| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,407 | C→T | S357S (AGC→AGT) | thrA → | fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,407 | 0 | C | T | 96.4% | 157.5 / ‑2.3 | 58 | S357S (AGC→AGT) | thrA | fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (29/25); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01 | |||||||||||
CCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAAT > NC_000913/1170‑1652 | cccTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGTTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCg < 1:279987/251‑1 (MQ=255) tAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGCTGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCCTAGTTCCGTGGTGCTGATTACGCAATCATCTTCCGACTACAGTATCCGTTTCTGCGTTc > 1:186718/1‑242 (MQ=255) tAAAAATACCGGAAATCCACAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTc < 2:186718/242‑1 (MQ=255) aaaaaTACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCg > 1:376125/1‑250 (MQ=255) aaaTACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACt < 1:458866/251‑1 (MQ=255) ccAGGTACGCTACTTGGTGCCAGCTGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGGTCAGCGTTTCCTGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCTCGCGCCCGTATTTCCGTGGTGCTGATTACGCAAGCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGATTGTGTGCGAGCTGAACGGGCa < 2:35869/249‑1 (MQ=255) aGGTACGCTCCTTGGTGCCAGCCGTGATGATGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATGACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc < 2:432366/251‑1 (MQ=255) ggTAAGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGCAAGGGTTGGTTGGCATGGCGGAGCGCGTGTTTTCAGCGGTGGCAGGCGCCCGTATTTCCGTGGAGCTGATTACGACATCACCTCCCGAATACAGATTCAGTTTCTGCCTTCCTCAAAGGGACTGGGTGCGCGCTGCACGGGCCATGcc > 2:351390/1‑250 (MQ=255) ccGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCGGACCCGGGGATGACAGGGATGGTTGGCATGGCGGCGCGCGTGGTTGCAGCGATGTCAGGCGCCCGGATTTCGGTGGTGGTGCATCCGCAATAATCTTCCGAATACAGTTTCAGGTTCTGCGGTCCGCAAAACGCCTGTGTGCGGGCTGTACGGGCAAAGAAGGCAGGGTTCCACCTGGaa > 1:193211/1‑250 (MQ=255) gaAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAaaga < 1:233208/251‑1 (MQ=255) gAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCTGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGGTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCtt < 2:352514/251‑1 (MQ=255) gAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCtt > 1:151720/1‑251 (MQ=255) ggTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGGGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGCCTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGGAGGCTTACTGGAGc > 2:49764/1‑251 (MQ=255) aGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTg < 1:189771/251‑1 (MQ=255) cATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGGGGTGCTGTTTACGCAATCATCTTCCGAATACAGTATCAGTTTCCGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGGGCCGCTGGcgg > 2:405549/1‑249 (MQ=255) aTTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTAACGCGCCCGTATTTCCGTGGTGCTGTTTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAGCTGCAAGACGGCTTACTGGAGCCGCTGGcggg > 2:121379/1‑248 (MQ=255) cAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAACGACTGTGTGCGAGCTGCACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTTGAGCCTCTGGcggagtcgg > 1:356379/1‑243 (MQ=255) cggAATAAGATGGCAATGGTCAGCGTTTCTGACCCGGGGATGAAAGGGATGGGTGGCATGGCGGCGCGCGTGTATGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAAACAGCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAGTGCAGGAAGAGATCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGAGGTGACGTAACg < 2:46057/249‑1 (MQ=255) gAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACggg > 1:231771/1‑250 (MQ=255) aaTAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATG‑TCGGCATGGCGGCGCGCGTCTTTGCAGCGCTGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACCGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCCGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTAGTGGAGCCGCTGGCAGGGACGGAACGGCTg > 2:144553/1‑251 (MQ=255) taataTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGCAGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGAGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGAGACGGAACGGATgg > 1:216803/5‑251 (MQ=255) taataTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGCGGAACGGGTgg > 1:471764/5‑251 (MQ=255) aaagaTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTgg < 2:78371/247‑1 (MQ=255) ataTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGGATTTCCGGGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGCAAGAAGGCTTACTGGGGCCGCTGGCGGTgcagggaagggtgggc > 2:224728/3‑236 (MQ=255) tGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGCCTGTGTGCGAGCTGAACGGGCAATGCAGGGAGAGTTCTACCTGGAACTGAAAGAAGGCCTACTGGAGCCTCTGGCGGTggagggacaggcgggcact > 2:321932/1‑233 (MQ=255) tGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGcgggggcagatcggaagaggccc > 1:228405/1‑229 (MQ=255) tGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAc < 2:228405/235‑1 (MQ=255) ggCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGcccaaaggt < 1:126821/250‑9 (MQ=255) aTGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGGTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTAtctc < 2:231771/251‑1 (MQ=255) cGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCCGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCCTCTTCCGAATACAGTATCAGTTTCTGCGGTCCGCAAAGCGACTGTGTGAGAGCTGAACGGGCAATGCAGGCAGAGTTCTACCTGGAACTGGAAGAAGGCTTTCTGGGGCCGATGGCGGTGAGGGGACGGCTGGGCAttttcccgggggtgg > 2:482138/1‑238 (MQ=255) gTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGGACGGCTGGCCATTATCTCGGTGGGAgg > 1:92439/1‑251 (MQ=255) gTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGGGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGGGACGGAACGGGTGGCCCTTTTCTCggggggggg > 1:246939/1‑244 (MQ=255) ccggcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGttt < 1:145823/113‑1 (MQ=255) ccggcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGttt > 2:145823/6‑118 (MQ=255) cccGGGGATGAAAGGGATGGTTGGCATGGCGGCGAGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGGGATggg > 1:395633/2‑249 (MQ=255) ccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTAt < 1:12345/251‑1 (MQ=255) gggATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTAACAATTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCCGGAAGAGTTCTACCTGGAACTGAAAGAGGGCATACTGGAGCCGCTGGCGGGGACGGAGAAGCTGGCCCTTATCTCgtggaggggggagggtatgtc > 2:398521/1‑231 (MQ=255) aaaGGGATGGGTGGCAGGGGGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCt‑t < 2:457735/251‑1 (MQ=255) tggCATGGTGGCGGGCGTGTTGGCCGCGATGTCACGCGCCCGTAGTTCCGTGGTGCTGATTACGCAATCAGCTTCCAAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGATTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGTTTACGGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGAtc < 2:25293/249‑1 (MQ=255) gCATGGCGTCGCGCGTGTTTGCAGCGATGTCCCGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCg < 1:357808/251‑1 (MQ=255) gCAGCGATGTCACGCGCCCGTATTGCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGcgct < 1:177271/251‑4 (MQ=255) cgcCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCCGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAata < 1:405549/251‑1 (MQ=255) gcCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGGGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAatat > 1:375113/1‑251 (MQ=255) gTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCGCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAAc < 2:92439/251‑1 (MQ=255) aTTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACa < 1:121379/250‑1 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATCCAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGGGGTAGGGGGTGGGATGCGCACCT‑TGCGAGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCCATATCAACAtt > 2:61588/1‑251 (MQ=255) cTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCCTTATCTCGGTGGGAGGGGATGGTAATAGCAACTGTG‑GTGGGATATCGGCGCAATACTTTTACGCCGTGGCCCGCGCCCATATCCACATTGTTGccctagt > 2:21150/1‑246 (MQ=255) cTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGGGGCAATATCAACATTTTCGCCATTGc > 1:81001/1‑251 (MQ=255) gATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTAGTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGc < 1:144553/249‑1 (MQ=255) gtttcGCAATCATCTTTCGTATACTGGATCAGTTTCGGCGTTCCGCATAGCGATGGGGTGCGAGCTAAACGGGCAATGCAGGAAGATTTCGACGTGGAAGTGACAGAAGGCTGACTGGAGGCGTAGGCGGTGACGGAACGGCTGGCCATGATCGCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAGTATCAACATTGTCGCCATTGCTCa < 2:239051/247‑1 (MQ=255) gCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGAt < 1:49764/251‑1 (MQ=255) atcatcTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGAtctt > 1:146347/1‑251 (MQ=255) tcatcTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTTCTCAGGGGtcttc > 1:481598/1‑251 (MQ=255) ttCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAgggg > 1:428648/1‑150 (MQ=255) ttCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTg < 2:428648/152‑1 (MQ=255) ccGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGGACGGCTGGCCATTATCTCGGTGGTAGGCGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTTCCGCGCTGGCCCGCGCCAATATCAAAATTGTCGCCATTGCTCAGGGATCTTCTGaaagc > 1:215168/1‑248 (MQ=255) aTACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGa < 1:333147/147‑1 (MQ=255) tACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTTAACGGGCAATGCAGGCAGAGTTCTACCTGGAACTGAAAGAATGCTTACTGGAGCCCCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGCGGCAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCTCCAATATCAACATTGCCGCCATTGCTCAGGGATCTTCTGAACGCTCAAt > 1:143812/1‑251 (MQ=255) | CCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCT‑TGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAAT > NC_000913/1170‑1652 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |