Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,854	A→G	intergenic (‑68/‑309)	yaaI ← / → dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,854	0	A	G	100.0%	130.8 / NA	40	intergenic (‑68/‑309)	yaaI/dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/24);  total (16/24)

GTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGATTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCA  >  NC_000913/11607‑12097                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    gTCGGCAGAGAGTTGGATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTc                                                                                                                                                                                                                                                  <  2:209251/251‑1 (MQ=255)               gTATGCGTTTCACAATGCGACAAACAGGAAGGTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAAtct                                                                                                                                                                                                                                     <  2:174731/250‑1 (MQ=255)                     gTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGCCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGGGGTAGAGGGATGACTACCCACTAGTTTACTGCTGTTAAAGAGATGGTTCAGGTACGTAAtcttttcttt                                                                                                                                                                                                                              >  2:241864/1‑251 (MQ=255)                     gTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTAAGGAAAGTAAtcttttcttt                                                                                                                                                                                                                              >  2:286625/1‑251 (MQ=255)                        tCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCttttt                                                                                                                                                                                                                            <  1:186508/250‑1 (MQ=255)                         cacaATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATAAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTCGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTttat                                                                                                                                                                                                                          >  1:46551/1‑251 (MQ=255)                                         ggAAGTTTCAGGGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGTCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTTCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTtgatga                                                                                                                                                                                                          <  2:304975/251‑1 (MQ=255)                                          gAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTtgatga                                                                                                                                                                                                          >  1:384214/1‑250 (MQ=255)                                                      ccAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGc                                                                                                                                                                                             <  1:460336/251‑1 (MQ=255)                                                         gATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGat                                                                                                                                                                                          >  1:44704/1‑251 (MQ=255)                                                                   gtCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCttt                                                                                                                                                                                 <  1:268553/249‑1 (MQ=255)                                                                        tACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCttt                                                                                                                                                                                 >  2:348390/1‑245 (MQ=255)                                                                        tACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCttt                                                                                                                                                                                 <  1:348390/245‑1 (MQ=255)                                                                                      atgacgcCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTc                                                                                                                                                             <  1:332934/247‑1 (MQ=255)                                                                                         acgcCGAGGAGTTTATGGGCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAAt                                                                                                                                                          <  2:165626/250‑1 (MQ=255)                                                                                                 gagTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAAGCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTc                                                                                                                                                  <  2:63708/251‑1 (MQ=255)                                                                                                      tATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGGTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGt                                                                                                                                              <  2:384214/250‑1 (MQ=255)                                                                                                            cGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGcc                                                                                                                                        <  2:373352/250‑1 (MQ=255)                                                                                                               ttGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCatat                                                                                                                                    >  1:163668/1‑251 (MQ=255)                                                                                                                  ccTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTtg                                                                                                                                                                                                              >  2:81822/1‑174 (MQ=255)                                                                                                                  ccTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTtg                                                                                                                                                                                                              <  1:81822/174‑1 (MQ=255)                                                                                                                     gcgcCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATcgcg                                                                                                                                <  2:44704/249‑1 (MQ=255)                                                                                                                     gcgcCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATcgcg                                                                                                                                <  1:241864/249‑1 (MQ=255)                                                                                                                             cagcacagcaTCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTgc                                                                                                                      <  1:286625/251‑1 (MQ=255)                                                                                                                                          ggCTATTCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGATTGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACaaa                                                                                                         <  2:402309/251‑1 (MQ=255)                                                                                                                                           gttaagcGCCAGGCTGGGGAAAAACGTAAAAACGGATTTCATATGGATTCGCTTAGTGTGAAGAGGTAGAGGGTTGATTACCCATTAGTTTATTGTTGATAAAGAGAAGGTTCAGGTACGTAACCTTTTCTTTGTATTACAATTTTGTGATGAATGCCTTGGCTGTGATTCATTCTTTATCTGAATAAAATTGCTGCCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACaaaa                                                                                                        <  2:275931/245‑1 (MQ=255)                                                                                                                                            cTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCttttt                                                                                                                                                                                                                            <  1:389755/134‑1 (MQ=255)                                                                                                                                                          ggCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTcc                                                                                         <  1:165601/251‑1 (MQ=255)                                                                                                                                                                                 tCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGAc                                                                  >  1:35036/1‑251 (MQ=255)                                                                                                                                                                                         aTTCTCTTAGTGGGAAGAGGTATAGGGATGAATACCCCCTAGTTTACTGCTGAAAAAGAAAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATGTTTTGATGAATGCCTTTGCTGCAATTCATTCTTTATCTGAAAAAAATTGCTGTCAATTTTACGTCTTTTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTGTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTta                                                          <  2:213035/251‑1 (MQ=255)                                                                                                                                                                                             tctTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTAcaca                                                                  >  2:142603/1‑236 (MQ=255)                                                                                                                                                                                             tctTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGAc                                                                  <  1:142603/239‑1 (MQ=255)                                                                                                                                                                                                        agagGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCagt                                           >  1:176929/1‑251 (MQ=255)                                                                                                                                                                                                               gagggATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTTTTTTTATTACAATTTTTGGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGtgagtgag                                    <  2:163668/249‑1 (MQ=255)                                                                                                                                                                                                                               cTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGa                     >  1:425877/1‑250 (MQ=255)                                                                                                                                                                                                                                tAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGaa                    >  1:235557/1‑250 (MQ=255)                                                                                                                                                                                                                                  gTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAAtt                 >  1:470473/1‑251 (MQ=255)                                                                                                                                                                                                                                     tACTGATGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTggg              >  1:380257/1‑251 (MQ=255)                                                                                                                                                                                                                                                 agagAAGGTTCATGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCAATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGtagatcgga  <  1:278014/251‑9 (MQ=255)                                                                                                                                                                                                                                                  gagaAGGTTCAGGTACGTAATCTTTTCTTTTTATTACCATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATACAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAAcc   >  2:160091/1‑249 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    GTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGATTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCA  >  NC_000913/11607‑12097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑