Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,846	G→T	G228G (GGG→GGT)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,846	0	G	T	93.2%	159.2 / ‑1.2	62	G228G (GGG→GGT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (34/21);  total (40/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

AGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCC  >  NC_000913/12611‑13084                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               aGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGAc                                                                                                                                                                                                                                 >  1:323535/1‑251 (MQ=255)  gCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTAGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACa                                                                                                                                                                                                                                <  1:112496/251‑1 (MQ=255)   cGTCAGGCAACCCAAGACGCAGGCCGTATCGGTGGTCTGGAAGTAAAACGTATCAGCAACGAACCGACCGCAGCTGCGCTGGCTTCCGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAg                                                                                                                                                                                                                               <  2:168070/251‑1 (MQ=255)    gTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGTCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGCCGAAGTTGACGGCGAAAAAACCTTCGAAATTCTGGCAACCACCGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGc                                                                                                                                                                                                                              >  2:364972/1‑251 (MQ=255)          cAACCAAAGACGCAGGCAGTATCGCTGGTAAGGAAGTAAAACGTATAATCAACGAACAGACCGCAGCTGCGCTGGCTTACGGTATGGACAAAGGTACTGGCAACCGTACTAACGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGAGATACCCACATGGGAGGTGAAGACTTCGACAGCCGTc                                                                                                                                                                                                                          >  1:320775/1‑249 (MQ=255)           aaCCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGATCTGGCAACCAACGGTGATACCCACCTGGGTGGGGAAGACTTCGACAGCCGTCTg                                                                                                                                                                                                                        >  2:177327/1‑250 (MQ=255)                     ccAGGCCGTATCGCTGGTCGGGAAGTAAAACGTACCAGCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACATAGGTACTGGAAACCGTACTAGCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTTTATTATCGAAAGCGACTATGGCGACGGCGAAAAATCCTGCGATGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTacct                                                                                                                                                                                                             <  1:41453/250‑4 (MQ=255)                                     gTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGCCGGCGACAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGGGAAGACTTCGACAGACGTCTGATCAACTACCTGGTTGAAGAATTcga                                                                                                                                                                                              >  2:190677/1‑248 (MQ=255)                                         ggaatgaaaaCGTATCATCAACTAACCGACAGCATCTGCGCTTGCTTACGGGGTGGACAAAGGGCCTTGCATTCGTACTAGCGCTGTTTATGACCTGGGTGGTGGTACTTTCGATATCTCTATTATCGATAGCGCCGAAGTTTACGTTGAAAATACTGTCGAATTTCTGGCAATCAACGGTGATTACCTCCTGGGTGGTGAAGTCTTCGACAGCCGCCTGATCCACTGTCTGGTTGATGAACTCacgaaa                                                                                                                                                                                          <  2:316707/244‑1 (MQ=255)                                          gAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGAAGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGAGGTGAAGACTTCGACAGCCGTATGATCAACTACCTGGTTGAAGTATTCaaaaaaa                                                                                                                                                                                         >  2:386925/1‑249 (MQ=255)                                           aaGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGAGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCaagaa                                                                                                                                                                                           >  2:21352/1‑247 (MQ=255)                                                aaaCGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTAAAGAAAGATCAggg                                                                                                                                                                                  >  2:293155/1‑251 (MQ=255)                                                               gAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCa                                                                                                                                                                    >  1:3089/1‑250 (MQ=255)                                                                     accgCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTgaa                                                                                                                                                                                                      <  2:340835/210‑1 (MQ=255)                                                                     accgCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTgaa                                                                                                                                                                                                      >  1:340835/1‑210 (MQ=255)                                                                             tGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCg                                                                                                                                                                                                                                   <  1:447952/173‑1 (MQ=255)                                                                             tGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCg                                                                                                                                                                                                                                   >  2:447952/1‑173 (MQ=255)                                                                                gcTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGc                                                                                                                                                  >  1:54778/1‑251 (MQ=255)                                                                                 cTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCCACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGCCCTGCGCAACGATCCGCTGGCaaggc                                                                                                                                                  >  2:228947/1‑247 (MQ=255)                                                                                  tGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGc                                                                                                                                                  >  1:389273/1‑249 (MQ=255)                                                                                        aCGGTCTGGACAAAGGTACTGGCAACCGTACGAGCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACGTCCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGTTGGCAATGCAGCGCCt                                                                                                                                           <  2:119099/250‑1 (MQ=255)                                                                                               ggACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGTAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGGGAAGACTTCGACAGCCGTATGATCAACTACCTGGTTGAAGAATTCAAGAAGGATAAGGGCATTAACCTGCGCAACGTTCCGCTGGAAATGCAGAGCCTGAaaga                                                                                                                                     >  2:94724/1‑249 (MQ=255)                                                                                                            ggCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTGCTATTATCGAAATCGACTAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAAGTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaaaaa                                                                                                                        <  1:211800/249‑1 (MQ=255)                                                                                                             gCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCg                                                                                                                     >  2:80637/1‑251 (MQ=255)                                                                                                               aaCCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGa                                                                                                                    >  1:434962/1‑250 (MQ=255)                                                                                                                   gTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATc                                                                                                               <  1:122104/251‑1 (MQ=255)                                                                                                                    tACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTg                                                                                                                                                                                                        <  2:314174/161‑1 (MQ=255)                                                                                                                    tACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTg                                                                                                                                                                                                        >  1:314174/1‑161 (MQ=255)                                                                                                                        aTCGCGGTTTATGACCTGGGTGGTGGGTCTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACt                                                                                                          <  1:274601/251‑1 (MQ=255)                                                                                                                        aTCGCGGGTTATGACCTGGGTGGTGGTACTTTCGATATTGCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAAc                                                                                                           <  2:431102/250‑1 (MQ=255)                                                                                                                            cgGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCt                                                                                                      >  1:183874/1‑251 (MQ=255)                                                                                                                              gTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTc                                                                                                    >  1:174506/1‑251 (MQ=255)                                                                                                                                          ggtggtggtACTTTCGATATTTCTATTATCGAAAGCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTTGGTGGTGAAGACTGCGACAGCCGTCTGATCAACTACCGGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCGGTGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGAc                                                                                        <  1:439904/251‑1 (MQ=255)                                                                                                                                                ggtACTTTCGATATTTCTATTATCGAAATCGACGTAGTTGAAGGCGATAAAACCTTCGAAGTTCTGGCAACCAACGGCGATACCCACCTGGGTGGTGAAGACTTCGCCAGCCGTCTGATCAAGTCACTGGTCGAAGAATTCAAGAAAGATCAGAGCATTGAACTGCGCAACGCTCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAATGCTAAAATCGACCTGTCTTCCGCTCAGCAGACCGACGt                                                                                  >  1:76618/1‑251 (MQ=255)                                                                                                                                                  tACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGtt                                                                                 >  1:370497/1‑250 (MQ=255)                                                                                                                                                    cTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCGACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAAc                                                                              >  2:421139/1‑251 (MQ=255)                                                                                                                                                        cGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGc                                                                          >  2:1661/1‑251 (MQ=255)                                                                                                                                                          atatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCa                                                                                                                                                                                                                    >  2:30414/1‑111 (MQ=255)                                                                                                                                                          atatTTCTATTATCGAAACCGACGAAGTTGACGGCGAAAAATCCTTCGAAGTTCTGGCAACCAACGGGGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCa                                                                                                                                                                                                                    <  1:30414/111‑1 (MQ=255)                                                                                                                                                                 tattatCGAAATCGACGAAGTTGACGGCGAAGAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGACATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGGCTTCCGCTCAGCAGACCGGCGTTAACCtacagtacatcc                                                                 >  1:339029/1‑240 (MQ=255)                                                                                                                                                                  attatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACAt                                                                   <  1:110388/248‑1 (MQ=255)                                                                                                                                                                    tatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTg                                                              >  1:154582/1‑251 (MQ=255)                                                                                                                                                                        aaaaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCAGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCGGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGa                                                          <  2:323535/250‑1 (MQ=255)                                                                                                                                                                               acgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGAcc                                                   >  1:387404/1‑251 (MQ=255)                                                                                                                                                                                 gaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGCCGTTAACCTGCCATACATAACTGCAGACGCGACCgg                                                 >  2:147455/1‑251 (MQ=255)                                                                                                                                                                                 gaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGAGGTGAAGCCTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGCCGTTAACCGGCCGTACATCACTGCAGATGCGGCCgg                                                 >  2:139841/1‑251 (MQ=255)                                                                                                                                                                                             gAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGAtgcgcac                                                   >  2:306590/1‑234 (MQ=255)                                                                                                                                                                                             gAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGAcc                                                   <  1:306590/237‑1 (MQ=255)                                                                                                                                                                                                aaaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGaa                                  <  2:400117/251‑1 (MQ=255)                                                                                                                                                                                                   aCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACCCATGAACAt                               >  1:96435/1‑251 (MQ=255)                                                                                                                                                                                                      gtggAAGTTCGGGAAACCAACGGGGAGACCCACCAGGATGGTGAGGACTTCGTCAGCCGTCTGACAAACTACCTGGTGCAAGAAGTCAAGAAATAACAGGGCATGCACCTGGCCAACGTTCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGTAGAAAAAGTGAAAACCGAACGGTCTCCCGCTCAGCAGACGCACGTTAACCGGGCTTACATCACTGCAGATGCGACTGTACCGAAACACATGAACATCaa                            <  2:36182/248‑1 (MQ=255)                                                                                                                                                                                                               cgggCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCg                   <  2:370497/249‑1 (MQ=255)                                                                                                                                                                                                                tGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGt                  >  1:289321/1‑251 (MQ=255)                                                                                                                                                                                                                     accaacGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGCCCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGa              >  2:201929/1‑250 (MQ=255)                                                                                                                                                                                                                     accaacGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGa              >  1:199211/1‑250 (MQ=255)                                                                                                                                                                                                                         acGGTGATACCCTCCTGGGTGGTGAAGACTTCGACAGCCGGCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCGGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCTCTCAGCAGACTGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTg         <  1:314010/251‑1 (MQ=255)                                                                                                                                                                                                                           ggTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATAAACTACCTGCTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGCTGCGACCGGTCCGAAACACATGACCATCAAAGTGACTCGTGCGAAACTgg        >  2:390165/1‑250 (MQ=255)                                                                                                                                                                                                                             tGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAACGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGAGGCAGCAAAAGCGAAAATCGAACAGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGACCATCAAAGTGACTCGTACGAAACTGGaa      >  1:200910/1‑250 (MQ=255)                                                                                                                                                                                                                                tACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAACGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGCCCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAACGcc  >  2:222941/1‑251 (MQ=255)                                                                                                                                                                                                                                tACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCCGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAACAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACATGCCGTACATAACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTACGAAACTGGGAAGcc  >  1:450109/1‑251 (MQ=255)                                                                                                                                                                                                                                     aCCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTca                                                                                              <  1:191785/154‑1 (MQ=255)                                                                                                                                                                                                                                     aCCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTca                                                                                              >  2:191785/1‑154 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               AGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCC  >  NC_000913/12611‑13084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑