Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,401	C→T	G78G (GGC→GGT)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,401	0	C	T	97.8%	135.9 / ‑4.2	45	G78G (GGC→GGT)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (24/20);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-01

CAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGAC  >  NC_000913/14155‑14646                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      cAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATgg                                                                                                                                                                                                                                                   <  1:18459/251‑1 (MQ=255)              aTGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGttt                                                                                                                                                                                                                                      >  1:474215/1‑251 (MQ=255)                 gCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTgg                                                                                                                                                                                                                                   <  1:260044/251‑1 (MQ=255)                   tAAGCAAGATGATTACGAGATGTTAGGCGTTTCAAAAACAGCGGAAGAGCATGAAAGCAAAAAGGCTTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGGTCTGACCGACTCTGAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTggc                                                                                                                                                                                                                                  <  2:286641/250‑1 (MQ=255)                    aaGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTggcgg                                                                                                                                                                                                                                <  1:434161/251‑1 (MQ=255)                          gATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTAGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACATGCGGCATACGATCAGTATGGTCATGCTGCGTTGTAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGacgc                                                                                                                                                                                                                          >  2:94074/1‑251 (MQ=255)                             tattaCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAg                                                                                                                                                                                                                        <  1:196086/250‑1 (MQ=255)                                           aggcggtgccAAAACAGCGGAAGAGCATGAAAGCAAAAAGGCCTACAAACGACTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGGCGAGGCGAGAGTTAAAGAGATCAAGGAAGCATATGAAGATCTGACAGACTCGCAAAAACGGGCGGCATACGATCATGAGGGTCATGCTGCGGTTGAGCAAGGTGGTATGGGCGGCGGCTGTTTGGGCGGCGGCGCAGATTTCAGCGATAttt                                                                                                                                                                                                         <  2:409976/243‑1 (MQ=255)                                                     aaaaCAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGt                                                                                                                                                                                               <  2:162386/251‑1 (MQ=255)                                                          aGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCg                                                                                                                                                                                          <  2:340466/251‑1 (MQ=255)                                                                  agcatGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGGTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATAtt                                                                                                                                                                                  >  1:316897/5‑251 (MQ=255)                                                                               aaaGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGGGATATTTTTGGGGGCGTTTTTGGCGATATTTTTggcggggggg                                                                                                                                                                     >  2:129576/1‑246 (MQ=255)                                                                                     cgaCAAACGACGGGCCATGAAATACCCCCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGgtc                                                                                                                                                               <  2:6472/249‑1 (MQ=255)                                                                                       aCAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGgtcgt                                                                                                                                                             >  1:322697/1‑251 (MQ=255)                                                                                       aCAAACGCCTGGCCAAGAAATACCACCCGGACCGTAACCAGGGGGACAAAGAGGCCGAGGCGAAATTGAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGTGGCATACGATCAGGATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGGCTTCAGCGATATTTTTGGTGACGTTTGCGGCGATATTTTTGGCGGCGGACGTGgtc                                                                                                                                                               <  2:21575/249‑1 (MQ=255)                                                                                             gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGt                                                                                                                                                       >  1:461687/1‑251 (MQ=255)                                                                                                              caccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAGTTAAAGAGAGCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGa                                                                                                                                      <  2:281181/251‑1 (MQ=255)                                                                                                               accCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCt                                                                                                                                        <  2:411804/248‑1 (MQ=255)                                                                                                               accCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCt                                                                                                                                        >  1:411804/1‑248 (MQ=255)                                                                                                                                               gAGGCGAAATTTAAAGAGAGAAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGCGTCAACGTGCGGCGCGCGGTGCTGATTTACTCTATAACATTGAGCTCACCCTCgaaga                                                                                                     <  2:192727/251‑1 (MQ=255)                                                                                                                                                 ggCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCgaaga                                                                                                     >  1:294751/1‑249 (MQ=255)                                                                                                                                                                             tATGCAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCCGCGATATTTTTGGTGCAGTTTTCGGCGGTATTTTTGGCGGCGGACGGGGTCGTCAACGTGCGGCGCGCGGGTCTGATTTACGCTATAACCTGGCGCTCACCCTCGAAGAAGCTGTACTGGGCGTGCCCACAGGGATCCg                                                                       >  2:103257/1‑251 (MQ=255)                                                                                                                                                                              aTGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCg                                                                       <  2:322697/250‑1 (MQ=255)                                                                                                                                                                              aTGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGc                                                                      >  1:440440/1‑251 (MQ=255)                                                                                                                                                                                   gTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTcc                                                                 <  1:118694/251‑1 (MQ=255)                                                                                                                                                                                         aCCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCCAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGGGGTCGTCAACGGGCGGCGCGCGGTGCTGATTTACGCTCTAACATGGAGCTCACCCTCGACAAAGCTGTACGTGGCGTGACCCAAGGGATCCGCAttacgccccc                                                           >  2:59129/1‑243 (MQ=255)                                                                                                                                                                                         aCCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGActct                                                           <  2:182923/251‑1 (MQ=255)                                                                                                                                                                                                 gCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGAGGTATGGGCGGCGGCGGTTTTGGCGGCGGAGCAGACTTCAGCGATATTTTTGGAGACGTTTTAGGCGCTATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGAGGTGGTGATTTACGCTATAACCTGGAGCTCACCCTCGACGAAGCTGTACGTGGCGTGACCAAAGCGATCCGCATTCCGACTCTggccgagg                                                   >  1:443558/1‑245 (MQ=255)                                                                                                                                                                                                   aaaaaCGTGCGGCATACGGTCAGTATGGTCAGGCTGCGGTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCTGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAgtgt                                                 <  2:462793/251‑1 (MQ=255)                                                                                                                                                                                                             ggCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGcc                                       >  1:166105/1‑251 (MQ=255)                                                                                                                                                                                                                   cGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTa                                 <  2:435620/251‑1 (MQ=255)                                                                                                                                                                                                                   cGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGCGTCAACGTGCGGCGCGCGGTGCTGGTTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGCCTCTGGAAGAGTGTGACGTTTGCCACGGt                                  >  2:260160/1‑250 (MQ=255)                                                                                                                                                                                                                    gATCAGTCTGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGCTTTTGGCGGCGGCGCCGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATCTTTTTGGAGGCGGACGGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTCTAACATGGATCTCCCCCCCGAAGAAGCTGTCCGTGGCGTGCCCACAGCGACCCGAATTCCGACTCTggcgaagggaggcgtttgccacggt                                  >  2:204580/1‑226 (MQ=255)                                                                                                                                                                                                                       cAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGCGCTCACACTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGCAGAGTGTGACGTTTGCCACGGTAGCgg                             >  1:56594/1‑251 (MQ=255)                                                                                                                                                                                                                        aGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGCCGTTTGCCACg                                    >  1:285211/1‑243 (MQ=255)                                                                                                                                                                                                                        aGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGAGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGGAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGCCTCTGGAAGAGGGTGACGTTTGCCACGGTAGCggg                            >  1:305460/1‑250 (MQ=255)                                                                                                                                                                                                                        aGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGGGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGGTCCGCATTCCGACTCTGGAAGAGGGTGACGTTTGCCACGGTAGCggg                            >  2:309172/1‑250 (MQ=255)                                                                                                                                                                                                                        aGTATGGTCATGCTGCGGTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCCGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGGCGTGGTCGTCAACGTGGGGCGCGCGGGGCTGATTTACGCTATAACATGGAGTTCACCCTCGAGGAAGCTGGACGTGGCGTGAACCAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGAAGTTTGCCACGGGAGCggg                            >  2:283488/1‑250 (MQ=255)                                                                                                                                                                                                                        aGTATGGTCATGCTGCGGTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTGTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACg                                    <  2:285211/243‑1 (MQ=255)                                                                                                                                                                                                                          tctGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGGTTTCGGCGATATTTTTGGCGGCGGGCGTGGTCGTCAACGTGCGGCGCGCGGGGCTGATTTCAGCTATAACCTGGAGATCACCCTCGAAGAAGTGGTAAGTGGGGGGACCCAAGAGATCCGCATGCCGACTCTGGTAGAGGGGGACGGTTGcgccggcagcgtggc                          >  2:372333/3‑237 (MQ=255)                                                                                                                                                                                                                                 aTGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGGCGTTTTCGGCGATCTTTTTGGCGGCGGGCGGGGTCGTCAACGTGCGGCGCGCGGTGCTGCTTTACGCTGTAACATGGAGCTAACCCTCCAAGAAGGTGTACGTGGCGTGACCCAAGGGATCCGCTTTCAGCCTCTGGAAGAGTGTGACGTTTGGCACGGTAGCGGGGCAAAAccc                   >  2:134947/1‑250 (MQ=255)                                                                                                                                                                                                                                   gctgcGTGTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTGCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAgg                 <  2:305460/251‑1 (MQ=255)                                                                                                                                                                                                                                        gTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACCTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGGCCAAAGAGATCCGCATTCCGACTCTGGGAGAGGGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTacac            >  2:199945/1‑251 (MQ=255)                                                                                                                                                                                                                                          ttGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGGGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATACGCATTCCGGCTCTGGAAGAGGGTGACGTTTGCCCCGGGAGCGGTGAAAAACCAGGGacac            >  2:12737/1‑249 (MQ=255)                                                                                                                                                                                                                                           tGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATCTTTTTGGGGGCGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAAAATGGAGCTCACCCTCGAAGAAGCTGGACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGGGGGACGGTGGCCACGGGAGCGGTGCAAAAACCGGTACACAgg         >  2:232665/1‑250 (MQ=255)                                                                                                                                                                                                                                                  ggtggtATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGGTATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGGTTTACGCCATAAACTGGAGCTCACCCTCGAAGAAGCTGTACGGGGCGTGACCAAAGAGGTCCGCCTTCCGCCTCTGGGAGGGGCGGACGTTTGCCACGGTGGCGGGGCAAAACCAGGAacacggcggacgac  >  2:149157/1‑241 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGAC  >  NC_000913/14155‑14646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑