Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,899	T→C	D503D (GAT→GAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,899	0	T	C	95.2%	240.2 / 0.2	83	D503N (GAT→AAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base C (28/51);  total (31/52)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

TCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACA‑‑CATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCG  >  NC_000913/23658‑24127                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          gcAGCATGGATCAGAAAGGTCTGCGTGCGCAGGCACTGAAATAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTTGGGCGTACCGATGTCACTGTTCGTGCACAAAGACGCGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAg                                                                                                                                                                                                                                 <  2:317361/250‑1 (MQ=255)   aGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCa                                                                                                                                                                                                                                  >  1:268509/1‑248 (MQ=255)   aGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCa                                                                                                                                                                                                                                  <  2:268509/248‑1 (MQ=255)     cATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCgt                                                                                                                                                                                                                             >  1:310916/1‑251 (MQ=255)     cATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTAGCGAGTCGATTGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCgt                                                                                                                                                                                                                             <  2:372279/251‑1 (MQ=255)       gggATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCgtgg                                                                                                                                                                                                                           <  2:11229/250‑1 (MQ=255)             aGAAAGGTCTTCGTTCGGAGTCACTGAAAGAGCTCAAAGGCGTCCAGGGGAGCCCGGACTGGGGCCTGGCGCGTATCGAGTCGATGTGTGCTAACCGGCCGGACTGGTGTATCTCCCGTCAGCGCCCCTTGGGCGTACCTATGTTACTGTGCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGAt                                                                                                                                                                                                                     <  2:12686/251‑1 (MQ=255)             aGAAAGGTATGCGTGCGCAGGCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGAt                                                                                                                                                                                                                     <  2:160067/251‑1 (MQ=255)               aaagggtcgcgggcgcAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCCGGCGCGTCTCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGGTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGAtct                                                                                                                                                                                                                   <  2:121119/239‑1 (MQ=255)                   gTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGGGGGATCTCGAt                                                                                                                                                                                                               >  1:136332/1‑251 (MQ=255)                       gcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATg                                                                                                                                                                                                              <  1:160296/248‑1 (MQ=255)                         gtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGaaa                                                                                                                                                                                                         <  1:17086/251‑1 (MQ=255)                         gtgcgAAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCAAGGACTGGGGCCAGGCGCGTATCGAGTAGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGAACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCATCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATATCGATGCGaa                                                                                                                                                                                                          >  2:211550/1‑250 (MQ=255)                                      gAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGAGGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGc                                                                                                                                                                                             <  2:224620/250‑1 (MQ=255)                                        aaGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGTTGCGAAAGAGATCCTCGGcgac                                                                                                                                                                                          >  2:158470/1‑251 (MQ=255)                                          gagaTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcgacga                                                                                                                                                                                        <  2:135881/251‑1 (MQ=255)                                             aTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGc                                                                                                                                                                                     <  1:98778/251‑1 (MQ=255)                                                 aaGGCGGGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTCGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGac                                                                                                                                                                                 <  2:432094/251‑2 (MQ=255)                                                   ggCGGGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGGCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGacca                                                                                                                                                                               <  2:317464/251‑4 (MQ=255)                                                      gTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCAATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGt                                                                                                                                                                             >  1:476720/1‑250 (MQ=255)                                                          aGTGGATCCCGGACTGGGGCCAGGCGTGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGGACCGATGTCACTGGTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGTAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCGGACCAGTAtgtg                                                                                                                                                                        <  1:325697/251‑1 (MQ=255)                                                            tGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGa                                                                                                                                                                       >  1:136474/1‑250 (MQ=255)                                                             ggATCCCGGGCTGGTGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGTCTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGaaa                                                                                                                                                                     <  1:355536/251‑1 (MQ=255)                                                                 cccGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGc                                                                                                                                                                 <  1:345252/251‑1 (MQ=255)                                                                    ggACTGGGGCCAGGCGCGTATCGAGTCGATGGTCGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACATGGGGCGTACCGATGTCACTGTTCGTGCACCAAGACACGGACGAGCTGCATCCGCGTACCCTCGACCTGATGGCAGCAGTGGCAACACCAGTTGAAGTTACC‑GGCATCCAGGGGGGGGGGGATCGCGATACGACAGAGGTCCTCGGCACCGAAGCTGACCAGtgtgtgacggggccgg                                                                                                                                                              >  2:367436/1‑236 (MQ=255)                                                                      aCTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGat                                                                                                                                                            <  1:427020/251‑2 (MQ=255)                                                                       cTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCt                                                                                                                                                                                    <  2:108550/226‑1 (MQ=255)                                                                       cTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCt                                                                                                                                                                                    >  1:108550/1‑226 (MQ=255)                                                                       cTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGat                                                                                                                                                            >  2:408489/1‑249 (MQ=255)                                                                            gCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctc                                                                                                                                                      <  1:349993/251‑8 (MQ=255)                                                                               aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGAT‑GGc                                                                                                                                                                                                                                       >  1:250283/1‑167 (MQ=255)                                                                               aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                                                                                                                   >  1:396684/1‑241 (MQ=255)                                                                               aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                                                                                                                   <  1:57863/251‑11 (MQ=255)                                                                               aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCGGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGAT‑GGc                                                                                                                                                                                                                                       <  2:250283/167‑1 (MQ=255)                                                                                       aTCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCACCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtatggtt                                                                                                                                           <  2:262159/251‑19 (MQ=255)                                                                                       aTCGAGGCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtatggt                                                                                                                                            <  2:116818/250‑18 (MQ=255)                                                                                                aTGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCgg                                                                                                                               >  1:253088/1‑251 (MQ=255)                                                                                                  ggTTGCTAACCGTCCGGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTGGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCGCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑C‑TCGCGACGTATGGTTTGACTCCGGAt                                                                                                                             <  2:277396/251‑1 (MQ=255)                                                                                                       cTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAACGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGT‑TATCGGCATCCAGGCGTGGTGGGATCTCGCTGCGAAAGAGATCCTCGGCGACCAAGCTGACCAGTAGGTGAAAGTGCCGGATA‑‑CTCTC‑GACGGATGGTTTGACGCCGGATCtccc                                                                                                                        >  2:138466/1‑248 (MQ=255)                                                                                                         aaCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTAccc                                                                                                                       <  1:102422/250‑1 (MQ=255)                                                                                                         aaCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTAcc                                                                                                                        <  1:67659/249‑1 (MQ=255)                                                                                                          acattCCTGACTTGTGTAGTTCCCGTCAGCGAATAGGGGACGTATCGATGTCACTGATCGTGAACAAAGAAACGGAAGAGCTGCAACCGCGTCCCCACGAACTGATGGTAGAAGTGGCAAAACTCGTGGCAGTTAAC‑GGTACCCAGGCGTGGTGGGAGATCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTAAAAGTGCCGGATATTCAT‑‑‑GACGTATGGTTTGACTCCGTATCTACCCat                                                                                                                     <  2:2411/247‑2 (MQ=255)                                                                                                               ccTGGCTGGGGTATCGCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACtctt                                                                                                                 <  2:101891/250‑1 (MQ=255)                                                                                                               ccTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACtctt                                                                                                                 <  1:110932/250‑1 (MQ=255)                                                                                                                cTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGGGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACtcttct                                                                                                               >  2:2811/1‑251 (MQ=255)                                                                                                                 tGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTAAGGTTTGACTCCGGATCTACCCACTCTTCtg                                                                                                              >  2:432619/1‑251 (MQ=255)                                                                                                                    cTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACCGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGCAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACCCTTCtgttg                                                                                                           >  2:232415/1‑251 (MQ=255)                                                                                                                               cccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTCCCCACTCTTCTGTTGTTGACGTGCGt                                                                                                <  1:480933/251‑1 (MQ=255)                                                                                                                                ccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTc                                                                                               >  1:471822/1‑251 (MQ=255)                                                                                                                                                 gcgtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCAc                                                                              >  1:384567/3‑251 (MQ=255)                                                                                                                                                   gtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTTACCAGTATGTGAAATTGCCGGATA‑‑CTTTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGc                                                                            <  1:317329/251‑1 (MQ=255)                                                                                                                                                     aCCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACA‑‑CATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGc                                                                            >  1:115482/1‑249 (MQ=255)                                                                                                                                                        gATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGTTCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGACTTTGCCGGTCACGCAGCgg                                                                       >  2:132675/1‑251 (MQ=255)                                                                                                                                                         aTGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGa                                                                      <  2:476720/251‑1 (MQ=255)                                                                                                                                                             cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑t                                                                  <  1:31894/250‑1 (MQ=255)                                                                                                                                                             cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑Tg                                                                 <  1:158470/251‑1 (MQ=255)                                                                                                                                                             cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑Tg                                                                 <  2:47776/251‑1 (MQ=255)                                                                                                                                                                  ttCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTCCC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTACCGCAGCggcctagtatcc                                                             >  2:437855/1‑241 (MQ=255)                                                                                                                                                                        cacaAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGAAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGCGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGAATCCGGATCTACCCACTCTTCTGTTGTTGCCGTGGGTCCGGAATTTGCCGGTCACGAAACGGGAA‑TGGATCTGGAAgg                                                      >  2:234815/1‑251 (MQ=255)                                                                                                                                                                         acaAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGt                                                     <  1:193222/251‑1 (MQ=255)                                                                                                                                                                               acacGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGAc                                               <  2:215461/251‑1 (MQ=255)                                                                                                                                                                               acacGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGAc                                               <  1:247324/251‑1 (MQ=255)                                                                                                                                                                                cacGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGAcc                                              <  1:197507/251‑1 (MQ=255)                                                                                                                                                                                   ggAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACC‑TGTATCTGGAAGGTTCTGCCCagc                                           >  2:359122/1‑249 (MQ=255)                                                                                                                                                                                      agagCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCAcc                                        <  1:299602/251‑1 (MQ=255)                                                                                                                                                                                       gagCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCGAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACcg                                       <  2:464702/251‑1 (MQ=255)                                                                                                                                                                                       gagCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACcg                                       <  1:233390/251‑1 (MQ=255)                                                                                                                                                                                         gatGCATCCGCGTACCCTCGAACTGTAGGAAGAAGTGGCAAAACGCGTTTAAGTTAAC‑TGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACcgtg                                     <  1:287138/249‑3 (MQ=255)                                                                                                                                                                                            gCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GAAGTATGGTTTGGCTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTATGGCCAGCACcgtggtt                                  >  2:86077/1‑246 (MQ=255)                                                                                                                                                                                             cATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTGGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACcgtggttg                                 <  2:384567/251‑7 (MQ=255)                                                                                                                                                                                              aTCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGAGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTACGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGCCA‑TGTATCTGGAAGGTTCTGACCAGCACcgtggttgg                                >  1:224604/1‑244 (MQ=255)                                                                                                                                                                                                ccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGtggttggt                               <  1:205248/250‑1 (MQ=255)                                                                                                                                                                                                       cccTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCt                       <  1:363694/251‑1 (MQ=255)                                                                                                                                                                                                           cgAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTccc                   >  2:333697/2‑251 (MQ=255)                                                                                                                                                                                                            gAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCt                  <  2:150542/251‑1 (MQ=255)                                                                                                                                                                                                             aaCTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCt                  >  2:456419/1‑250 (MQ=255)                                                                                                                                                                                                              acggATGGAAGAAGGGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGCAGCTGACCAGTATGTGGAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGGTCTACCCACGCTTCTGTTGTTGTCGCGCGTCCGGCATTTGCCGGGAACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCCTCCCTat                >  2:161011/4‑250 (MQ=255)                                                                                                                                                                                                                 gATGGAAGAAGTGGCAAAACGCGTTTGAGTTAAC‑GGCCCCCAGGCGTGGTTGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCCCCGTTGTTGGTTCATGTCTTCCCTAAt               <  2:297164/249‑1 (MQ=255)                                                                                                                                                                                                                  aTGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGACTTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAt            >  2:74095/1‑251 (MQ=255)                                                                                                                                                                                                                     gaagaaGTGGTACAACGCGTTGAAGTCAAC‑GGCATCAAGGCGTGATGAGATCTCGATGCGCAAGAGATCCTCTGCGATGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCGGTTGTTGACTTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCATCACCGTGGTTGGTTCATGTCGTCCCTaagtatctc         <  2:202036/251‑8 (MQ=255)                                                                                                                                                                                                                       agaagTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCa       <  2:77394/251‑1 (MQ=255)                                                                                                                                                                                                                         aagTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCAcc     <  1:198477/251‑1 (MQ=255)                                                                                                                                                                                                                            tGGCAAAACGCGTTGAAGTTAAC‑GGCATCCATGAGTGGTGGGATCTCGATGCAAAAGAGATCCTCGGCGACGAATCTGACAAGTATGTGAACGTGACGGATA‑‑CTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGG‑CACTGTATCTGGACGGTTCTGCACAGCCCCGTGGTTGGTTCATGCCTTCCCTAATAATCTCCACcgcg  >  2:268165/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          TCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACA‑‑CATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACA‑TGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCG  >  NC_000913/23658‑24127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑