Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	22,048	G→A	L214L (CTG→CTA)	ribF →	bifunctional riboflavin kinase/FMN adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	22,048	0	G	A	100.0%	132.8 / NA	46	L214L (CTG→CTA)	ribF	bifunctional riboflavin kinase/FMN adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/24);  total (22/24)

TGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCGCCGTGCGTCAGGCCCTTGCGGATGACAATCTGGCTCTGGCAGAGAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTGCGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGC  >  NC_000913/21805‑22289                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   tGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTc                                                                                                                                                                                                                                             >  1:482113/1‑251 (MQ=255)  gAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTc                                                                                                                                                                                                                                             <  1:459645/250‑1 (MQ=255)        gATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTc                                                                                                                                                                                                                                      >  2:289930/1‑251 (MQ=255)                     taCAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAggg                                                                                                                                                                                                                         >  2:238521/1‑251 (MQ=255)                            aGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATg                                                                                                                                                                                                                  <  1:106469/251‑1 (MQ=255)                            aGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACAGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATg                                                                                                                                                                                                                  <  1:473276/251‑1 (MQ=255)                                    aggAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGCATGTACCGCTACGCCGTCAGGTTTCCCCGGGGAAAGGGGTTTATGCGGTAGa                                                                                                                                                                                                           >  2:259910/2‑250 (MQ=255)                                           cGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTgg                                                                                                                                                                                                   <  1:289930/251‑1 (MQ=255)                                                       cACCAGTACGCAAACTGTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCTCGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGaa                                                                                                                                                                                        <  2:418352/250‑1 (MQ=255)                                                       cACCAGTACGCAAAATTTTGGCGAATGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCAATATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCGGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGGGCTGGGCCTCGGTGaa                                                                                                                                                                                        >  1:335832/1‑250 (MQ=255)                                                                            cGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGCAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCa                                                                                                                                                                   >  2:65425/1‑250 (MQ=255)                                                                               aGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACa                                                                                                                                                               <  1:296916/251‑1 (MQ=255)                                                                                  tGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAAAGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTGTGAGGTAGAAGTGATGGGCCTCGTTGAAAAGCCGTTACCAGGCGTGGCAAGCATCg                                                                                                                                                            >  1:324678/1‑251 (MQ=255)                                                                                       tGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAac                                                                                                                                                        >  1:105798/1‑250 (MQ=255)                                                                                                  agcaCCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCCGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCACGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGCACACGCCCAACGGt                                                                                                                                            >  2:50679/1‑251 (MQ=255)                                                                                                   gcaCCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAACGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTCGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAACCCTCGGAACACGCCCAACGGtt                                                                                                                                           >  2:225277/1‑251 (MQ=255)                                                                                                     aCCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGGGAAAGGTGTTTATGCGGTAGAAGTGCTGGGCCCCGGTGAAAAGCCGTTAACAGGCGTGGCAAACATCGGAACACGCCAAACGGttac                                                                                                                                         >  2:371140/1‑249 (MQ=255)                                                                                                      ccgctgTGCGTCAGTCTCTGGCGGATGACAATCTGGCTCGTGCAGAAAGTTTACTGGGGCCACCGTTTGGCATCACAGGGCGTGTAGTCCACGGTTATGAATTATGGCGCTCTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑cc                                                                                                                                       <  2:188186/251‑1 (MQ=255)                                                                                                       cgctgTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCg                                                                                                                                      <  1:238521/247‑1 (MQ=255)                                                                                                       cgctgTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCg                                                                                                                                      >  1:432184/5‑251 (MQ=255)                                                                                                          tgTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGCAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCACCGGTTG‑CCGGt                                                                                                                                    >  2:399619/2‑250 (MQ=255)                                                                                                             gCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTc                                                                                                                                <  1:65425/251‑1 (MQ=255)                                                                                                                 gaggagctggCGGATGACAATCTGGCTCAGGCAGAAAGGTTACTGGGGCACCCGTGTGCCAGCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTAGTCGCca                                                                                                                            <  2:99656/242‑1 (MQ=255)                                                                                                                        gggCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTg                                                                                                                     <  1:5496/249‑1 (MQ=255)                                                                                                                         ggCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGTTGATGAATTAGGGCGCACTATAGGTTGCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTgg                                                                                                                    <  2:145698/250‑1 (MQ=255)                                                                                                                              aTGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTg                                                                                                               <  1:166444/251‑1 (MQ=255)                                                                                                                                   aaTCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCAttt                                                                                                          <  1:136244/251‑1 (MQ=255)                                                                                                                                      gtggcgtgtgcAGAAAGTTTACGGGGGCCCCGGTTTGCTATCTCCGGGCGTGTGGTCCACGGTGATGAATTAGGGCGCCCTATAGGTTTCCCGACGGCGAATGTACCGCCACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGt                                                                                                        <  2:148316/241‑1 (MQ=255)                                                                                                                                                    aagTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCCTTTGtt                                                                                                       >  2:117388/2‑237 (MQ=255)                                                                                                                                                    aagTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGtt                                                                                                       <  1:117388/236‑1 (MQ=255)                                                                                                                                                         tACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCtt                                                                                    >  1:201575/1‑251 (MQ=255)                                                                                                                                                                gcacctggttGCCATCTCCGGGCGTGTAGTCCACGTTGATGAATTAGGGCGCACTATAGGTTTCCCGACTGCGATTGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTTCCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTc                                                                             <  2:253698/243‑1 (MQ=255)                                                                                                                                                                                       gtgtAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGTCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACAGCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTGGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGt                                                      <  1:288173/251‑1 (MQ=255)                                                                                                                                                                                            gTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTaaaaa                                                 <  2:432184/251‑1 (MQ=255)                                                                                                                                                                                             tCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGAGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCGGCGTaaaaa                                                 >  2:353924/1‑250 (MQ=255)                                                                                                                                                                                                aCGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGt                                                                                                                                    >  2:233392/1‑164 (MQ=255)                                                                                                                                                                                                aCGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGt                                                                                                                                    <  1:233392/164‑1 (MQ=255)                                                                                                                                                                                                         aaTTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATg                                      >  1:368928/1‑249 (MQ=255)                                                                                                                                                                                                                  gcACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCACACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCATTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGAttt                           >  1:461781/1‑251 (MQ=255)                                                                                                                                                                                                                       ataGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGt                       <  2:356996/250‑1 (MQ=255)                                                                                                                                                                                                                          ggttgtccGACGGCGAATGTGCCGGTACGCCGACAGGTGGCCCCGGTGAAAGGGGGTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACAGCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCAATTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAAGGAGCAGCGATTTGCGTCGCt                   <  2:132232/245‑1 (MQ=255)                                                                                                                                                                                                                               cccGTCGGCGAATGTACCGCTACGCCGGCAGGTTTCCCCGGTGAAAGGGGTGTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGAc               <  1:462530/250‑1 (MQ=255)                                                                                                                                                                                                                                  gccGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGAGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGGATTCGCCAGCAGCTAGAAGTGCATTTGTACGATGTTGCAATGGACCTTTACGGTCGCCATAAACAAGTAGTGCGGGGTAAAAAAATACGAAATGAGCAGCGATTTGCGTCGAGGGACGaa            >  2:265413/3‑250 (MQ=255)                                                                                                                                                                                                                                    gggtggaTGTGCCGCTACGCCGTCAGGTTGCCCCGGTGAAAGGGGGTTAGGCGGGAGAAGTGCTGGGCCGCGGCGAAAAGCCGTTACGCGGCGTGGCAAACATCGGAGCACGCCCAA‑GGTTGGCCAGTATTCGCGAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGCCCTGTACGGGTGCCATATACAAGTAGGGCTGCGTAAAAAAATACGCAAGGAGCAGCGATTTGCGTCGCTGGACGAACTg         <  1:26501/245‑1 (MQ=255)                                                                                                                                                                                                                                           tGTACCGCTACGACGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTCGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAgcgc  >  1:422056/1‑251 (MQ=255)                                                                                                                                                                                                                                               ccGCTACGCCGTCAGGTTTCCCCGGTGAACGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGCTGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGCACTGAAAgcgc  >  1:258524/1‑247 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   TGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCGCCGTGCGTCAGGCCCTTGCGGATGACAATCTGGCTCTGGCAGAGAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTGCGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTG‑CCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGC  >  NC_000913/21805‑22289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑