Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,714	C→T	G184G (GGC→GGT)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,714	0	C	T	90.7%	98.6 / 1.6	44	G184G (GGC→GGT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (23/16);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

GGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGA  >  NC_000913/12467‑12953                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 gggggtaaataaaaagaaaaaaaaTGACCCCGCCGCAGATCTCTTCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGTAGCTGTTATCACCGTACCGGCATACGTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTTTGGAAGTAAAACGTATCATCAACGAGCCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACt                                                                                                                                                                                                                                               <  2:176929/231‑1 (MQ=255)               ggctaaaaaaTGGCACCGCCGCAGATCTCTGCTTAAATGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTATCTGAAGCTGTTATAACCGTACCGGCATACTTTAACGATGCGCAGCGTCAGGCAACCACAGTCGCAGGCCGTATCGCTGGTCTGGAAGTATAACGTATCATCAACGAACCGACCGCAGCTGCGCTGATTTATGGTCTTGACAAAGGTACTTGCAACCGTACTAt                                                                                                                                                                                                                                 <  1:149017/245‑1 (MQ=255)                 acaaaaaaTGACCCCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATcg                                                                                                                                                                                                                               <  2:130462/247‑1 (MQ=255)                   aGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATcgcg                                                                                                                                                                                                                             <  1:90932/251‑1 (MQ=255)                             cccgccgCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGAcc                                                                                                                                                                                                                   <  2:112804/250‑1 (MQ=255)                               cgccgcAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCCAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGCCCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTg                                                                                                                                                                                                                 >  2:453743/1‑251 (MQ=255)                                gccgcAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCGACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTCTCGCGGTTTATGACCTgg                                                                                                                                                                                                                >  2:110388/1‑251 (MQ=255)                                        tctCTGCTGAAGTGCTGCAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAGCGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCGTACGGTCTGGACAAAGGTACTGGCAACCGTCCTATCGCGGTTTATGACCTGgggggtggt                                                                                                                                                                                                        >  2:390423/3‑251 (MQ=255)                                                gAAGTGCTGAAAAAAATGAAGAAAACCGCTGAATATTACCTGGGTGAACCGTTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCATCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGTCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCg                                                                                                                                                                                                 <  1:232594/250‑1 (MQ=255)                                                  aGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGat                                                                                                                                                                                               >  2:127972/1‑250 (MQ=255)                                                    tGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGATATCACCGTACCGGAAGACTTTAACGATGCTCAGCGTCAGGAAACCACAGACGCAGACCGTATCGCTGGTCTGGAAGTAAAACGTATCATAAACGAGCCGACCGCACCTGAGCTGGCTTAGGGTCTGGACATAGGTACTGGCAACCGCACTCTCGAGGATTATGACCTGGGGGGTGGTACTATCGATAtt                                                                                                                                                                                            >  1:36182/1‑251 (MQ=255)                                                     gCTGAAAAAAATGAAGAAAACCGCTGAAGAGTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATAtt                                                                                                                                                                                            <  1:127972/250‑1 (MQ=255)                                                         aaaaaaaTGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCtat                                                                                                                                                                                       >  1:407487/1‑251 (MQ=255)                                                                                                 cGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑CCGGCGAAAAAACCttaga                                                                                                                                               >  2:112496/1‑247 (MQ=255)                                                                                                    aaattgaggCTGTTACCACCGGACCGGCAGCCTGTAACGATGCTCAGCGTCAGGAAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTTTCATCAACGAACCGACCGCAGCTGCGCTTGCTTACGGTCTGGACAAAGGTACTGTCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTGTCGATATTTCTAGTATCGAAATCGACGAAGTTG‑ACGTCCAAAATACCTTCGAAGtt                                                                                                                                           <  2:235557/243‑1 (MQ=255)                                                                                                      aCTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCGGGGTGTGGGTACTTTCGATATTTTTATTATCGAATTCGACGAGATTA‑ACGGCGCAAAACCCTTTGAAGTTCt                                                                                                                                         >  1:322715/1‑251 (MQ=255)                                                                                                          aaGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGca                                                                                                                                     <  2:153041/251‑1 (MQ=255)                                                                                                                          aCCGGTATACTTTAACGCAGCTCCGCGTCAGGCAACAAAAGACGCAGGCCGTACCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCCGCGCTGGCTTACGGTCGGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTAGCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACAAACGGTGATcccc                                                                                                                     <  2:85784/251‑1 (MQ=255)                                                                                                                           ccGGCCTACTTTAACGATGCTCAGCGGCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCa                                                                                                                    <  2:403294/251‑1 (MQ=255)                                                                                                                                 caatttcaCGATGCTCAGATTCCGGCAACCAACGACGCAAGCCGCATCGCTGGTCTGGAAGTAAAACGTATAATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAACGGTAATGGCACCAGTACTATCGCGGTTTATGACCAGGGTGGTGGCAGTTTCGTAATTTCTATTATCGAAACCGACGA‑GTTGGAGGGCGAGAACACTTTAGAAGTTCTGGCCACAAACGGTGTTACCCACCTggg                                                                                                              >  2:21313/8‑251 (MQ=255)                                                                                                                                                 aGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGAc                                                                                              >  1:323535/1‑251 (MQ=255)                                                                                                                                                  gCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTAGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACa                                                                                             <  1:112496/251‑1 (MQ=255)                                                                                                                                                   cGTCAGGCAACCCAAGACGCAGGCCGTATCGGTGGTCTGGAAGTAAAACGTATCAGCAACGAACCGACCGCAGCTGCGCTGGCTTCCGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAg                                                                                            <  2:168070/251‑1 (MQ=255)                                                                                                                                                    gTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGTCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGCCGAAGTTG‑ACGGCGAAAAAACCTTCGAAATTCTGGCAACCACCGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGc                                                                                           >  2:364972/1‑251 (MQ=255)                                                                                                                                                          cAACCAAAGACGCAGGCAGTATCGCTGGTAAGGAAGTAAAACGTATAATCAACGAACAGACCGCAGCTGCGCTGGCTTACGGTATGGACAAAGGTACTGGCAACCGTACTAACGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGAGATACCCACATGGGAGGTGAAGACTTCGACAGCCGTc                                                                                       >  1:320775/1‑249 (MQ=255)                                                                                                                                                           aaCCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGATCTGGCAACCAACGGTGATACCCACCTGGGTGGGGAAGACTTCGACAGCCGTCTg                                                                                     >  2:177327/1‑250 (MQ=255)                                                                                                                                                                     ccAGGCCGTATCGCTGGTCGGGAAGTAAAACGTACCAGCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACATAGGTACTGGAAACCGTACTAGCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTTTATTATCGAAAGCGACTATGGCG‑ACGGCGAAAAATCCTGCGATGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTacct                                                                          <  1:41453/250‑4 (MQ=255)                                                                                                                                                                            gTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTc                                                                                                                                                 <  2:76380/173‑1 (MQ=255)                                                                                                                                                                            gTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTc                                                                                                                                                 >  1:76380/1‑173 (MQ=255)                                                                                                                                                                                     gTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑CCGGCGACAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGGGAAGACTTCGACAGACGTCTGATCAACTACCTGGTTGAAGAATTcga                                                           >  2:190677/1‑248 (MQ=255)                                                                                                                                                                                         ggaatgaaaaCGTATCATCAACTAACCGACAGCATCTGCGCTTGCTTACGGGGTGGACAAAGGGCCTTGCATTCGTACTAGCGCTGTTTATGACCTGGGTGGTGGTACTTTCGATATCTCTATTATCGATAGCGCCGAAGTTT‑ACGTTGAAAATACTGTCGAATTTCTGGCAATCAACGGTGATTACCTCCTGGGTGGTGAAGTCTTCGACAGCCGCCTGATCCACTGTCTGGTTGATGAACTCacgaaa                                                       <  2:316707/244‑1 (MQ=255)                                                                                                                                                                                          gAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑AAGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGAGGTGAAGACTTCGACAGCCGTATGATCAACTACCTGGTTGAAGTATTCaaaaaaa                                                      >  2:386925/1‑249 (MQ=255)                                                                                                                                                                                           aaGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGAGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCaagaa                                                        >  2:21352/1‑247 (MQ=255)                                                                                                                                                                                                aaaCGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTAAAGAAAGATCAggg                                               >  2:293155/1‑251 (MQ=255)                                                                                                                                                                                                               gAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCa                                 >  1:3089/1‑250 (MQ=255)                                                                                                                                                                                                                     accgCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTgaa                                                                   >  1:340835/1‑210 (MQ=255)                                                                                                                                                                                                                     accgCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTgaa                                                                   <  2:340835/210‑1 (MQ=255)                                                                                                                                                                                                                             tGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCg                                                                                                <  1:447952/173‑1 (MQ=255)                                                                                                                                                                                                                             tGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCg                                                                                                >  2:447952/1‑173 (MQ=255)                                                                                                                                                                                                                                gcTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGc               >  1:54778/1‑251 (MQ=255)                                                                                                                                                                                                                                 cTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCCACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGCCCTGCGCAACGATCCGCTGGCaaggc               >  2:228947/1‑247 (MQ=255)                                                                                                                                                                                                                                  tGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGc               >  1:389273/1‑249 (MQ=255)                                                                                                                                                                                                                                        aCGGTCTGGACAAAGGTACTGGCAACCGTACGAGCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACGTCCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGTTGGCAATGCAGCGCCt        <  2:119099/250‑1 (MQ=255)                                                                                                                                                                                                                                               ggACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGTAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGGGAAGACTTCGACAGCCGTATGATCAACTACCTGGTTGAAGAATTCAAGAAGGATAAGGGCATTAACCTGCGCAACGTTCCGCTGGAAATGCAGAGCCTGAaaga  >  2:94724/1‑249 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 GGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTG‑ACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGA  >  NC_000913/12467‑12953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑