Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 19,670 T→A intergenic (+50/+141) nhaR → / ← insB1 DNA‑binding transcriptional activator NhaR/IS1 family protein InsB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091319,6700TA90.5% 59.3 / ‑0.2 22intergenic (+50/+141)nhaR/insB1DNA‑binding transcriptional activator NhaR/IS1 family protein InsB
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (9/10);  total (10/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

TCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA  >  NC_000913/19436‑19819
                                                                                                                                                                                                                                            |                                                                                                                                                     
tCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCTATAAAACTGTCGTAGAAATTGGTCGCGTCGTGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTGAGTACAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATCACCACGATTTTGAc                                                                                                                                         <  2:96919/250‑1 (MQ=255)
  gTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTCAGGTGGGGGAGATAGATTAGTTGTACATAACCACGTTTTTGACTCg                                                                                                                                      >  2:189504/1‑251 (MQ=255)
          aaCGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCatta                                                                                                                              <  1:189504/251‑1 (MQ=255)
          aaCGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCatt                                                                                                                               >  1:294716/1‑250 (MQ=255)
                    gCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGGCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCt                                                                                                                    <  2:294716/251‑1 (MQ=255)
                     cATATGGCGTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTATTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCtt                                                                                                                   <  1:122051/251‑1 (MQ=255)
                                        tAAAACTGTCGTAGAAATTGATCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTg                                                                                                                                           >  1:126755/1‑207 (MQ=255)
                                        tAAAACTGTCGTAGAAATTGATCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTg                                                                                                                                           <  2:126755/207‑1 (MQ=255)
                                         aaaaCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGt                                                                                               >  1:371088/1‑251 (MQ=255)
                                           aacggtcggataaatgtgttgcggcGAGAATGTGAAGGAAGAGTACCAGTCCATTTTTGCTGAGCGGATTATTCAGCACCCGGCGGTTCAGCCAATCCGCAATACGGATTATTCTGCGCTTTTTAGTCCCTCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc                                                                                             <  1:450339/227‑1 (MQ=255)
                                                                cgTCGAGAATGTGCTGGAAGAGTACCATGCTATTTTTGATGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTTTTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTTGGGGAGAGAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATagagtaa                                                                        <  2:303957/251‑4 (MQ=255)
                                                                            gATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGc                                                                                                                         >  1:63034/1‑190 (MQ=255)
                                                                            gATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGc                                                                                                                         <  2:63034/190‑1 (MQ=255)
                                                                                          aTGCTATTTTTGCTGAGCGGATGATTCAGCACCCGTCGGAACAGCGAATCTGCAATTCGGAGTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCACACAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACCTAACAACAATTTTGAATCGGCTCATTATGTGCCCGCTTGAGACATTATTTCCGTATGTACGCGGGCGAATAAATAGAGGAATATGTTTAATTAATTAATGGGGATgg                                              >  2:478542/1‑250 (MQ=255)
                                                                                          aTGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGCATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTACTCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGCTAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGGGGAATCTGATTACTTCCTTCATGGGGATgt                                              >  2:41818/1‑250 (MQ=255)
                                                                                                         ggCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTaa                               <  2:13394/250‑1 (MQ=255)
                                                                                                                  cccAGCACCCGGCGGTACAGCGAAGCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGGTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCtttt                       <  2:243994/248‑4 (MQ=255)
                                                                                                                    cAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGCGTAGTAATTGCtttt                       >  1:243994/1‑245 (MQ=255)
                                                                                                                       cACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccata                 <  1:344642/251‑10 (MQ=255)
                                                                                                                                       gAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATAGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTAATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAATTAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccctactggatttcattcga  >  2:462599/1‑226 (MQ=255)
                                                                                                                                         aTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGAt                                                >  1:93422/1‑202 (MQ=255)
                                                                                                                                         aTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGAt                                                <  2:93422/202‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                     
TCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA  >  NC_000913/19436‑19819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: