Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	19,715	G→A	intergenic (+95/+96)	nhaR → / ← insB1	DNA‑binding transcriptional activator NhaR/IS1 family protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	19,715	0	G	A	100.0%	29.8 / NA	12	intergenic (+95/+96)	nhaR/insB1	DNA‑binding transcriptional activator NhaR/IS1 family protein InsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

AAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCC‑CCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA  >  NC_000913/19477‑19819                                                                                                                                                                                                                                                 |                                                                                                         aaaaCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGt                                                                                               >  1:371088/1‑251 (MQ=255)   aacggtcggataaatgtgttgcggcGAGAATGTGAAGGAAGAGTACCAGTCCATTTTTGCTGAGCGGATTATTCAGCACCCGGCGGTTCAGCCAATCCGCAATACGGATTATTCTGCGCTTTTTAGTCCCTCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc                                                                                             <  1:450339/227‑1 (MQ=255)                        cgTCGAGAATGTGCTGGAAGAGTACCATGCTATTTTTGATGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTTTTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTTGGGGAGAGAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATagagtaa                                                                        <  2:303957/251‑4 (MQ=255)                                                  aTGCTATTTTTGCTGAGCGGATGATTCAGCACCCGTCGGAACAGCGAATCTGCAATTCGGAGTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCACAC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACCTAACAACAATTTTGAATCGGCTCATTATGTGCCCGCTTGAGACATTATTTCCGTATGTACGCGGGCGAATAAATAGAGGAATATGTTTAATTAATTAATGGGGATgg                                              >  2:478542/1‑250 (MQ=255)                                                  aTGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGCATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTACTCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGCTAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGGGGAATCTGATTACTTCCTTCATGGGGATgt                                              >  2:41818/1‑250 (MQ=255)                                                                 ggCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTaa                               <  2:13394/250‑1 (MQ=255)                                                                          cccAGCACCCGGCGGTACAGCGAAGCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGGTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCtttt                       <  2:243994/248‑4 (MQ=255)                                                                            cAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGCGTAGTAATTGCtttt                       >  1:243994/1‑245 (MQ=255)                                                                               cACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccata                 <  1:344642/251‑10 (MQ=255)                                                                                               gAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATAGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTAATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAATTAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccctactggatttcattcga  >  2:462599/1‑226 (MQ=255)                                                                                                 aTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGAt                                                >  1:93422/1‑202 (MQ=255)                                                                                                 aTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGAt                                                <  2:93422/202‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                         AAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCC‑CCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA  >  NC_000913/19477‑19819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑