Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,137	C→G	A249A (GCC→GCG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,137	0	C	G	98.4%	193.0 / NA	61	A249A (GCC→GCG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (37/23);  total (38/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGG  >  NC_000913/22896‑23373                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              tGGGCAAAATCATCGGCAACGGTCTCCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCgcgc                                                                                                                                                                                                                                       >  1:123181/1‑250 (MQ=255)        aaTCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTa                                                                                                                                                                                                                               <  1:239649/251‑1 (MQ=255)         atcatcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTAt                                                                                                                                                                                                                              <  1:227793/251‑1 (MQ=255)            atcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGc                                                                                                                                                                                                                           >  1:143641/1‑251 (MQ=255)              cGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAc                                                                                                                                                                                                                         >  1:296228/1‑251 (MQ=255)                gCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAcct                                                                                                                                                                                                                       <  1:394655/251‑2 (MQ=255)                     ggTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAttt                                                                                                                                                                                                                  >  1:117997/1‑251 (MQ=255)                     ggTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAttt                                                                                                                                                                                                                  >  2:98103/1‑251 (MQ=255)                                     gcgcGAAGACGGTACACTGGTGCGTTGACTGCCGTCCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAACACAAAATTTGCCGTAAGCAACGTTAAAGGCCCAGTCTCGCTGGTGATCTGTCCCCCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCAATTTCCCCTGCTTTCGACTATGCGCTGGTg                                                                                                                                                                                                  >  2:32458/1‑251 (MQ=255)                                        cgAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGCCTATGCGCTGGTGc                                                                                                                                                                                                 >  1:89218/1‑249 (MQ=255)                                            gccggtacACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTAGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGc                                                                                                                                                                                                 >  1:233736/8‑245 (MQ=255)                                               ggtacACTGGTGCTTTGACTGCCGTTCTGCGCTTGCGGAAGCGGGCGTTGCGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGCTCAGGCTTCACTGAAAGCAAAATTTACCGTAAGCAACGTTAACGGCCCCCTCTCGCTGTTGATCTATACCCCTACTCCGTTGACTCTGCCTCCCAACCGCTCACTCTCTTTTTCCCCTTTTTTCGTCTATGCGCTGGTGCAggtccacc                                                                                                                                                                                        >  2:35812/5‑244 (MQ=255)                                                                  gtcggTTCTGCGCTTGCGGAAGCGGAAGTTGAGTATTACGACAAAACCTCTCCGTCCATCGACGTGGAGTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTCGCCGTAAGCAACGTGAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCTCTACTGCACCTGATTTCGTCTAGGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTgga                                                                                                                                                                     <  2:166884/247‑2 (MQ=255)                                                                                ggCGTAAGCGGAAGTTGTGTATTACGACAAAACTTCTCCGTCCAGCGACGTTGCTTTTCAGGCGGTCGATCATGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCTTGTACTCTGCCTGCGAACCGCGACATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCATATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGtt                                                                                                                                                        <  2:407693/250‑1 (MQ=255)                                                                                   ggaagcgtaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCCCGCTGGTGATCTGGACCACTACGCCGTGGAGTCTGACTGCGAAACGGCCAATCTCTATTGCAACTGATTTCGACTACGCGCTGGTGCAGGTCGACGGTCAGGCCGTGATTTTGGCGAAAgctagagttgac                                                                                                                                                     >  2:153141/1‑239 (MQ=255)                                                                                       gcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGt                                                                                                                                                >  1:269122/1‑251 (MQ=255)                                                                                             gTTGTGTATTACGACAAAACTTCCCCGTCCAACGACGTTGCTTTTCAGGCGGTAGATCAGGATGCACTGAAAGCAAAATTCGCCGTAAGCAACGTTAACGGACCAATCTCGCAGGGGATCTGGACCACTACGCCGTGGACTCTGCCTGCGACCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTTGTCGAAAGCGTAATGCa                                                                                                                                          <  2:106332/251‑1 (MQ=255)                                                                                              ttGAGTATTACGCCAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCCGGCCGTGATTCTTGCGAAAAATCTGTTTGAAAGCGTAAtccgg                                                                                                                                         >  2:355536/1‑247 (MQ=255)                                                                                               tGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGTCTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGc                                                                                                                                        >  2:206350/1‑251 (MQ=255)                                                                                                       ccGACAAAACTTCGCCGTCCAGCGTCGTTGCTTTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTTACGGCCCAATCGCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGc                                                                                                                                <  2:221645/250‑1 (MQ=255)                                                                                                       aCGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCACCGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCgg                                                                                                                                 <  2:273930/250‑1 (MQ=255)                                                                                                           cAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTgg                                                                                                                            >  2:151927/1‑250 (MQ=255)                                                                                                            aaaaCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc                                                                                                                           >  1:189799/1‑251 (MQ=255)                                                                                                            aaaaCTGCACCGTCCAGCGACGTTGCTTTTCAGGCGGTCTATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc                                                                                                                           <  2:23536/251‑1 (MQ=255)                                                                                                             aaaCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGGTCTGGTTGAAAGCGTAATGCAGCGTGTCGGCGTGAcc                                                                                                                          >  2:127087/1‑251 (MQ=255)                                                                                                             aaaCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAcc                                                                                                                          <  1:6549/251‑1 (MQ=255)                                                                                                                cTTCTCCGTACATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCACAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACAGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGCTCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCggt                                                                                                                       >  2:436398/1‑249 (MQ=255)                                                                                                                    tcCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTac                                                                                                                    >  1:260096/1‑250 (MQ=255)                                                                                                                      cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGCTGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGAAGCGTATCGGCGTGACCGATTACAcc                                                                                                                 >  2:150008/1‑251 (MQ=255)                                                                                                                              gACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcag                                                                                                         <  2:346915/251‑3 (MQ=255)                                                                                                                                cGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcagcc                                                                                                       >  1:5520/1‑247 (MQ=255)                                                                                                                                cGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCa                                                                                                       <  1:366276/251‑1 (MQ=255)                                                                                                                                 gTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCCGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcaaccc                                                                                                      >  2:266037/1‑246 (MQ=255)                                                                                                                                            ggcggTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTg                                                                                                                                                                             <  1:475583/165‑1 (MQ=255)                                                                                                                                                      cAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTg                                                                                  <  2:123181/250‑1 (MQ=255)                                                                                                                                                      cAGGAAGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTg                                                                                  <  2:269122/250‑1 (MQ=255)                                                                                                                                                         gATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctca                                                                                                          <  2:76022/223‑2 (MQ=255)                                                                                                                                                         gATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctca                                                                                                          >  1:76022/1‑222 (MQ=255)                                                                                                                                                         gATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTAGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAggt                                                                              >  1:160067/1‑249 (MQ=255)                                                                                                                                                            gCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGAgttgct                                                                           >  1:64731/1‑251 (MQ=255)                                                                                                                                                             cACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGAGttgctg                                                                          >  1:411951/1‑251 (MQ=255)                                                                                                                                                                   aaGCAAAATTTGCAGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGAGCTGGACCACTACGCCGTGGTCTCTGTCTGCGAACCGCGCAATCTCTAGTGCACCTGATTTCGATTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGAACTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCttt                                                                    <  2:298042/251‑1 (MQ=255)                                                                                                                                                                     gCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCACGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTAc                                                                  >  2:461066/1‑251 (MQ=255)                                                                                                                                                                        aaaTTTGCAGGAATCAACGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCGCAGCACGGTAAAAGGTGCGGAGCTTGATTTGCTGCGCTTTACCCa                                                               <  2:209806/251‑1 (MQ=255)                                                                                                                                                                             tGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCg                                                           <  1:197707/250‑1 (MQ=255)                                                                                                                                                                             tGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGATGCGGAGCTTGAATTGCTGCGCTTTACCCATCCGt                                                          >  1:234697/1‑251 (MQ=255)                                                                                                                                                                                 gTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGTGAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTAt                                                      >  2:116874/1‑251 (MQ=255)                                                                                                                                                                                 gTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGAATCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAAGCCGTGATTCTGGCGAAAGATCTGGTTGAACGCGTAATGCAGCGTATCGGCGTGACCGATTCCACCATTCTCAGCGCGGTCAAAGGTGCGGAGATTTAGTTGCTGCGCTTTACCCCTCCGttttt                                                      >  2:288516/1‑249 (MQ=255)                                                                                                                                                                                  tAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATg                                                     >  1:148243/1‑251 (MQ=255)                                                                                                                                                                                    aGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAT‑ACACCATTCTCAGCACGGTAAAAGGGGCGGGGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGc                                                  >  1:227496/1‑251 (MQ=255)                                                                                                                                                                                       aaCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGGGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCATATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGTAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCtt                                                <  2:401874/251‑1 (MQ=255)                                                                                                                                                                                         cGTTAACGTCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCg                                              <  1:291916/251‑1 (MQ=255)                                                                                                                                                                                          gTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGGAATGCAGCGTATCGGCGTGGCCGATTACACCCATCTCAGCACGGGAAAAGGGGCGGAGCGTGGATTGCTGCGCTTTACCCATCCGTTTATGGGCTTCgc                                             >  1:238813/1‑250 (MQ=255)                                                                                                                                                                                                 gCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCg                                      >  2:8719/1‑251 (MQ=255)                                                                                                                                                                                                          cGCTGGTGATCTGGACCCCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAT‑ACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCg                            <  2:227496/251‑1 (MQ=255)                                                                                                                                                                                                          cGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                             <  1:254483/251‑1 (MQ=255)                                                                                                                                                                                                                    cTGGACCACTACGCCGTGGACTCTGCCTGCGAACCCCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATcccg                   >  2:125384/1‑248 (MQ=255)                                                                                                                                                                                                                     tGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑Gt                 >  1:48425/1‑251 (MQ=255)                                                                                                                                                                                                                            ctacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGGGCTTGGGTTTCTGCGCTTTTCCCCTCCGTTTATGGGCTTCGGCGTTCCGGCCATCCTCTGCGATCAC‑CTTACCCTg          >  2:406503/3‑251 (MQ=255)                                                                                                                                                                                                                                 ccGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGAT‑ACAGTTACCCTGGATGc     >  2:385681/1‑251 (MQ=255)                                                                                                                                                                                                                                    tGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTAACCGATTACACCCTTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTTACCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTAGGCGATCCC‑GTTACCCTGGATGCCgg  >  2:219809/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              TGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCAC‑GTTACCCTGGATGCCGG  >  NC_000913/22896‑23373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑