Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,507	T→C	I115I (ATT→ATC)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,507	0	T	C	97.8%	142.2 / NA	46	I115I (ATT→ATC)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (23/22);  minor base A (1/0);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

TCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGG  >  NC_000913/12261‑12748                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  ccGCACCACGCTTTCAATCATTGCCTATACCCAGGAGGGTGAAACTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACCCCCTGTTTGGGATAAACCGCCTGATTGGCCGCCGCTCC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCAGGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTctg                                                                                                                                                                                                                                               <  1:76926/250‑1 (MQ=255)   gCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACAGCCGCAGATCTctgct                                                                                                                                                                                                                                             >  1:168070/1‑251 (MQ=255)                        ccTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGACCCCGCAAACCCCCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCGGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATgaag                                                                                                                                                                                                                        <  1:202140/251‑1 (MQ=255)                          tataCCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGGGCTGAAAAAAATgaaga                                                                                                                                                                                                                       >  2:30164/1‑250 (MQ=255)                              cccAGGATGTGGAAACTCTGGTGGGTCGGCCGGCTAACCGTCAGGCATTGACGAACCCGCAAAACCCCCTGTTTGCGATAAAACGCCTGATTGGCCGCCGTTCC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGTTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAAcc                                                                                                                                                                                                                  <  1:278785/251‑1 (MQ=255)                                   gATGGTGAAACTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTg                                                                                                                                                                                                              >  1:153041/1‑250 (MQ=255)                                      ggTGAAACTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGCAGTGCTGAAAAAAATGAAGAAAACCGCTGa                                                                                                                                                                                                             >  2:52885/1‑248 (MQ=255)                                       ctcccagtcggggtggccggCCGGCAAAACGGCAGGCAGGGACAACCCCGAAAACCACCCGGTTGGCGATTAAACGCCTGATTGGCCGCCGCTTC‑AAGGCCGAAAAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTAAAAATT‑ATTGCTGCTGATAACGGCGACGAATGGGTCGAAGTAAAAGGCAAAAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAg                                                                                                                                                                                                           <  2:477945/230‑1 (MQ=255)                                          aaaCTCTGGTTGGTCATCCGGCTAAACGTCACGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGAGATGTTT‑CC‑ATCATGCCGTTCAGAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATTGCCACGCCGCTGATCTCTGCTGAAGAGCTGAAAAAAATGAAGAAACACGCTTAAGATTa                                                                                                                                                                                                       >  1:376084/1‑250 (MQ=255)                                             ctctGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTg                                                                                                                                                                                                   >  1:112804/1‑251 (MQ=255)                                             ctctGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGACGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTg                                                                                                                                                                                                   >  1:268547/1‑251 (MQ=255)                                                 ggTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAg                                                                                                                                                                                                           >  1:477945/2‑239 (MQ=255)                                                    gggccAGCCGGCTAAACTTCAGGAAGTGACGAACCCGCAAAACCCCCTGTTTCCGATTAAACGCCTGATGGGCCGCCGCTTC‑CAGGACAAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCAGGGTTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAAc                                                                                                                                                                                            <  1:81940/247‑1 (MQ=255)                                                     ggTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAAcc                                                                                                                                                                                           >  1:431102/1‑251 (MQ=255)                                                                tAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGa                                                                                                                                                                                  >  2:128294/1‑249 (MQ=255)                                                                                   aaCCCGCAAAACACACTGTTTGCGATTAAACGCCTGATTGGCCGCCGATTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCt                                                                                                                                                                                                                                     >  2:442692/1‑179 (MQ=255)                                                                                                   gtgtggtgtatAAACGCCTGAGGGGCCGC‑GCTTCGCAAGACGAAGAGGTACAGGGTG‑TGTTTTCA‑AGCATGCCGTTAAAAATT‑TTTGATGGTGATAACGGCGCCGCAGGGGGCGAAGTAAAAGGCAAAAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGTTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCt                                                                                                                                             <  2:479909/241‑1 (MQ=255)                                                                                                         cGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGCAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGATCAGCGt                                                                                                                                       >  2:130149/1‑251 (MQ=255)                                                                                                         cGATTAAACGCCTGATTGGCCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCgg                                                                                                                                       >  1:119933/1‑250 (MQ=255)                                                                                                             aaaccgccggatgggccgccgcTCC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTAAAAATT‑ATTGCTGCGGATAACGGCGACGCAGGGGTCGAAGTAAAAGGCAAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAgg                                                                                                                                   <  1:52885/235‑1 (MQ=255)                                                                                                              aaactcctgatggcccgcgccTTC‑CAGGGCGAAAAATCACAGCGT‑AAGTTT‑CCCACCATGCCGT‑CAAAATTTATTTCTGCTGATAACGCCGTCGCAGGGGTCGAATTTAACGCCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAgg                                                                                                                                   <  1:55706/230‑1 (MQ=255)                                                                                                              aaaCCCCTTATTGGCCCCCGTTTC‑CAGGACGAAAAATTACGGCGTGTTGTTT‑CC‑ATCGTGCCTTTCAAAATT‑TGTGCTGCTGATAACGGCGACGCATGGGTCGAATTTAAAGGCCAAAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAgg                                                                                                                                   <  1:265860/250‑1 (MQ=255)                                                                                                                     tGATTGGCCGCCGCTTC‑CAGGACGAAGAAGCACAGAGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGATGCTGATAGCGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGCAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCaa                                                                                                                            >  2:439904/1‑250 (MQ=255)                                                                                                                            cccccccTTC‑CAGGCCGAAGAAGTACGGCGTTTTTTTC‑CC‑ATCATGCCGTTAAAAATT‑ATTGCTTCTGATACCGGCGACGCATGGGTCGAATTTAAAGCCCAAAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAg                                                                                                                    <  1:128294/245‑1 (MQ=255)                                                                                                                               ccgcTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGcc                                                                                                                 >  1:400117/1‑251 (MQ=255)                                                                                                                                   atC‑CAGGTCTAAGACGTACAGCGGGCTGTTT‑CC‑ATCATGACGTTCACAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACAGCCGCAGATATCTGCTGAAGAGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTTAACCGGTAAATGAAGCTGTTATAACCGTACCGGCATACTTTAACGCTGCTCAGCGTCAGGCAACCAAAGACCCATGCCGTAt                                                                                                             >  2:56583/2‑251 (MQ=255)                                                                                                                                          gACGAAGAAGTACAGTGTGATTGTC‑CC‑TTCATCCCTTTAAAAATT‑TTTGTTGCTGATACCGGCGCCGCGTGGGGCAAAGTAAAAGGCAAAAAAATGCCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTgg                                                                                                       <  2:329292/251‑1 (MQ=255)                                                                                                                                            agaagaaGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTAAAAATT‑ATTGCTGCTGATAACGGCGACGCAGGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTc                                                                                                     <  1:392225/250‑1 (MQ=255)                                                                                                                                                  agTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGCCGCATGGGTCGAAGTAAAAGGCCAGAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAg                                                                                               <  1:90835/251‑1 (MQ=255)                                                                                                                                                                ttt‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTatcatc                                                                                 >  1:83849/1‑251 (MQ=255)                                                                                                                                                                    cc‑ATCATGCCGTTCAAAATT‑ATTGTTGCTTATAACGGCGCCGCATGGTTCGAAGTTAAAGGCAAAAAAAGGTCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAAc                                                                              <  1:375235/251‑1 (MQ=255)                                                                                                                                                                         caggCCGTTAAAATTT‑ATTGTAGCTGAAAACGGCAACGTAGGGGTAGAAGTAAAAGCCCAAAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAac                                                                          <  1:331539/248‑1 (MQ=255)                                                                                                                                                                           tGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGGGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGGATCGCTGGTCTGGAAGTACAACGTATCATCAACGAaccg                                                                        >  2:122104/1‑251 (MQ=255)                                                                                                                                                                             cctgtaaaaaTT‑ATTGCGGCTGATAAGGGCGAGGAATGGGTGTAAGTAAAAGGCAAGAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAaccgac                                                                      <  2:343399/245‑1 (MQ=255)                                                                                                                                                                                     aaTT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCt                                                                                                         >  1:319310/1‑208 (MQ=255)                                                                                                                                                                                               tgctgATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCCACGAACCGACCGCAGCTGCGCTGGCtt                                                     >  2:90932/1‑251 (MQ=255)                                                                                                                                                                                                     tAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGACCCTGGCACCGCCGCAGATCTCTGATGAAGTGATGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGAGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTCTCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCCGCTGCGCAGGCATACGGTc                                               >  2:274601/1‑251 (MQ=255)                                                                                                                                                                                                                   gggggtaaataaaaagaaaaaaaaTGACCCCGCCGCAGATCTCTTCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGTAGCTGTTATCACCGTACCGGCATACGTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTTTGGAAGTAAAACGTATCATCAACGAGCCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACt                                 <  2:176929/231‑1 (MQ=255)                                                                                                                                                                                                                                 ggctaaaaaaTGGCACCGCCGCAGATCTCTGCTTAAATGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTATCTGAAGCTGTTATAACCGTACCGGCATACTTTAACGATGCGCAGCGTCAGGCAACCACAGTCGCAGGCCGTATCGCTGGTCTGGAAGTATAACGTATCATCAACGAACCGACCGCAGCTGCGCTGATTTATGGTCTTGACAAAGGTACTTGCAACCGTACTAt                   <  1:149017/245‑1 (MQ=255)                                                                                                                                                                                                                                   acaaaaaaTGACCCCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATcg                 <  2:130462/247‑1 (MQ=255)                                                                                                                                                                                                                                     aGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATcgcg               <  1:90932/251‑1 (MQ=255)                                                                                                                                                                                                                                             gCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTaa                                                                                                                                                    <  2:198418/110‑1 (MQ=255)                                                                                                                                                                                                                                             gCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTaa                                                                                                                                                    >  1:198418/1‑110 (MQ=255)                                                                                                                                                                                                                                               cccgccgCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGAcc     <  2:112804/250‑1 (MQ=255)                                                                                                                                                                                                                                                 cgccgcAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCCAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGCCCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTg   >  2:453743/1‑251 (MQ=255)                                                                                                                                                                                                                                                  gccgcAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCGACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTCTCGCGGTTTATGACCTgg  >  2:110388/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  TCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTC‑CAGGACGAAGAAGTACAGCGTGATGTTT‑CC‑ATCATGCCGTTCAAAATT‑ATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGG  >  NC_000913/12261‑12748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑