Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,270	G→A	G294S (GGC→AGC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,270	0	G	A	96.4%	160.9 / NA	56	G294S (GGC→AGC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (33/20);  minor base C (1/1);  total (35/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAC  >  NC_000913/23023‑23515                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       cGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCa                                                                                                                                                                                                                                                     <  1:366276/251‑1 (MQ=255)                       cAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTg                                                                                                                                                                                                                                <  2:123181/250‑1 (MQ=255)                       cAGGAAGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTg                                                                                                                                                                                                                                <  2:269122/250‑1 (MQ=255)                          gATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTAGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAggt                                                                                                                                                                                                                            >  1:160067/1‑249 (MQ=255)                             gCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGAgttgct                                                                                                                                                                                                                         >  1:64731/1‑251 (MQ=255)                              cACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGAGttgctg                                                                                                                                                                                                                        >  1:411951/1‑251 (MQ=255)                                    aaGCAAAATTTGCAGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGAGCTGGACCACTACGCCGTGGTCTCTGTCTGCGAACCGCGCAATCTCTAGTGCACCTGATTTCGATTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGAACTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCttt                                                                                                                                                                                                                  <  2:298042/251‑1 (MQ=255)                                      gCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCACGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTAc                                                                                                                                                                                                                >  2:461066/1‑251 (MQ=255)                                         aaaTTTGCAGGAATCAACGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCGCAGCACGGTAAAAGGTGCGGAGCTTGATTTGCTGCGCTTTACCCa                                                                                                                                                                                                             <  2:209806/251‑1 (MQ=255)                                              tGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCg                                                                                                                                                                                                         <  1:197707/250‑1 (MQ=255)                                              tGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGATGCGGAGCTTGAATTGCTGCGCTTTACCCATCCGt                                                                                                                                                                                                        >  1:234697/1‑251 (MQ=255)                                                  gTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGTGAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTAt                                                                                                                                                                                                    >  2:116874/1‑251 (MQ=255)                                                  gTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGAATCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAAGCCGTGATTCTGGCGAAAGATCTGGTTGAACGCGTAATGCAGCGTATCGGCGTGACCGATTCCACCATTCTCAGCGCGGTCAAAGGTGCGGAGATTTAGTTGCTGCGCTTTACCCCTCCGttttt                                                                                                                                                                                                    >  2:288516/1‑249 (MQ=255)                                                   tAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATg                                                                                                                                                                                                   >  1:148243/1‑251 (MQ=255)                                                     aGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAT‑ACACCATTCTCAGCACGGTAAAAGGGGCGGGGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGc                                                                                                                                                                                                >  1:227496/1‑251 (MQ=255)                                                        aaCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGGGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCATATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGTAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCtt                                                                                                                                                                                              <  2:401874/251‑1 (MQ=255)                                                          cGTTAACGTCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCg                                                                                                                                                                                            <  1:291916/251‑1 (MQ=255)                                                           gTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGGAATGCAGCGTATCGGCGTGGCCGATTACACCCATCTCAGCACGGGAAAAGGGGCGGAGCGTGGATTGCTGCGCTTTACCCATCCGTTTATGGGCTTCgc                                                                                                                                                                                           >  1:238813/1‑250 (MQ=255)                                                                  gCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCg                                                                                                                                                                                    >  2:8719/1‑251 (MQ=255)                                                                           cGCTGGTGATCTGGACCCCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAT‑ACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCg                                                                                                                                                                          <  2:227496/251‑1 (MQ=255)                                                                           cGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                                                                                                                                           <  1:254483/251‑1 (MQ=255)                                                                                     cTGGACCACTACGCCGTGGACTCTGCCTGCGAACCCCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATcccg                                                                                                                                                                 >  2:125384/1‑248 (MQ=255)                                                                                      tGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑Gt                                                                                                                                                               >  1:48425/1‑251 (MQ=255)                                                                                             ctacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGGGCTTGGGTTTCTGCGCTTTTCCCCTCCGTTTATGGGCTTCGGCGTTCCGGCCATCCTCTGCGATCAC‑CTTACCCTg                                                                                                                                                        >  2:406503/3‑251 (MQ=255)                                                                                                  ccGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGAT‑ACAGTTACCCTGGATGc                                                                                                                                                   >  2:385681/1‑251 (MQ=255)                                                                                                     tGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTAACCGATTACACCCTTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTTACCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTAGGCGATCCC‑GTTACCCTGGATGCCgg                                                                                                                                                >  2:219809/1‑251 (MQ=255)                                                                                                                            aaTCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCCTTACCCATCCGTTTATGGGCTTCGGCGTTCCGGCAACCCTCGTACATCAC‑GTTACCCTGGAGGCCGGGACCGGGGACGTTTACACCccgc                                                                                                                         >  2:330352/1‑251 (MQ=255)                                                                                                                                  tATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTTACCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCCC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCCCCCCCCGCCTGGcc                                                                                                                   >  2:45356/1‑251 (MQ=255)                                                                                                                                       cACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGTAGACAGATCTGGTTGCAAGCGCAATGCAACGTATCGGCGTGACAGATTACACAATTCTCCGCACGGTAAAAGGTGCGGAGCTTCAGTTGCTGCGCTTTACCCATCCGTTATTGGGCTTCGCCGTTCCGGCAATACTCCGAGCTCAA‑GTTCCCATGGATACCCGTCACGGTCCCGTTCACACCCAGCCTGGCCAccgc                                                                                                              >  1:209820/1‑248 (MQ=255)                                                                                                                                           tgATTTCGACTATGCGCTGGTGCAGATCGACGGTCACGCCGTAATTTTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGATTGAGTTGATGCGCTTTAACCATCCGGTTATGGGGTTCGCAGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCAGGCTGCCTGTACCGGGGCAGTTCCCACCGCGCCTGGCCACAGccc                                                                                                            >  2:17086/2‑249 (MQ=255)                                                                                                                                             aTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgac                                                                                                        <  2:64731/251‑1 (MQ=255)                                                                                                                                                 cGACTATGCGCTGGTGCAGATCAACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACt                                                                                                    <  1:345911/251‑1 (MQ=255)                                                                                                                                                   aCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGCTCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGAGCGGAGCTTGAGTTGCTGCGCTTTACCCATCAGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGCACAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTAGCCACGGCCCGGACGACTAt                                                                                                  >  1:186899/1‑251 (MQ=255)                                                                                                                                                        gctcTGGTGCAGATCGACTGTCAGGCCGTGATTCTGGCGAAAGATCTGTTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACTCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGGTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGAt                                                                                             <  1:455223/248‑1 (MQ=255)                                                                                                                                                        gcgcTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGGGCCGTTCACACCGCGCCTGGCCCCGGCCCGGGCGACTAtgtgtt                                                                                             >  1:377412/1‑249 (MQ=255)                                                                                                                                                         cgcTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCCATCCTCGGCGAACAC‑GTTACCCTGGAAGCCGGTACCGGGGACGTTCACACCGCCCCCGGCCACGGCCCggctgcctaatggatc                                                                                            >  2:98778/1‑237 (MQ=255)                                                                                                                                                                   gATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAAt                                                                                  >  1:176591/1‑251 (MQ=255)                                                                                                                                                                    aTCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTAACGGTGCCGTTCCCACCGCGCCTGGCCCCGGCCCGGACGACTATGTGGTCGGTCAGa                                                                                     >  1:266074/1‑247 (MQ=255)                                                                                                                                                                    aTCGACGGTCAGGCCGGGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGa                                                                                     <  2:266074/247‑1 (MQ=255)                                                                                                                                                                               ggCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGaaa                                                                      <  1:8719/251‑1 (MQ=255)                                                                                                                                                                                                 aGATCTGGGTGAAAGCGGAATGCAGCGTATCGGAGTGACCGATTACGCCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGATTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGATCCGGCAATCCTCGGCGATCAC‑GTTACCCTTGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGcc                                                    <  2:186319/251‑1 (MQ=255)                                                                                                                                                                                                         ttGAAATCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGc                                            >  1:233948/1‑251 (MQ=255)                                                                                                                                                                                                          tGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACCATGTGATCGGGCAGAAATACGGCCTGGAAACCGCCAACCCGGTAGGGCcggccggcc                                           >  1:262159/1‑250 (MQ=255)                                                                                                                                                                                                           gAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCAc                                          >  1:464358/1‑251 (MQ=255)                                                                                                                                                                                                             aaGCGTAATGCAGCGTATCGGCGTTACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACtt                                        <  2:48425/251‑1 (MQ=255)                                                                                                                                                                                                             aaGCGTAATGCAGCGTATCGGCGTGACCGATTACACCCTTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGGTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGTTCGGTCAGAAATACGGCCTGGAAACCGCAACCCCCGGTGGGCCGGACGGCCCtt                                        >  2:120076/1‑251 (MQ=255)                                                                                                                                                                                                              aGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTa                                       <  1:461066/251‑1 (MQ=255)                                                                                                                                                                                                                        aGCGTACCGGCGTGACCGATTACACCATTATAAACCCGGTAAAAGGGGCGCAGCTTGAGTTGATGCGCTTTACCCATCCGTTTCTGGGCTTCGACGTTCCGGCAATCCTCGGCGACCAC‑GTTACCCTGGCTGCCGGTACCGGATCCGTTCACACAGCGCCTTGCCACGGCCCGGGCGACTAAGTGAACGGTCCGAAATACGGCCCGGACACCGCCAAACCCGTTGGCCCGACCCTCACTGATCTGCCGGGc                             >  2:197507/1‑251 (MQ=255)                                                                                                                                                                                                                              tCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGTGCGGAGCTTGAGTGGCGGCGCTTTACCCATCCGGTTATGGGTTTCGACGTTCCGGCAACCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTCCCGGTGCCGTTCACTCCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTa                        <  1:288516/250‑1 (MQ=255)                                                                                                                                                                                                                                  cGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTAAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCg                    >  2:315266/1‑250 (MQ=255)                                                                                                                                                                                                                                   gTGACCGATTACACCATTCTCAGCAAGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGCCGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGCTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTCTGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCCCTTCTCTTCCGGGCACTTATCCGAc                  >  1:317361/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGCTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATAACGCCTGGAAACCGCTAACCCGTTTGGCCCGTGCGGCACTTATCTGCCGGGCACTTATCCGAAGCTgg             >  2:363694/1‑251 (MQ=255)                                                                                                                                                                                                                                             acacCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGc        >  2:157435/1‑251 (MQ=255)                                                                                                                                                                                                                                             acacCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGc        <  1:154179/251‑1 (MQ=255)                                                                                                                                                                                                                                              cacCATTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGGAGCCGATAACCCGGTTGGCCCGGACGGCGCTTATCTGCCGGGCACTTATCCGACGCGTGATGGCg       >  2:198477/1‑251 (MQ=255)                                                                                                                                                                                                                                                    tctcAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTTCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAc  <  2:5520/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       CGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAC  >  NC_000913/23023‑23515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑