Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,161	A→T	P257P (CCA→CCT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,161	0	A	T	100.0%	188.6 / NA	58	P257P (CCA→CCT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (38/20);  total (38/20)

GGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGC  >  NC_000913/22916‑23407                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        ggTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAttt                                                                                                                                                                                                                                                    >  1:117997/1‑251 (MQ=255) ggTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAttt                                                                                                                                                                                                                                                    >  2:98103/1‑251 (MQ=255)                 gcgcGAAGACGGTACACTGGTGCGTTGACTGCCGTCCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAACACAAAATTTGCCGTAAGCAACGTTAAAGGCCCAGTCTCGCTGGTGATCTGTCCCCCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCAATTTCCCCTGCTTTCGACTATGCGCTGGTg                                                                                                                                                                                                                                    >  2:32458/1‑251 (MQ=255)                    cgAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGCCTATGCGCTGGTGc                                                                                                                                                                                                                                   >  1:89218/1‑249 (MQ=255)                        gccggtacACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTAGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGc                                                                                                                                                                                                                                   >  1:233736/8‑245 (MQ=255)                           ggtacACTGGTGCTTTGACTGCCGTTCTGCGCTTGCGGAAGCGGGCGTTGCGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGCTCAGGCTTCACTGAAAGCAAAATTTACCGTAAGCAACGTTAACGGCCCCCTCTCGCTGTTGATCTATACCCCTACTCCGTTGACTCTGCCTCCCAACCGCTCACTCTCTTTTTCCCCTTTTTTCGTCTATGCGCTGGTGCAggtccacc                                                                                                                                                                                                                          >  2:35812/5‑244 (MQ=255)                                              gtcggTTCTGCGCTTGCGGAAGCGGAAGTTGAGTATTACGACAAAACCTCTCCGTCCATCGACGTGGAGTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTCGCCGTAAGCAACGTGAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCTCTACTGCACCTGATTTCGTCTAGGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTgga                                                                                                                                                                                                       <  2:166884/247‑2 (MQ=255)                                                            ggCGTAAGCGGAAGTTGTGTATTACGACAAAACTTCTCCGTCCAGCGACGTTGCTTTTCAGGCGGTCGATCATGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCTTGTACTCTGCCTGCGAACCGCGACATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCATATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGtt                                                                                                                                                                                          <  2:407693/250‑1 (MQ=255)                                                               ggaagcgtaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCCCGCTGGTGATCTGGACCACTACGCCGTGGAGTCTGACTGCGAAACGGCCAATCTCTATTGCAACTGATTTCGACTACGCGCTGGTGCAGGTCGACGGTCAGGCCGTGATTTTGGCGAAAgctagagttgac                                                                                                                                                                                       >  2:153141/1‑239 (MQ=255)                                                                   gcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGt                                                                                                                                                                                  >  1:269122/1‑251 (MQ=255)                                                                         gTTGTGTATTACGACAAAACTTCCCCGTCCAACGACGTTGCTTTTCAGGCGGTAGATCAGGATGCACTGAAAGCAAAATTCGCCGTAAGCAACGTTAACGGACCAATCTCGCAGGGGATCTGGACCACTACGCCGTGGACTCTGCCTGCGACCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTTGTCGAAAGCGTAATGCa                                                                                                                                                                            <  2:106332/251‑1 (MQ=255)                                                                          ttGAGTATTACGCCAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCCGGCCGTGATTCTTGCGAAAAATCTGTTTGAAAGCGTAAtccgg                                                                                                                                                                           >  2:355536/1‑247 (MQ=255)                                                                           tGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGTCTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGc                                                                                                                                                                          >  2:206350/1‑251 (MQ=255)                                                                                   ccGACAAAACTTCGCCGTCCAGCGTCGTTGCTTTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTTACGGCCCAATCGCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGc                                                                                                                                                                  <  2:221645/250‑1 (MQ=255)                                                                                   aCGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCACCGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCgg                                                                                                                                                                   <  2:273930/250‑1 (MQ=255)                                                                                       cAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTgg                                                                                                                                                              >  2:151927/1‑250 (MQ=255)                                                                                        aaaaCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc                                                                                                                                                             >  1:189799/1‑251 (MQ=255)                                                                                        aaaaCTGCACCGTCCAGCGACGTTGCTTTTCAGGCGGTCTATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc                                                                                                                                                             <  2:23536/251‑1 (MQ=255)                                                                                         aaaCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGGTCTGGTTGAAAGCGTAATGCAGCGTGTCGGCGTGAcc                                                                                                                                                            >  2:127087/1‑251 (MQ=255)                                                                                         aaaCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAcc                                                                                                                                                            <  1:6549/251‑1 (MQ=255)                                                                                            cTTCTCCGTACATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCACAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACAGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGCTCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCggt                                                                                                                                                         >  2:436398/1‑249 (MQ=255)                                                                                                tcCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTac                                                                                                                                                      >  1:260096/1‑250 (MQ=255)                                                                                                  cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGCTGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGAAGCGTATCGGCGTGACCGATTACAcc                                                                                                                                                   >  2:150008/1‑251 (MQ=255)                                                                                                          gACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcag                                                                                                                                           <  2:346915/251‑3 (MQ=255)                                                                                                            cGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcagcc                                                                                                                                         >  1:5520/1‑247 (MQ=255)                                                                                                            cGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCa                                                                                                                                         <  1:366276/251‑1 (MQ=255)                                                                                                             gTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCCGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcaaccc                                                                                                                                        >  2:266037/1‑246 (MQ=255)                                                                                                                        ggcggTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTg                                                                                                                                                                                                               <  1:475583/165‑1 (MQ=255)                                                                                                                                  cAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTg                                                                                                                    <  2:123181/250‑1 (MQ=255)                                                                                                                                  cAGGAAGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTg                                                                                                                    <  2:269122/250‑1 (MQ=255)                                                                                                                                     gATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctca                                                                                                                                            <  2:76022/223‑2 (MQ=255)                                                                                                                                     gATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctca                                                                                                                                            >  1:76022/1‑222 (MQ=255)                                                                                                                                     gATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTAGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAggt                                                                                                                >  1:160067/1‑249 (MQ=255)                                                                                                                                        gCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGAgttgct                                                                                                             >  1:64731/1‑251 (MQ=255)                                                                                                                                         cACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGAGttgctg                                                                                                            >  1:411951/1‑251 (MQ=255)                                                                                                                                               aaGCAAAATTTGCAGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGAGCTGGACCACTACGCCGTGGTCTCTGTCTGCGAACCGCGCAATCTCTAGTGCACCTGATTTCGATTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGAACTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCttt                                                                                                      <  2:298042/251‑1 (MQ=255)                                                                                                                                                 gCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCACGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTAc                                                                                                    >  2:461066/1‑251 (MQ=255)                                                                                                                                                    aaaTTTGCAGGAATCAACGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCGCAGCACGGTAAAAGGTGCGGAGCTTGATTTGCTGCGCTTTACCCa                                                                                                 <  2:209806/251‑1 (MQ=255)                                                                                                                                                         tGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCg                                                                                             <  1:197707/250‑1 (MQ=255)                                                                                                                                                         tGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGATGCGGAGCTTGAATTGCTGCGCTTTACCCATCCGt                                                                                            >  1:234697/1‑251 (MQ=255)                                                                                                                                                             gTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGTGAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTAt                                                                                        >  2:116874/1‑251 (MQ=255)                                                                                                                                                             gTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGAATCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAAGCCGTGATTCTGGCGAAAGATCTGGTTGAACGCGTAATGCAGCGTATCGGCGTGACCGATTCCACCATTCTCAGCGCGGTCAAAGGTGCGGAGATTTAGTTGCTGCGCTTTACCCCTCCGttttt                                                                                        >  2:288516/1‑249 (MQ=255)                                                                                                                                                              tAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATg                                                                                       >  1:148243/1‑251 (MQ=255)                                                                                                                                                                aGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAT‑ACACCATTCTCAGCACGGTAAAAGGGGCGGGGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGc                                                                                    >  1:227496/1‑251 (MQ=255)                                                                                                                                                                   aaCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGGGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCATATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGTAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCtt                                                                                  <  2:401874/251‑1 (MQ=255)                                                                                                                                                                     cGTTAACGTCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCg                                                                                <  1:291916/251‑1 (MQ=255)                                                                                                                                                                      gTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGGAATGCAGCGTATCGGCGTGGCCGATTACACCCATCTCAGCACGGGAAAAGGGGCGGAGCGTGGATTGCTGCGCTTTACCCATCCGTTTATGGGCTTCgc                                                                               >  1:238813/1‑250 (MQ=255)                                                                                                                                                                             gCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCg                                                                        >  2:8719/1‑251 (MQ=255)                                                                                                                                                                                      cGCTGGTGATCTGGACCCCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAT‑ACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCg                                                              <  2:227496/251‑1 (MQ=255)                                                                                                                                                                                      cGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                               <  1:254483/251‑1 (MQ=255)                                                                                                                                                                                                cTGGACCACTACGCCGTGGACTCTGCCTGCGAACCCCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATcccg                                                     >  2:125384/1‑248 (MQ=255)                                                                                                                                                                                                 tGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑Gt                                                   >  1:48425/1‑251 (MQ=255)                                                                                                                                                                                                        ctacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGGGCTTGGGTTTCTGCGCTTTTCCCCTCCGTTTATGGGCTTCGGCGTTCCGGCCATCCTCTGCGATCAC‑CTTACCCTg                                            >  2:406503/3‑251 (MQ=255)                                                                                                                                                                                                             ccGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGAT‑ACAGTTACCCTGGATGc                                       >  2:385681/1‑251 (MQ=255)                                                                                                                                                                                                                tGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTAACCGATTACACCCTTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTTACCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTAGGCGATCCC‑GTTACCCTGGATGCCgg                                    >  2:219809/1‑251 (MQ=255)                                                                                                                                                                                                                                       aaTCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCCTTACCCATCCGTTTATGGGCTTCGGCGTTCCGGCAACCCTCGTACATCAC‑GTTACCCTGGAGGCCGGGACCGGGGACGTTTACACCccgc             >  2:330352/1‑251 (MQ=255)                                                                                                                                                                                                                                             tATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTTACCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCCC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCCCCCCCCGCCTGGcc       >  2:45356/1‑251 (MQ=255)                                                                                                                                                                                                                                                  cACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGTAGACAGATCTGGTTGCAAGCGCAATGCAACGTATCGGCGTGACAGATTACACAATTCTCCGCACGGTAAAAGGTGCGGAGCTTCAGTTGCTGCGCTTTACCCATCCGTTATTGGGCTTCGCCGTTCCGGCAATACTCCGAGCTCAA‑GTTCCCATGGATACCCGTCACGGTCCCGTTCACACCCAGCCTGGCCAccgc  >  1:209820/1‑248 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        GGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGC  >  NC_000913/22916‑23407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑