Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	393	T→G	R19R (CGT→CGG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	393	0	T	G	96.8%	81.2 / NA	31	R19R (CGT→CGG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (16/14);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAAT  >  NC_000913/152‑628                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            cgcACAGCCAGATAAAAATTACAGAGTACACAACATCCCTGACACGCATTAGCACCACCATTACAACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGACACACCGAACAAAGCCCGCACCTGACGGTACGGGCTTTTTTTT‑‑CGCCCATAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGGGGCAAATGCCGAACGTTTTATGCGGGTTgacgcg                                                                                                                                                                                                                                    >  2:290394/1‑245 (MQ=255)     cagacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCt                                                                                                                                                                                                                               <  1:201965/251‑1 (MQ=255)                   ttACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGcc                                                                                                                                                                                                                  >  2:374749/1‑250 (MQ=255)                         agTACACAACAACCATGAACCGCATTAGCACCCCCATTACCACCACCAGCTCCATTTCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACTCAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑‑CGACCAAAGTTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTGGCCGATATTCTGGAAAGCAATGCcaggcagg                                                                                                                                                                                                           <  1:290394/251‑1 (MQ=255)                                  caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcaggggg                                                                                                                                                                                                  >  1:328225/1‑248 (MQ=255)                                           aaCGCATTAGCACCACAATTACCACCACCATCACCATTACCACAGGTACCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAACAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTTGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAACAATGCCAGGCCGGGGGAGGTGGCCCCCggcc                                                                                                                                                                                         >  2:292692/1‑248 (MQ=255)                                                                          caccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCt                                                                                                                                                          >  1:428694/1‑251 (MQ=255)                                                                           accatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑‑CGACCAAAGGTAACGAGGAAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCCGGGGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCtg                                                                                                                                                         >  2:173995/1‑251 (MQ=255)                                                                                      gggTAAGGGGGCGGGCTGACGCGTAAAGGAAACACAAAAAAAAGCCCGCGCCTGACAGTG‑GGGTCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTCCATCAGGGGCAAATGCAGAACGTTTTCTGCGGGTGGCGGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACGGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCgatgat                                                                                                                                              <  2:477668/250‑1 (MQ=255)                                                                                      agggaaCGGGGCGGGCTGACGCGTAAAGGAAACACAGAAAAAGGCCCGCACATGACAGGGCGGGCTTTTTTTTTTCGACCAAAGGTAACGAGGTAAAAACCATGCGAGTGTTGAAGTTCGGCGGAAAATAAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGTCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCTCCTGGTGGCgatg                                                                                                                                                <  1:102124/247‑1 (MQ=255)                                                                                              gggcgggctgagtcgaaaagaaaaCACAGAAAAAAGCCCGTACCTGACAGGGGGGGCTTTTTTTT‑‑CGACCAAAGGTAACGGGGTAACAATCATGCGAGTGTGGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTGGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCACTACCCCCGCCAAAATCACCAACCTCCTGGTGGCGATGATTGaaaaaa                                                                                                                                      <  2:326432/230‑1 (MQ=255)                                                                                                      tGACGCGTAAAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTAAATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTACTTTCTGCCCCCGCCCAAATCACCAACCACCTGGGGGCGATGATTGGAAAAAACAATAGc                                                                                                                              >  2:202978/1‑251 (MQ=255)                                                                                                         cgggTACAGGAAACACAAAAAAAAGCCCGCCACTGAAAGTGCGGG‑TTTTTTTTC‑CGAACAAAGGTAACGGGGTAACACCCAGGTGAGTGTTGAAGTTCGGCGGTACATCAGTGGAAAAGGCAGAACGTTTTCTGCGGGTTCCAGATATTCGGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGc                                                                                                                           <  2:418897/248‑1 (MQ=255)                                                                                                               cAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑‑CGACCAAAGGTAACGAGGTACCAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCACCCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGAt                                                                                                                     <  1:374749/251‑1 (MQ=255)                                                                                                               aagaaaaCAAAGAAAAAAGCCCGCACCTGCCATTGCGGGCTTTTTGTC‑‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGAAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAAGGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGAt                                                                                                                     <  2:303079/247‑1 (MQ=255)                                                                                                                                    cGCACCTGACAGTGCGGGCTTTTTTTT‑‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGAt                                                                                                >  1:300367/1‑251 (MQ=255)                                                                                                                                      cACCTGACAGTGCGGGCTTTTTTTT‑‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGc                                                                                              >  1:289801/1‑251 (MQ=255)                                                                                                                                                        tttttttt‑CGACCAACGGTAACGAGGTAACAACCATGCGAGCGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCCGGTGGCCACCGTCCTCTCTGCCCCCGCCCAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGAATTTTTGCCg                                                                             >  1:339541/1‑251 (MQ=255)                                                                                                                                                         ttttttt‑CGACCAAAGGTAACGAGGTAACAACCATGCGCGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGCACGTATTTTTGCCg                                                                             >  1:132958/1‑250 (MQ=255)                                                                                                                                                                     cAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAAACATTAGCGGCCAGGATGCTTTa                                                                                                               >  1:96592/1‑205 (MQ=255)                                                                                                                                                                     cAAAGGTAAAGAGTTAAAAACCATGCGAGTGTTGAATTTCGGCGTGACATCAGTGGCAAATGCAGAACGGTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCttga                                                                                                               <  2:96592/205‑3 (MQ=255)                                                                                                                                                                      aaaggaaacgggtaaacaacCATGGGAGTGTTGAAGTTCGGCGGTACACCAGTGGAAAATGCAGTACGTTTTTGGCGGGTTGCCGATATTCTGGAAAGCAAGGCCAGGCAGGGGAAGGTGGCCCCCGGCCTCTCTGCCCCCGCCAAAATCACCAATCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACggg                                                                <  2:428694/237‑1 (MQ=255)                                                                                                                                                                      aaaGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACggg                                                                >  1:177878/1‑251 (MQ=255)                                                                                                                                                                            aaCGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTcgc                                                          <  1:173995/251‑1 (MQ=255)                                                                                                                                                                                                 gtgtTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCgggat                                         >  1:54382/1‑245 (MQ=255)                                                                                                                                                                                                 gtgtTGAAGTCCGGCGGGAAAACAGGGGCAAATGCAGAACGTTTTCGGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCCCCGGCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCAATAGCGGCCAGGATGCTTTACCCAATAGCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCgggat                                         <  2:54382/247‑3 (MQ=255)                                                                                                                                                                                                                       aaggggCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATCCGGGAAAGCAATGCCAGGCAGGGGCGGGTGGCCCCCGTCCTCCCTGCCCCCGCCAAAAGCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACttgc               <  2:289801/247‑3 (MQ=255)                                                                                                                                                                                                                           ggCAAATTCAAAACTTTTTCTGCGGGTTCCCGATATTCTGGAAAGCATTGCCAGGCAGGGGCAGTTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGTATTCCCGCTGGCGCTATTGAAAACTTtcgttg           <  2:337889/251‑3 (MQ=255)                                                                                                                                                                                                                            gCAAAGGCAGAACGTTTCCTGCGGGTTGCCGATATGCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGa          <  1:222143/251‑1 (MQ=255)                                                                                                                                                                                                                                 tGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGAAGCCAAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCACCCAGCCGGGATTCCCGCTGGCGCCAtt                         >  1:74543/1‑231 (MQ=255)                                                                                                                                                                                                                                    aGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAAt  >  1:97414/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            CGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAAT  >  NC_000913/152‑628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑