/249‑1 (MQ=255)
                                                                                    cGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCttt                                                                                                                                                              <  2:242327/250‑1 (MQ=255)
                                                                                         cGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTg                                                                                                                                                        >  1:125602/1‑251 (MQ=255)
                                                                                             agagATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATg                                                                                                                                                    >  1:241828/1‑251 (MQ=255)
                                                                                             agagATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGAACTGACCCACGGCTTTACCGTGGATg                                                                                                                                                    >  1:205447/1‑251 (MQ=255)
                                                                                               agaTCCTCGGCGACGCAGCTGACCAGTATGTGCAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCGACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGTCA‑TGTCTCTGGAAGGTTCTGCCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCCTTACCGTGGATGGt                                                                                                                                                  >  2:18457/1‑251 (MQ=255)
                                                                                               agaTCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGt                                                                                                                                                  >  2:40061/1‑251 (MQ=255)
                                                                                               agaTCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGt                                                                                                                                                  >  1:25444/1‑251 (MQ=255)
                                                                                                gaTCCTCGGCGACGATGCTGACCAGTATGTGAAATTGCCGGATACTCTCGCCGTAGGGTTTGACTCCGGATCTACCCACTCTGCTGTTGTTGACGTGCGTCCGGAAATTGCCGGTTACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGGCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTATCGTGGATGGTc                                                                                                                                                 <  2:282799/251‑1 (MQ=255)
                                                                                                  tCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTc                                                                                                                                                 >  2:425456/1‑249 (MQ=255)
                                                                                                        gCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGc                                                                                                                                         <  1:408489/251‑1 (MQ=255)
                                                                                                         agacgaAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGAGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATAGTCAGGTACGGACCCACGGCTTTACCGGGGATGGTCacgggcgc                                                                                                                                         >  2:5231/2‑243 (MQ=255)
                                                                                                            cgaAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCa                                                                                                                                        >  2:306390/1‑248 (MQ=255)
                                                                                                                 cTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATg                                                                                                                                                                                                                                   <  2:320078/152‑1 (MQ=255)
                                                                                                                 cTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATg                                                                                                                                                                                                                                   >  1:320078/1‑152 (MQ=255)
                                                                                                                            gTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGGATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGATCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCgg                                                                                                                     >  1:286386/1‑251 (MQ=255)
                                                                                                                              gAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGGTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCa                                                                                                                   <  1:432619/251‑1 (MQ=255)
                                                                                                                                aagtgccgtatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGGTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaac                                                                                                                 <  2:315056/231‑2 (MQ=255)
                                                                                                                                   tgccggatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACAcc                                                                                                              >  1:278062/18‑251 (MQ=255)
                                                                                                                                     ccggatactctcgacgTATGGTTTGACTCCGGATCTACCCTCTCTTCTGTTGTTGACGTGCGGCCGGAAGTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGt                                                                                                            <  1:74095/236‑1 (MQ=255)
                                                                                                                                     ccggatactctcgacgTATGGTTTGACGCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGt                                                                                                            <  2:413836/236‑1 (MQ=255)
                                                                                                                                        gatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGTACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCCCCGCGATGAAAGGCCAAGCTCCGTATCGTCAGTTACTGACCCACGCCTTTACAGTGGATGGTCAGGTCCGCCAGCTGTCTAAATCCATCGGCacccccgtttc                                                                                                         >  2:91/13‑241 (MQ=255)
                                                                                                                                        gatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTc                                                                                                         <  1:456419/239‑1 (MQ=255)
                                                                                                                                                cgacgTATGGTTTGACTCCGGATCTCCCCACTCTTCTGTTGTTGACGTGCGGCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCCCCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAg                                                                                                  <  2:383009/246‑1 (MQ=255)
                                                                                                                                                  gcggaTGGTTTGACTCCGGATCTCCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACCCCGTTTCGCCGCAGGAt                                                                                               <  2:396684/247‑1 (MQ=255)
                                                                                                                                                      aTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGtga                                                                                           <  1:132675/251‑1 (MQ=255)
                                                                                                                                                         gTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGCTGtgatg                                                                                         >  2:294695/1‑250 (MQ=255)
                                                                                                                                                         gTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGtgatg                                                                                         >  1:134802/1‑250 (MQ=255)
                                                                                                                                                          tttGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTc                                                                                                         <  2:150355/233‑1 (MQ=255)
                                                                                                                                                          tttGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTc                                                                                                         >  1:150355/1‑233 (MQ=255)
                                                                                                                                                           ttGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTAGCTGGAAGGTTCTGCCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTGCGCCGCAGGATGTGATGaa                                                                                       <  1:172941/250‑1 (MQ=255)
                                                                                                                                                           tgtaCTCCTGATCTTCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGGATCTGGAAGGTTCTGACCAGCACCGTGGTTTGTTCATGTCTGCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGaa                                                                                       <  2:190197/247‑1 (MQ=255)
                                                                                                                                                                cccGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGGCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACt                                                                                 <  2:245895/250‑1 (MQ=255)
                                                                                                                                                                cccGGATCTACCCACTCTTCTGTTGGTGACGTGCGTCCGGAATTTGCCGGTCCCGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCCCCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACt                                                                                 <  2:136474/250‑1 (MQ=255)
                                                                                                                                                                  cGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTgg                                                                               <  1:232415/251‑1 (MQ=255)
                                                                                                                                                                  cGGATCTACCCACTCTTCGGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTgg                                                                               <  2:471822/251‑1 (MQ=255)
                                                                                                                                                                    gATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGgc                                                                             >  1:359691/1‑251 (MQ=255)
                                                                                                                                                                         aCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCCCCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGgc                                                                             <  1:367436/246‑1 (MQ=255)
                                                                                                                                                                         aCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGgc                                                                             <  1:211550/246‑1 (MQ=255)
                                                                                                                                                                         aCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCgg                                                                         <  1:40061/250‑1 (MQ=255)
                                                                                                                                                                         aCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGa                                                                        <  1:359122/251‑1 (MQ=255)
                                                                                                                                                                                  tctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGc                                                                <  1:316857/250‑1 (MQ=255)
                                                                                                                                                                                           gACGTGCGTCCGGAATTTGCCGGTCCCGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTgtggg                                                       <  1:221318/250‑1 (MQ=255)
                                                                                                                                                                                             cGTGCTTCCGGTATTTGCCGGTCACGCATCGGACA‑TGTATCTGGAAGGTTCTGACCAGCTCTGTGGTTGGTTCATGTCTGCCCTAATGATCTCCACCGCGATGAAAGGCTAAGCGCCGTATCGTCAGTTACTGACCCACGTCTTTACCGTGGATGGTCAGGGCCTCAAGATGTTTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTgtgggtgg                                                    <  2:310916/251‑1 (MQ=255)
                                                                                                                                                                                             cGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCATCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTgtgggggg                                                    >  2:377081/1‑248 (MQ=255)
                                                                                                                                                                                             cGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCa                                                                                                                                        >  2:238731/1‑167 (MQ=255)
                                                                                                                                                                                             cGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCa                                                                                                                                        <  1:238731/167‑1 (MQ=255)
                                                                                                                                                                                                  gTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCa                                               <  1:306141/251‑1 (MQ=255)
                                                                                                                                                                                                    ccGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGGTGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAAc                                             >  1:180282/1‑251 (MQ=255)
                                                                                                                                                                                                      ggAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCg                                           <  1:2811/251‑1 (MQ=255)
                                                                                                                                                                                                        aaTTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGATTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGAGGCATCAACCGAc                                         >  1:359760/1‑251 (MQ=255)
                                                                                                                                                                                                         aTTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCATCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACt                                        <  1:377081/251‑1 (MQ=255)
                                                                                                                                                                                                          tttGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACt                                        <  2:400542/250‑1 (MQ=255)
                                                                                                                                                                                                             gCCGGTCCCGCATCGGACA‑TGTATCTGGAAGGTACTGACCAGCACCGTGGTTGGTTCATGTCTGCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTaca                                     <  2:253088/250‑1 (MQ=255)
                                                                                                                                                                                                                   cACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAAc                                                                                      <  1:359622/195‑1 (MQ=255)
                                                                                                                                                                                                                   cACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAAc                                                                                      >  2:359622/1‑195 (MQ=255)
                                                                                                                                                                                                                      ccAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTATTGATCTCCACCGCGATGAAAGGCAAAGCGCCGTAACGTCAGGTTCTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGCCTAAATCAATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCTACTACACCGGTGAAAt                           <  2:72836/250‑1 (MQ=255)
                                                                                                                                                                                                                       cAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATg                          >  1:402618/1‑251 (MQ=255)
                                                                                                                                                                                                                        aGCGGACA‑TGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATgg                         >  1:122334/1‑251 (MQ=255)
                                                                                                                                                                                                                          acggaactGTAACTGGAAGGTTCCTACCAGCAACGTGGTTGGTTCATGTCTTTCCTATGGATTTCCACCGCGATGAAAGGCAAAGCGCCGTAACGGTAGTTACTGACCCACGGCTTTACCGTGGAGGTTCAGGGCCGCAAGCTGTCTAAATCCATCGGCAACCTCGGTCTGCCGCAGGATGTGATTATCAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAATCGACTACACTGGGGaaacggt                        <  2:26533/244‑5 (MQ=255)
                                                                                                                                                                                                                             gaagtGTACCTGGAAGGTGTTGACCCGCACCGTGGTTTGTTCATGTATTCCCTAATGAGCGCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGAACCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATGCTGCGTGTGTGGGTGGCATCAACCGACTACACCGGTGAAATTGCCGt                     <  2:76107/247‑1 (MQ=255)
                                                                                                                                                                                                                              cA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGttt                   <  1:400004/251‑1 (MQ=255)
                                                                                                                                                                                                                               aatgtaccgggAAGGTTCTGACCAGCCCCGTGGTTGGTTCATGTCTTTCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGACGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTTGGTGGCATCAACCGACTACTCCGGTGAAATGGCCGttt                   <  1:41558/242‑1 (MQ=255)
                                                                                                                                                                                                                                 tGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTACCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGCTGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTAGGCGCGGATATTCTGCGTCTGGGGGTGGCATCAACCGACGACACCGGTGAAATGGCCGTTTCt                 >  2:397495/1‑251 (MQ=255)
                                                                                                                                                                                                                                  gTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGt                                                                                                            <  1:22493/159‑1 (MQ=255)
                                                                                                                                                                                                                                  gTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGt                                                                                                            >  2:22493/1‑159 (MQ=255)
                                                                                                                                                                                                                                  gTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCt                 <  2:125602/250‑1 (MQ=255)
                                                                                                                                                                                                                                  gTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTg                <  1:306390/251‑1 (MQ=255)
                                                                                                                                                                                                                                         gAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAAATGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACAACGGTGAAATGGCCGTTTCTGACg             >  2:438118/1‑247 (MQ=255)
                                                                                                                                                                                                                                            ggTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCGTCAACCGACTACACCGGGGAAATGGCCGTTTCTGACGAGATCCt      >  2:305951/1‑251 (MQ=255)
                                                                                                                                                                                                                                                cTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGAAGAGATCCTGaa   >  2:456055/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 tGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGtt                    <  2:464925/232‑1 (MQ=255)
                                                                                                                                                                                                                                                 tGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGtt                    >  1:464925/1‑232 (MQ=255)
                                                                                                                                                                                                                                                  gACCAGCACCGTGGTTTGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGaaa  <  1:8143/249‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
AGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAA  >  NC_000913/23839‑24328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑