Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	15,369	T→A	intergenic (+71/‑76)	dnaJ → / → insL1	chaperone protein DnaJ/IS186/IS421 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	15,369	0	T	A	100.0%	100.3 / NA	31	intergenic (+71/‑76)	dnaJ/insL1	chaperone protein DnaJ/IS186/IS421 family transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/25);  total (6/25)

GATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCAT  >  NC_000913/15125‑15613                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     gATTTGCTGGGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTg                                                                                                                                                                                                                                                <  2:397741/251‑1 (MQ=255)                   gtcgtcGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCAt                                                                                                                                                                                                                               <  1:30496/250‑1 (MQ=255)                     cgtcgAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCggggggtactccccaaa                                                                                                                                                                                                                             >  1:239046/1‑237 (MQ=255)                      gtcgAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCgggggacacttccacacgc                                                                                                                                                                                                                           >  1:289597/1‑236 (MQ=255)                       acgAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTGCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTTCCCGTGGGCAGGCTTGGGTAAAAGTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAgct                                                                                                                                                                                                                          <  2:23449/250‑2 (MQ=255)                        cgAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCggggggtgcacccacaaaacc                                                                                                                                                                                                                         >  2:107717/1‑234 (MQ=255)                                 ggTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGGTCACTCCCCTAAACGCtcaattta                                                                                                                                                                                                                 >  1:474439/1‑243 (MQ=255)                                   gaGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAggg                                                                                                                                                                                                              <  1:128436/250‑1 (MQ=255)                                          gAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtgtg                                                                                                                                                                                                         <  1:411383/249‑1 (MQ=255)                                                                   caagagctgcaagaAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTg                                                                                                                                                                              <  1:413903/251‑1 (MQ=255)                                                                    aagagctgcaagaAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGc                                                                                                                                                                             <  1:325378/251‑1 (MQ=255)                                                                                aaaGCGTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTGGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGAGATGCGAGCGCCTGAAAAGTGGCGGGGATCACGCCCATAATCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                                                                                                                                 <  1:45970/251‑1 (MQ=38)                                                                                         ggggCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGGGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATa                                                                                                                                                        <  2:170041/249‑1 (MQ=255)                                                                                           ggCCCAACCGGTGAGCCCAACGGCCCGCTCTCTAAGAGCTTCTTTTATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCTCAAAATCCTGCCCGTGGGCAGGCTTGGTTAAAAGTATGGTGCGTTGAAGATATGCGAGCACCTGAATAGTGGCGTGGATCACTCCCATAAGCGCTAACTTAAGTGTTGTGGGATTACGCCTGATATGATTTAACGTGCTGATGAATTACTCTCACGATacc                                                                                                                                                      <  2:153326/251‑3 (MQ=255)                                                                                                aaCCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTc                                                                                                                                                 <  2:239046/251‑1 (MQ=255)                                                                                                    ggtgAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTTGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCa                                                                                                                                             <  2:289597/248‑1 (MQ=255)                                                                                                       gAGCACAACAGCCCGCGCTAAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCTCTAACTTAAGGGTTGTGGTATTACGCCTTATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt                                                                                                                                          <  1:32846/251‑1 (MQ=255)                                                                                                                 gCCCGCGCGCAAAGAGCTTCTTTGATGGTGGGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCata                                                                                                                                <  2:83873/251‑1 (MQ=255)                                                                                                                            aaGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCa                                                                                                                                                             <  2:137551/211‑1 (MQ=255)                                                                                                                               agCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGat                                                                                                                                                                                           <  2:140974/178‑1 (MQ=255)                                                                                                                                                gaagaaGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACCCCCCTAAGCGCCCACTTAAGGGGTGTGGGATTAAGCCTGGTATGATTTTACGTGCCGGTGAATTAATCTCCCCATAACTTGTTAGCAATTTTGGGCCATATTGTTAAGCCCGAAGAACTGGGTACTTccgg                                                                                                 >  1:128900/1‑248 (MQ=255)                                                                                                                                                              acgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTaa                                                                                                                         <  1:349454/213‑1 (MQ=255)                                                                                                                                                                        ccGCTAACCGTCCCAAAAGCCTGCCCGTGTGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACccg                                                                         <  1:245482/251‑1 (MQ=255)                                                                                                                                                                             aaCCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTGAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACccgccgcc                                                                    <  2:209588/251‑1 (MQ=255)                                                                                                                                                                                                         ggcttGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGGGAACTCCCAAAAAAGCTCACTTAAGGGGTGTGGGGTTATGCCTGCTATGGTTTTACGCGCCGGTGAAATAATCTCCCGATAACTGGTCAGCAAATCTGGCCCATATTTGTAAGCCCGAAGAAATGGGTAATTCGGCACGGAATGCCGGGGCTCTAACCCCCCACCGCGAAAATCGTGATGCTGCaaatcctct                                        >  1:124415/5‑244 (MQ=255)                                                                                                                                                                                                         ggcttGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACATCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGATGCAACTCTGCt                                        <  1:62895/247‑1 (MQ=255)                                                                                                                                                                                                                   aaattAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGGGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTgggg                              <  2:57386/247‑1 (MQ=255)                                                                                                                                                                                                                             gCGTTGAAGATATGCGAGGACCTGAAAAGGGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATTATTTAACGTGCCGATGAATTACTCTCTCGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTAc                     <  1:8396/250‑1 (MQ=255)                                                                                                                                                                                                                              ggTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTAAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACgg                   <  2:374534/250‑1 (MQ=255)                                                                                                                                                                                                                                           cGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGATAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATg      <  2:86519/251‑1 (MQ=255)                                                                                                                                                                                                                                               cACCTGAAAAGTGGCGGGGATCACACCCATAAGCGCGAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCAGGGGGGATGTCAt  <  2:121791/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCAT  >  NC_000913/15125‑15613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑