Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,085	T→C	intergenic (+75/‑404)	sokC → / → nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,085	0	T	C	100.0%	48.6 / NA	15	intergenic (+75/‑404)	sokC/nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/6);  total (9/6)

ACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCT‑ACATGTGTTCAGCATATAGGAGGCCTCGGGTTG‑ATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTC  >  NC_000913/16848‑17324                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                aCGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCT‑ACATGTGTTCAGCATATAGGGGGCCTCGGGTTG‑ATAGTAAAATATCACTCGGGGCTTTTCTCTATCTTCCGTTCAGCTAAAGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGAtt                                                                                                                                                                                                                                    >  1:369855/1‑251 (MQ=255)                         ggggCGACAACCTCCGCTTTATGCTTCTTCTTTCCTCTCTTCCCCTTGCCTTTACGGTCAGAAAGAGCCCCTCT‑ACATGTGTACAGAATAGAGGGGGCCGGGGTTTG‑ATAAAAAAATACCCCCGGGGGTTTTTCTTTATCTCCCGTTCAGCTAATGCCTGAGACAAATACCCACAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAtt                                                                                                                                                                                                            <  1:381066/250‑1 (MQ=255)                           ggCGACAATCATCGCCTTATGCTGCTTCCTTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCT‑ACATGTGTTCAGCATATAGGGGGCCTCGGGTTG‑ATAGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAtttt                                                                                                                                                                                                          >  1:91166/1‑251 (MQ=255)                                         ccTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCT‑ACATGTGTTCAGCATATAGGGGGCCTCGGGTTG‑ATAGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAAtttt                                                                                                                                                                                            >  1:411026/1‑251 (MQ=255)                                              tgctgcTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCT‑ACATGTGTTCAGCATATAGGGGGCCTCGGGTTG‑ATAGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAAt                                                                                                                                                                                       >  1:152647/1‑251 (MQ=255)                                                          ccccttttccccTTGACTTTACGGTAAGTAAG‑GGCCCTTTTACATGTTTTCAGCATATGGGGGGCCTGGGGTTG‑ATAAAAAAATACCACTCGGGTTTTTTCTTTATCTGCCGTTCAGCTAATGCCTGAGACAGATACCCTAAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAattat                                                                                                                                                                            <  1:239557/240‑1 (MQ=255)                                                                    ccttgccctgccgggcagaaaGAGCCACTCT‑AAATGTGTTCAGCATATGGGGGGCCTCGGTTTG‑ATAGAAAAATCTCCCTGGGGGTTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACATATCGCCACAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAAt                                                                                                                                                                 <  1:11312/232‑1 (MQ=255)                                                                        gtcctgacgggaaGTAAGAGGCACTCT‑ACTTGTTTTCAGATTATGGGGGGCCTCGGTTTG‑TTAGTAAAATATCCCTCGGGGTTTTTCTCTATCTCCCGTTCATCTAATCCCGGAAACAGATTGCCCCAAGCTCCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTAt                                                                                                                                                             <  1:229718/239‑1 (MQ=255)                                                                              gtggtcataaaGAGCCACTCT‑ACATGTTTTCAGCGTATGGGGGCCCTCGG‑TTGTAGAGTAAAATATCCCTGGGGTTTTTTTTCTATCTGCCTGTCAGCTAATGTCGGAGACAGATCGCCTCAAACACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCttt                                                                                                                                                       <  1:109444/242‑2 (MQ=255)                                                                                ggTCAGTAAGAGGCACTCT‑ACATGTGTTCAGCATATAGGGGGCCTCGGGTTG‑ATAGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCCTTCTGTTTTATCGCTTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCtttat                                                                                                                                                     >  2:201977/1‑248 (MQ=255)                                                                                                                                                    cTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGac                                                                                    >  1:13292/1‑250 (MQ=255)                                                                                                                                                                              cAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGACCTACCAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCtaat                                                         >  1:337829/1‑251 (MQ=255)                                                                                                                                                                                 cTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCtaatta                                                       >  1:205316/1‑250 (MQ=255)                                                                                                                                                                                                                       attatATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCtgg                <  2:378184/251‑3 (MQ=255)                                                                                                                                                                                                                                     tttAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTc  >  1:224670/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                ACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCT‑ACATGTGTTCAGCATATAGGAGGCCTCGGGTTG‑ATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTC  >  NC_000913/16848‑17324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑