Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 17,219 G→T intergenic (+209/‑270) sokC → / → nhaA small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091317,2190GT91.7% 67.2 / ‑5.4 25intergenic (+209/‑270)sokC/nhaAsmall regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base T (14/8);  minor base A (1/1);  total (16/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACG  >  NC_000913/16993‑17460
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  
cTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGac                                                                                                                                                                                                                             >  1:13292/1‑250 (MQ=255)
                          cAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGACCTACCAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCtaat                                                                                                                                                                                                  >  1:337829/1‑251 (MQ=255)
                             cTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCtaatta                                                                                                                                                                                                >  1:205316/1‑250 (MQ=255)
                                                                   attatATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCtgg                                                                                                                                                         <  2:378184/251‑3 (MQ=255)
                                                                                 tttAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTc                                                                                                                                           >  1:224670/1‑251 (MQ=255)
                                                                                                     cGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGGGAGCGCTTACAGCCGTc                                                                                                                        >  2:256134/1‑250 (MQ=255)
                                                                                                       aTTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCaaa                                                                                                                     <  2:99925/251‑1 (MQ=255)
                                                                                                                                     gtgGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAAt                                                                                        <  2:179370/250‑1 (MQ=255)
                                                                                                                                                 tttAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCTATTATTGTCGACTAGCTGGATTATTTTTGTATTATTGTCCTAACAAATAACGCCGACTGACAAGAATTTAATTATTACTTTTACAAATTAATCCATCAGAAATCCTCACTTTAAGCTATGTTTATCTGTGTTTAGGGTCAATCGTGAGCGCTTACAGCAGTCAAAAATCTGATCTAACAGCTGTTGGAGCAAATTCTTCactggagc                                                                           >  2:18780/1‑244 (MQ=255)
                                                                                                                                                    aaTGATTTAATTATTTAACTTTAATTTATCTCTTAATCGCAATTATTGTCTACAATCTGGATTATTATTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATGAAGCCCTCAGAAATCCTCACCTTAAGCGGTGATAATCTGGACTAAGGGGCACTCGGGAGCGCTTACAGCCTTCAAAAATCTCAGCGCACCGCTGATGGCGCAAATTCGTCAAtggacctt                                                                         <  2:471609/250‑8 (MQ=255)
                                                                                                                                                       gATTTAATTATTTAACTTTAATTTATCTATTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTaaa                                                                      >  1:179382/1‑250 (MQ=255)
                                                                                                                                                          ttAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAAc                                                                  >  1:412681/1‑251 (MQ=255)
                                                                                                                                                               tattTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGagt                                                              <  2:369855/250‑3 (MQ=255)
                                                                                                                                                                        ttAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTCCTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTac                                                     <  1:256134/250‑1 (MQ=255)
                                                                                                                                                                             ttATCTCTTTATCGCAATTATTGACGCCAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTACTTATTACTTTTACTAATTAATCCCTCAGAACTCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAACTTCTTCAGTGGATCGTAAAAAACGAGTTATTCCTACACtat                                                >  1:477947/1‑250 (MQ=255)
                                                                                                                                                                             tgatctcattaTCGCAAGTATTGACGTCAATCTTGATTATTTTTGAAATTTTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTGCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACCCATCTCACCGCTGATGGCTCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACTCtat                                                <  2:323244/240‑1 (MQ=255)
                                                                                                                                                                                               tattGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAAAAATCCTCACCTTAAGCTATGTTTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAATTTCTTCATTGGATCGTAAAAAACGATTTATTCCTACACTATAATCTGATTTTAACgatg                              >  1:344002/1‑250 (MQ=255)
                                                                                                                                                                                                     cgacAAGCTGGATTATTTTTGAAATATTGACCTAACAAATAACGCCGACTGACAAGAAACTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTAAAGTTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTGAAATGGATCGTAAAAAACGAGTTATTCCTACTCTATAATCTGATTTTAACGATGATTtgtg                       <  2:337829/251‑1 (MQ=255)
                                                                                                                                                                                                             tGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTACTCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGATTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAACAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTa                >  1:302941/1‑250 (MQ=255)
                                                                                                                                                                                                                 ttattTTTGAAATATTGGCCTAACAAATAACGCCGCCTGACAAGCATTTAATTATTACTTTTCCTAATTAATCCCTCAGACATCCTCACCTTAAGCTATGATTATCTGGTCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGTCGCAAATTCTTCAATGTATCGTAAAAACCGAGTTATTCCTACACTATAATCTGATTTTAACGATGAtttgatcggggaaacat            >  2:185605/1‑235 (MQ=255)
                                                                                                                                                                                                                   aattttGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCTCCGTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACTCTATAACCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGt         <  1:275742/249‑1 (MQ=255)
                                                                                                                                                                                                                    tttttGAAATATTATCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGt         >  1:394672/1‑250 (MQ=255)
                                                                                                                                                                                                                       ttGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTaaa      >  1:100091/1‑250 (MQ=255)
                                                                                                                                                                                                                         gAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTACAATCGTAAAAAc   >  2:284025/1‑251 (MQ=255)
                                                                                                                                                                                                                          aaaTATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACg  >  1:71060/1‑251 (MQ=255)
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  
CTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACG  >  NC_000913/16993‑17460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑