Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	13,539	A→G	L459L (CTA→CTG)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	13,539	0	A	G	100.0%	182.8 / NA	54	L459L (CTA→CTG)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/24);  total (30/24)

CAGGGTGGTGTTCTGACTGGTGACGTAAAAGACGTACTGCTGCTGGACGTTACCCCGCTGTCTCTGGGTATCGAAACCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGA‑AATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGG  >  NC_000913/13294‑13778                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 cAGGGTGGTGGTCTGACTGGTGACGTGAAAGACGTACTGCTGCTGGACGTTACCCCGCTGTCTCTGGGTATCGAAACCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATgg                                                                                                                                                                                                                                             <  1:277184/251‑1 (MQ=255)    ggttgtgtttggACTGGTGACGTGAAAGCCGTACTGCTGCGGGACGTTACCCCGCTGTCTCTGGGTATCGAAACTATTGGCGGGGTGATGAGGACGCTGATCGCGAAAAGCACCACTAACCCGACCAAGCACAGCTAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGGAAACTTGCGGCTGATAACAAATCTCTGTGTCA‑GTTCAACCTGGAGGGTAt                                                                                                                                                                                                                                          <  2:434962/240‑1 (MQ=255)     gtggtgTTCTGACTGGTGACGTGAAAGACGTACTGCTGCTGGACGTTACCCCGCTGTCTCTGGGTATCGAAACCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATc                                                                                                                                                                                                                                         <  1:139841/251‑1 (MQ=255)                aCTGGTGACGTGAAAGACGTACTGCTGCTGGACGTTACCCCGCTGTCTCTGGGTATCGAAACCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGAAcc                                                                                                                                                                                                                              >  1:438885/1‑251 (MQ=255)                   ggTGACGTGAAAGACGTACTGCTGCTGCACGTTACCCCGATGTCTCTGGGCATCGAAACCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACcgcg                                                                                                                                                                                                                           >  1:126932/1‑251 (MQ=255)                            aaaGACGTACTGCTGCTGGACGTTACCCCGCTGTCTCTGGGTATCGAAACCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGcc                                                                                                                                                                                                                  >  1:129456/1‑251 (MQ=255)                                  gcaCTGCTGGTGGACGGTACCCCGCTGTCTCTGGGTATCGAAACCATGGGCGGTGGGATGACGACGCTGGTCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGAt                                                                                                                                                                                                            <  2:167887/249‑1 (MQ=255)                                  gTACTGCTGCTGGACGTTACCCCGCTGTCTCTGGGTATCGAAACCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCCCCGCGCGGCATGCCGc                                                                                                                                                                                                                >  2:297078/1‑247 (MQ=255)                                       gcttctggactgttccccGCTGTCTTTGGGGATCGAATCCATGGGCGGTGTGATGACGAAGCTGATCTCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTTAACGTAAGCGTGCGGCTGATAACAAACCTCTGGGTCA‑GTTCAACCTGGATGGAACCAACCCGGCACCGCGCGGCATTCCGCAgt                                                                                                                                                                                                             <  2:273104/231‑2 (MQ=255)                                            tgGACGTTACCCCGCTGTCTCTGGGTATCGAAACCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTAcc                                                                                                                                                                                                  >  2:380243/1‑251 (MQ=255)                                                cGTTACCCCGCTGTCTCTGGGTATCGAAACCATGGGCGGTGTGATGACGCCGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCg                                                                                                                                                                                              >  1:128976/1‑251 (MQ=255)                                                               tctGGGTATCGAAACCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAACCGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCCCCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACg                                                                                                                                                                               >  2:357476/1‑251 (MQ=255)                                                                       tCGAAACCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTg                                                                                                                                                                       >  2:145837/1‑251 (MQ=255)                                                                          aaaCCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACCCCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTTTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACTGTATCCTGCAc                                                                                                                                                                    <  2:283269/251‑1 (MQ=255)                                                                             cccggggccttgtgATGACGACGCTCATCGCGAAAAACACCTCTACCCCGACCAAGCACAGCCAGGGGTTCTCTGCCGCTGAAGACAACCAGTCTGCGGGAACCATCCATGTGCTGCAGGGTGAACTTAAACTTGCGGCTGATAACAAATCTCTGGGGCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTACCCTGCACGtt                                                                                                                                                                 <  2:250490/241‑1 (MQ=255)                                                                              cATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAACTCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGACGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGttt                                                                                                                                                                >  2:153787/1‑251 (MQ=255)                                                                                 gggCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTcc                                                                                                                                                              <  1:355001/250‑1 (MQ=255)                                                                                 gggCGGAGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCCCGCGGCATGCCGCAGATCGAAGTTACCTTCGTGATCGATGCTGACGGTATCCTGCACGTTTCcg                                                                                                                                                             >  2:43771/1‑251 (MQ=255)                                                                                      gggtgaggacgtcggcgATCGCGAAAAACACCACTATCCCGACCCAGCACAGCCAGTTGTTCGCTACCGGGGAAGACAAGCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGTTCA‑GTTCAAACTGGATGGTATCAACCCGGCTCCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGAGATCGATGCTGACGGTATCCTGCACGTTTCCGCGaaa                                                                                                                                                        <  2:289321/235‑1 (MQ=255)                                                                                        gtgATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGaa                                                                                                                                                         >  1:327652/1‑248 (MQ=255)                                                                                         tgatgaCGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGGATCCTGCACGGTTCCGCGaa                                                                                                                                                         >  1:399798/1‑247 (MQ=255)                                                                                          gatgaCGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGAt                                                                                                                                                     >  1:155746/1‑250 (MQ=255)                                                                                           atgaCGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCCGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGGCGGTATCCTGCACGTTTCCGCGAAAGATa                                                                                                                                                    >  1:161693/1‑250 (MQ=255)                                                                                               cgacgCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGTTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAAc                                                                                                                                               <  2:116303/251‑1 (MQ=255)                                                                                                gacgCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAAc                                                                                                                                               >  1:251603/1‑250 (MQ=255)                                                                                                                 cacccacTATCCCGACCAAACCCAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCACTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAATAGCagaag                                                                                                                             <  2:407190/248‑1 (MQ=255)                                                                                                                    ccacTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATc                                                                                                                          <  2:364523/251‑1 (MQ=255)                                                                                                                           cccGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATc                                                                                                                    >  2:164943/1‑250 (MQ=255)                                                                                                                              gACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAgg                                                                                                                >  1:459595/1‑251 (MQ=255)                                                                                                                              gACCAAGCACAGCCAGGTGTGCTCTACCGCTGAAGACAACCAGGCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAgg                                                                                                                <  2:128976/251‑1 (MQ=255)                                                                                                                                    gtacaGCCAGGTGTTCTCTCCCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGcttctt                                                                                                          <  2:126932/249‑1 (MQ=255)                                                                                                                                     cacaGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCttcttc                                                                                                         <  1:164943/251‑1 (MQ=255)                                                                                                                                      acaGCCAGGTGTTCTCTACCGCTTAAGACAACCAGTCGGCGGTAACCATCCATGTGCTGCATTGTGAACGTAAACGTGCGGCTGATAACAAAGATCTGGGTCA‑GTTCAACCTGGATGGTAGCAACCCTGCACCGCGCGGCATGCCGCATATCGAAGTTACCTTCGATATCGATGCTGACGTTATCCTGCACGTTGCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTtcttct                                                                                                        <  2:450109/251‑1 (MQ=255)                                                                                                                                        aGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTgg                                                                                                      >  1:233382/1‑251 (MQ=255)                                                                                                                                                gttttcgaCCGCGGAAGACAAACAGACTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGAGCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACTTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTtcttcttggttgaacg                                                                                              <  2:465807/244‑11 (MQ=255)                                                                                                                                                    tctacaggtGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGAGAACGTAAACGAGAGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGTCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCGGACGGTATCCTGCACGTATCCGAGGAAGATAAAAAAAGCGGTAACAAGAAGAAGATCAACATCACGGATTCTTCTGGACTGAACGa                                                                                             >  2:193256/9‑248 (MQ=255)                                                                                                                                                        ccGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGa‑a                                                                                     <  2:85442/251‑1 (MQ=255)                                                                                                                                                          gCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGACCGTAAACGTGCGGCTGATCACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGACGATCGAAGTTCCCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACAATCAAGGCTTCCTCTGGTCTGAAAGAAGATGa‑aa                                                                                    >  2:184635/1‑250 (MQ=255)                                                                                                                                                           cTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCa                                                                                                                                                                                                               >  2:424149/1‑127 (MQ=255)                                                                                                                                                           cTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAAGCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCa                                                                                                                                                                                                               <  1:424149/127‑1 (MQ=255)                                                                                                                                                            tGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCgg                                                                                                                                          >  2:138732/1‑195 (MQ=255)                                                                                                                                                            tGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCgg                                                                                                                                          <  1:138732/195‑1 (MQ=255)                                                                                                                                                              aaGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTGCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGA‑AATCCAg                                                                               <  1:297078/251‑1 (MQ=255)                                                                                                                                                                                tAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGCTAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGACCGAAGATGA‑AATCCAGAAAATGGTACGCAACGc                                                              >  2:11280/1‑250 (MQ=255)                                                                                                                                                                                    catccatGTGCTGCCGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGA‑AATCCAGAAAATGGTACGCGACGCAGa                                                           >  1:258532/1‑249 (MQ=255)                                                                                                                                                                                     atctatGTGCTGCAGGGTAAACGAAAACGTTCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATAAACCCGGCACAGCGCGGCATGCCGAAGATCGAAGTTACCTTCGATCTCGATGCTGACGGTATACGGCAAGTTTCCGCGCAAAAGAAAAACAGCGGTAAAGAGCAGGAGATCACCATCACGGAGTCTTCTTGTCTACACGAAGA‑GAGAATCAAGAAAATTGTACGCGaagaaac                                                           >  2:260044/5‑242 (MQ=255)                                                                                                                                                                                                                cGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGA‑AATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTg                              >  1:358217/1‑250 (MQ=255)                                                                                                                                                                                                                     ggCTGATAACAAATCTCTGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGA‑AATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGc                        >  1:307319/1‑251 (MQ=255)                                                                                                                                                                                                                         taTAACAAATATCTGG‑TCATGGTCAACCGGGAGGGTATCAACCCGGCACCTCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTTTCCTGCACGTTTCCGCGAAAGATAAAAACATCGGTAAAGAGCAGAAGATCACCATCAAGGCTGCTTCTGGTCTGAACGAAGATGA‑AAACCATAAAATGGTACGCGACTCATAAGCTACCGCCGAAGCTGACCGTAAGTTTGAAGAGCTgg                     <  2:339029/249‑1 (MQ=255)                                                                                                                                                                                                                          aTAACAAAGCTCTGGGGTA‑GTTCATCCCGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATTGAAGTTACCTTCGATATCGATGCTGACGTTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGA‑AATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGCCCGTAAGTTTGAAGAGCTGGTa                   <  2:330459/251‑1 (MQ=255)                                                                                                                                                                                                                                   ctctGGGTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCATGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGA‑AATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGACGAGCTAGTACAGACTCGc          >  1:32858/1‑251 (MQ=255)                                                                                                                                                                                                                                      tGGGTCA‑GTTGAACCTGGATGGTATCAACCCGGCAACGCGCGGCATGCCGCAGGTCGAAGTTACCTTCGATAGCGAGGCTGACGGTATCCTGCTCGTTGACGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGA‑AATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCAGAAGCTGACCGTAAGTGTGAAGAGCTGGTACAGACTCGCaa        <  2:99422/250‑1 (MQ=255)                                                                                                                                                                                                                                         gTCA‑GTTCAACCTGGATGGTATCAACCCGGCACCGAGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGCTACAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGA‑AATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCAGCCCGTAAGTTTGAAGATCTGGTACAGACTCGCAAcc      >  2:278790/1‑249 (MQ=255)                                                                                                                                                                                                                                           cA‑GTTCAACCTGGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGA‑AATCCAGAAAATGGTACGCGACGCAGAAGCTACCGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAggg  >  2:114853/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 CAGGGTGGTGTTCTGACTGGTGACGTAAAAGACGTACTGCTGCTGGACGTTACCCCGCTGTCTCTGGGTATCGAAACCATGGGCGGTGTGATGACGACGCTGATCGCGAAAAACACCACTATCCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCA‑GTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGA‑AATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGG  >  NC_000913/13294‑13778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑