| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 19,613 | T→C | V300A (GTG→GCG) | nhaR → | DNA‑binding transcriptional activator NhaR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 19,613 | 0 | T | C | 93.5% | 81.3 / ‑1.6 | 31 | V300A (GTG→GCG) | nhaR | DNA‑binding transcriptional activator NhaR |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (11/18); total (12/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.16e-01 | |||||||||||
GTAGAAATCCTCGGCGAGTTTGATGATGCCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCC‑CCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA > NC_000913/19366‑19819 | gTAGAAATCCTCGGCGAGTTTGATGATGCCGCGTTGATGACAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGAAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACAATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTTCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGcgcg > 2:468193/1‑251 (MQ=255) aaTCCTCGGCGAGTTTGATGATGCCTCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAAt < 1:259575/251‑1 (MQ=255) aaTCCTCGGCGAGTTTGATGATGCCGCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAAt < 1:397177/251‑1 (MQ=255) ccTCGGCGAGTTTGATGATGCCGCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCg < 1:1000/250‑1 (MQ=255) ccGGCGAGGTTGATGATGCCGCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCa < 2:186635/250‑1 (MQ=255) cgttGATGAAAGCTTTTGGTGCGATGCACAATGCAAGCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTg < 1:468193/249‑1 (MQ=255) tCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCTATAAAACTGTCGTAGAAATTGGTCGCGTCGTGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTGAGTACAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATCACCACGATTTTGAc < 2:96919/250‑1 (MQ=255) gTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTCAGGTGGGGGAGATAGATTAGTTGTACATAACCACGTTTTTGACTCg > 2:189504/1‑251 (MQ=255) aaCGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCatta < 1:189504/251‑1 (MQ=255) aaCGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCatt > 1:294716/1‑250 (MQ=255) cTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGAtt > 1:71588/1‑211 (MQ=255) cTTTATGCAGATGACTTGTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGAtt < 2:71588/211‑1 (MQ=255) gCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGGCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCt < 2:294716/251‑1 (MQ=255) cATATGGCGTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTATTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCtt < 1:122051/251‑1 (MQ=255) tAAAACTGTCGTAGAAATTGATCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCC‑CCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTg > 1:126755/1‑207 (MQ=255) tAAAACTGTCGTAGAAATTGATCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCC‑CCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTg < 2:126755/207‑1 (MQ=255) aaaaCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGt > 1:371088/1‑251 (MQ=255) aacggtcggataaatgtgttgcggcGAGAATGTGAAGGAAGAGTACCAGTCCATTTTTGCTGAGCGGATTATTCAGCACCCGGCGGTTCAGCCAATCCGCAATACGGATTATTCTGCGCTTTTTAGTCCCTCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc < 1:450339/227‑1 (MQ=255) cgTCGAGAATGTGCTGGAAGAGTACCATGCTATTTTTGATGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTTTTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTTGGGGAGAGAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATagagtaa < 2:303957/251‑4 (MQ=255) cgTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTg < 1:468098/166‑1 (MQ=255) cgTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTg > 2:468098/1‑166 (MQ=255) gATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGc < 2:63034/190‑1 (MQ=255) gATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGc > 1:63034/1‑190 (MQ=255) aTGCTATTTTTGCTGAGCGGATGATTCAGCACCCGTCGGAACAGCGAATCTGCAATTCGGAGTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCACAC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACCTAACAACAATTTTGAATCGGCTCATTATGTGCCCGCTTGAGACATTATTTCCGTATGTACGCGGGCGAATAAATAGAGGAATATGTTTAATTAATTAATGGGGATgg > 2:478542/1‑250 (MQ=255) aTGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGCATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTACTCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGCTAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGGGGAATCTGATTACTTCCTTCATGGGGATgt > 2:41818/1‑250 (MQ=255) ggCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTaa < 2:13394/250‑1 (MQ=255) cccAGCACCCGGCGGTACAGCGAAGCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGGTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCtttt < 2:243994/248‑4 (MQ=255) cAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGCGTAGTAATTGCtttt > 1:243994/1‑245 (MQ=255) cACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccata < 1:344642/251‑10 (MQ=255) gAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATAGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTAATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAATTAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccctactggatttcattcga > 2:462599/1‑226 (MQ=255) aTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGAt < 2:93422/202‑1 (MQ=255) aTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCC‑CCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGAt > 1:93422/1‑202 (MQ=255) | GTAGAAATCCTCGGCGAGTTTGATGATGCCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCC‑CCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA > NC_000913/19366‑19819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |