Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	22,945	T→A	V185V (GTT→GTA)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	22,945	0	T	A	98.2%	166.8 / ‑6.7	55	V185V (GTT→GTA)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (32/22);  minor base G (0/1);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CTGAAAGTCGAGCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGC  >  NC_000913/22700‑23183                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               cTGAAAGTCGAGCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTg                                                                                                                                                                                                                                           >  1:484539/1‑251 (MQ=255)         cGAGCAAGAAGACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTg                                                                                                                                                                                                                                   <  1:19350/251‑1 (MQ=255)           aGCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGCACACTGGTGCGTTGAc                                                                                                                                                                                                                                 >  2:239649/1‑251 (MQ=255)                      ggTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGCAGCCGGTACACTGGTGCGTTGACTGCCGTTCTgc                                                                                                                                                                                                                      >  2:197707/1‑251 (MQ=255)                          agccgggtaggaaagacACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTg                                                                                                                                                                                                                  <  2:64111/235‑1 (MQ=255)                            ccGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGgc                                                                                                                                                                                                                >  2:254483/1‑251 (MQ=255)                            ccGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGgc                                                                                                                                                                                                                <  2:201848/251‑1 (MQ=255)                             cGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGgcg                                                                                                                                                                                                               >  1:235900/1‑251 (MQ=255)                                               ccgccgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCCTCCGCGCGCTGGGCAACATCATCGGCAACGGTCACCTGCCCAACGGCGCGAAGCCGGTACCCTGGTGCGTTGACTGCCGTTCTTCCCTGGCGGACACGGAAGTTggtgtt                                                                                                                                                                                             >  2:6549/1‑246 (MQ=255)                                               ccgccgAGTCCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTAGCCGTCTGGGCGTGCTGGGCGACTGGTCGCCCCCGTACCTGACCAGGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTAt                                                                                                                                                                                             <  2:433147/251‑1 (MQ=255)                                                cgccgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGCCCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACAGCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTAtt                                                                                                                                                                                            <  2:200884/251‑1 (MQ=255)                                                   cgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACg                                                                                                                                                                                         <  1:473990/251‑1 (MQ=255)                                                         ccGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACaaa                                                                                                                                                                                    >  1:186319/1‑250 (MQ=255)                                                                acgcgCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACAGCATCCGCGCGCTGGGCAAAATCAGCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTAGTACGACAAAACTTCTcc                                                                                                                                                                            <  2:248020/247‑1 (MQ=255)                                                                aaGTGCCGCGAATACGAGGCCACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGCCCATGGACTTCAAAACTGAAGCAAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAACGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGACGGAAGCGGAAGTTGGGTATTACGCCAAATCTTCTcc                                                                                                                                                                            >  2:455223/1‑251 (MQ=255)                                                                 aGTGCCGTGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCCGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTTACCATGGACTTCAAAACTGAAGCCAACATCAGCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTTGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCg                                                                                                                                                                           <  2:31876/251‑1 (MQ=255)                                                                        cgAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGGCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCg                                                                                                                                                                    <  2:235900/251‑1 (MQ=255)                                                                           aTACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACg                                                                                                                                                                 <  1:364180/251‑1 (MQ=255)                                                                                 ggcgACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAACGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAACCTTCTCCGACCCTCGCCGTTGCtt                                                                                                                                                           >  2:345911/1‑251 (MQ=255)                                                                                         aGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGcg                                                                                                                                                   >  1:88010/1‑250 (MQ=255)                                                                                           gTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGcggt                                                                                                                                                 <  1:305248/251‑4 (MQ=255)                                                                                             tGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTc                                                                                                                                                <  1:46954/250‑1 (MQ=255)                                                                                               aCGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGAt                                                                                                                                             <  1:34155/251‑1 (MQ=255)                                                                                                         cAAAGACTTTATCCGGCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCAAACATCATCCGCGCGCAGGGCAAACTAATCGGCACCGGTCACCTGCCCAAAGGCGCGAAGCCGGTACACTGGTGCGTTGCCTGCCGTTCTACGCTGGCGGAAGCGGACGTTGAGTAATAAGACAAAACTTCTCCGTCCATCGACGTAGCTTTTCAGGCGGACGATCAGGaagcc                                                                                                                                    >  2:352058/1‑246 (MQ=255)                                                                                                             gccTTTAGCCGTCTGGGCGTGCGGGGCGACTGGTCGCACCCGTACCTGACCATGGACGTCAAAACTGAAGCCAACATCAGCCGCGCGCTGGGCAAAATCAGCGGCAACGGTCACCTGCACAAAGGGGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTGCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTGTCAGGCGGTCGATCAGGATGCACTGaa                                                                                                                               <  2:196439/249‑1 (MQ=255)                                                                                                              aCTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGACCAGGATGCACTGaa                                                                                                                               >  1:264489/1‑250 (MQ=255)                                                                                                                      cGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAAtt                                                                                                                      >  1:209806/1‑251 (MQ=255)                                                                                                                              cGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTa                                                                                                               >  1:346915/1‑250 (MQ=255)                                                                                                                                            ggtccaaccctgaccataCCATGGTCTTCACACCTGAAGCCAACATCCTCCGCGCGCTGGGCAAAATCATCGGCAACGGGCACCTGCACAAATGCGCGAAGCCGGTGCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAATCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCACCGACGTTGCGTTTCAGGCGGTCGAGCAGGATGCACTGAAAGAAAAATTTGCCGTAAGCAACGTTAACg                                                                                                  <  2:202698/232‑1 (MQ=255)                                                                                                                                               cGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCa                                                                                             <  1:309558/251‑1 (MQ=255)                                                                                                                                                gCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCa                                                                                             >  1:79114/1‑250 (MQ=255)                                                                                                                                                         ccTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCGGGGCAAAATCATCGGCAACGGGCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTgg                                                                                   <  2:88010/251‑1 (MQ=255)                                                                                                                                                             aCCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGACCCTCGACGTTGCTTTTCAGACGGTCGATCAGGATGCACTGAAAGACAAATTTGCCGTAAGCAACGTTAACGGCCACATCTCGCTGGtgat                                                                               >  2:31070/1‑248 (MQ=255)                                                                                                                                                                 tGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTCCGACAAAACTTCTCCGTCCATCGACGCTGCTTTTCAGGCGGTCGATCAGGATGCCCTGAAAGCACAATTTGCCGTAATCACCGTTAACGGCCAAATCTCGCTGGTAAttagg                                                                           >  2:154179/1‑247 (MQ=255)                                                                                                                                                                    aCTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGCAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTTGacc                                                                        >  1:199055/1‑251 (MQ=255)                                                                                                                                                                      ttCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGACGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCCCTGCAAACAAAATTTGCAGTAAGCAACATTAACGGCCCATTCTCGcgggggatctgggcccc                                                                      >  2:256491/1‑235 (MQ=255)                                                                                                                                                                                aaGCCAACAACATCCGCGCGCTGGGCAAAATCAGCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTgg                                                            <  2:484539/251‑1 (MQ=255)                                                                                                                                                                                 aGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTgg                                                            >  1:5974/1‑250 (MQ=255)                                                                                                                                                                                  gCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCAGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAAAGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGAc                                                          >  1:12686/1‑251 (MQ=255)                                                                                                                                                                                     atcaccatcCGCGCGCTGGGCAAAATCCTCGGAAACGGTAACCTGCAAAAAGGCGAGAAGCCGGTAAATGGGTGCGTTGACTGCCGTGCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGActct                                                       <  2:422941/246‑1 (MQ=255)                                                                                                                                                                                     aaCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGCCAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGActct                                                       >  1:298042/1‑251 (MQ=255)                                                                                                                                                                                        atcatcCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTcagcc                                                    >  1:242823/1‑251 (MQ=255)                                                                                                                                                                                                cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTctgcctgcgaccc                                            >  1:103800/1‑246 (MQ=255)                                                                                                                                                                                                     tGGGCAAAATCATCGGCAACGGTCTCCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCgcgc                                        >  1:123181/1‑250 (MQ=255)                                                                                                                                                                                                            aaTCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTa                                <  1:239649/251‑1 (MQ=255)                                                                                                                                                                                                             atcatcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTAt                               <  1:227793/251‑1 (MQ=255)                                                                                                                                                                                                                atcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGc                            >  1:143641/1‑251 (MQ=255)                                                                                                                                                                                                                  cGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAc                          >  1:296228/1‑251 (MQ=255)                                                                                                                                                                                                                    gCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAcct                        <  1:394655/251‑2 (MQ=255)                                                                                                                                                                                                                         ggTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAttt                   >  1:117997/1‑251 (MQ=255)                                                                                                                                                                                                                         ggTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGGTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAttt                   >  2:98103/1‑251 (MQ=255)                                                                                                                                                                                                                                    caAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTctgcc                                                    <  2:12921/207‑1 (MQ=255)                                                                                                                                                                                                                                    caAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTctgcc                                                    >  1:12921/1‑207 (MQ=255)                                                                                                                                                                                                                                         gcgcGAAGACGGTACACTGGTGCGTTGACTGCCGTCCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAACACAAAATTTGCCGTAAGCAACGTTAAAGGCCCAGTCTCGCTGGTGATCTGTCCCCCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCAATTTCCCCTGCTTTCGACTATGCGCTGGTg   >  2:32458/1‑251 (MQ=255)                                                                                                                                                                                                                                            cgAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGCCTATGCGCTGGTGc  >  1:89218/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               CTGAAAGTCGAGCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGC  >  NC_000913/22700‑23183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑