Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,541	G→A	S82S (AGC→AGT)	yaaI ←	DUF2541 domain‑containing protein YaaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,541	0	G	A	96.9%	89.1 / ‑4.0	32	S82S (AGC→AGT)	yaaI	DUF2541 domain‑containing protein YaaI
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (19/12);  total (20/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

CTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATT  >  NC_000913/11304‑11782                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  cTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTCTATTATGGCGTTAGTCGTCGCCGTTAATTTTCCGCGTG‑GCCATATCCGATGAGTTCACCGTCTGACCGGAAAAGGTGATTTTTGAGACGAAGCGTTTATTGTCGTTATCCCTGTTACTGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTCTTTCTGAAGGATTATTCAGACTCTGACTGgcgc                                                                                                                                                                                                                                      >  2:482718/1‑251 (MQ=255)            aagaaAAGCCAGATCTGAATCATGCAGGTAATTAACATTCATTTTTTGTGGTTGCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGGG‑GCCATATCCGATGAGTTCACCGTATGAACGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCt                                                                                                                                                                                                                           <  2:161444/251‑1 (MQ=255)             agaaAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCtt                                                                                                                                                                                                                          >  1:174731/1‑251 (MQ=255)                 aaTCCAGATCTGAATCATTCAGGTACTTAAATTTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCTTCGCCGATAAGTTTCAGCGTG‑GCCATAGCCGATGAGTTCACCGTATGACCGTAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTTATCCAGGCAGTGGTTTGCCCTTCTTTTAGTTCGGAAGGAATATGCAGACTCTGACTGGCGCTACGGGCGGCTTTGaa                                                                                                                                                                                                                      <  1:398725/251‑1 (MQ=255)                 aaTCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGTCGGCTTTGa                                                                                                                                                                                                                       >  1:169598/1‑250 (MQ=255)                             aaTCATTCAGGGAATTAACATTCATTTTTTGTGGTTTCTATATTCTTGCGTTAGTCGTCGCCGATAATTTTCAGC‑TGTGCCATATCCGATTAGTTCACCGTATGACCGGAAAATGTGATTTTTGAGACGCAGCGTTTATTTTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGTGCGGCTTTGAAATAAACCGATGc                                                                                                                                                                                                          <  1:454853/251‑1 (MQ=255)                                             aaCATTCATTTTTTGTGGTTTATATATTCTGGCGTTCGTCGTAGCCGATACTTTTCAGCGTG‑GCCATATCCGATGAGTTAACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCATTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAGCCGATGCACTGCTTAACTgcaa                                                                                                                                                                                           >  1:389602/1‑247 (MQ=255)                                                    attTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCAt                                                                                                                                                                                   >  1:402309/1‑251 (MQ=255)                                                             ggtttCTATATTCTGGCGTTAGTCGTCTCCGATAATTTTCAGCGTG‑GCCATATCCGATTAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGTTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCa                                                                                                                                                                           <  1:467771/247‑1 (MQ=255)                                                                    atatTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTCTAAATCGCCATGGTCGGCAGAGAGTTg                                                                                                                                                                   >  2:332934/1‑251 (MQ=255)                                                                                        gCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGAc                                                                                                                                               <  2:133913/251‑1 (MQ=255)                                                                                          cGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACa                                                                                                                                              >  2:165601/1‑250 (MQ=255)                                                                                               aTTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAgg                                                                                                                                        >  1:213035/1‑251 (MQ=255)                                                                                                          g‑gCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAgc                                                                                                                              <  1:377716/250‑1 (MQ=255)                                                                                                                              aCCGTATGACCGGACAAGGCGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATACAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGCCTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGATTAACTGTAAATCGCCATGGACGGCAGAGAGTTGTATTCGTTTCACAATGCGACAAACAGGACGTCTAAGCGCCAGATCGTTGGTTTCGtt                                                                                                          >  2:471351/1‑251 (MQ=255)                                                                                                                                   aTGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTAcgcg                                                                                                     >  1:274365/1‑251 (MQ=255)                                                                                                                                                   aTTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGcc                                                                                     >  1:209251/1‑251 (MQ=255)                                                                                                                                                                 gggTTTATTGTTGTTATCACTGTTAATGTTTATCCGGTCAGGGGGTTGTCCCTCTTTTATTTTTGAAGTAATATTCAGATTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGATGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTGTATgtt                                                                       <  2:371293/249‑3 (MQ=255)                                                                                                                                                                           tCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTg                                                             >  1:373352/1‑251 (MQ=255)                                                                                                                                                                                 tcacTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGt                                                       >  1:304975/4‑251 (MQ=255)                                                                                                                                                                                   acTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTGGCCTGCGCCGTgc                                                     >  1:341224/2‑251 (MQ=255)                                                                                                                                                                                      gTTAATGTTGATCCAGTCAGTGTTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTgcag                                                   <  2:274365/250‑1 (MQ=255)                                                                                                                                                                                           tGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGcagcacagc                                             >  1:258319/1‑251 (MQ=255)                                                                                                                                                                                              tGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCAt                                           <  1:482718/250‑1 (MQ=255)                                                                                                                                                                                              tGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATc                                          >  1:63708/1‑251 (MQ=255)                                                                                                                                                                                                   cagtcagtGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCt                                     >  1:356455/1‑251 (MQ=255)                                                                                                                                                                                                    agtcagtGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATg                                                                                                                                                   >  2:292403/1‑140 (MQ=255)                                                                                                                                                                                                    agtcagtGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATg                                                                                                                                                   <  1:292403/140‑1 (MQ=255)                                                                                                                                                                                                         gtGGTTTGTCCTTCTTTTATTTCTGAAGGAATATTCAGACGCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTTACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTTATCGc                               <  2:280523/251‑1 (MQ=255)                                                                                                                                                                                                              ttGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGTACAGCATCAGGCTAATCGCCAGGc                          >  1:257123/1‑251 (MQ=255)                                                                                                                                                                                                                        gttattaCTGAAGGAAGAATCAGACTCTGACTGGCGCTAAGGGTGGCTTTGAAATAAACGTATTGACCGCTTATCTGTAAAACGCCATGGGTGGCAGAGATTTGTATGTGTTTCCCAATGAGACAAGCAGGAAGTGTCATCGGCAGATCGTTGGTTTCGTTACGCGGCATGGCAATTACGCCGAGGAGGTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTATTCGCTAAGCTGGCGGa                   <  2:472092/247‑1 (MQ=255)                                                                                                                                                                                                                                      aaTATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATAGTAAAAAAGGAtt  >  1:59644/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  CTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATT  >  NC_000913/11304‑11782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑