Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,481	G→A	S102S (AGC→AGT)	yaaI ←	DUF2541 domain‑containing protein YaaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,481	0	G	A	95.7%	63.1 / ‑6.4	23	S102S (AGC→AGT)	yaaI	DUF2541 domain‑containing protein YaaI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (13/9);  minor base C (1/0);  total (14/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTG  >  NC_000913/11234‑11723                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   gCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTg                                                                                                                                                                                                                                                 <  1:353144/251‑1 (MQ=255)                   gCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCcagtca                                                                                                                                                                                                                               <  1:479964/251‑1 (MQ=255)                                tGGGTGAGCAATCGGGCGAAATTTGCCAACTGATCCTCACTACAATGCTGCAGAAAATCCAGATCTGAGTCCTTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCctt                                                                                                                                                                                                                  >  2:186508/1‑251 (MQ=255)                                                            aCTGTTCCTCACTACAATGCTGAAGAAACTCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGAGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAgact                                                                                                                                                                                      >  1:349491/1‑248 (MQ=255)                                                                       cTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTCTATTATGGCGTTAGTCGTCGCCGTTAATTTTCCGCGTG‑GCCATATCCGATGAGTTCACCGTCTGACCGGAAAAGGTGATTTTTGAGACGAAGCGTTTATTGTCGTTATCCCTGTTACTGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTCTTTCTGAAGGATTATTCAGACTCTGACTGgcgc                                                                                                                                                                           >  2:482718/1‑251 (MQ=255)                                                                                  aagaaAAGCCAGATCTGAATCATGCAGGTAATTAACATTCATTTTTTGTGGTTGCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGGG‑GCCATATCCGATGAGTTCACCGTATGAACGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCt                                                                                                                                                                <  2:161444/251‑1 (MQ=255)                                                                                   agaaAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCtt                                                                                                                                                               >  1:174731/1‑251 (MQ=255)                                                                                       aaTCCAGATCTGAATCATTCAGGTACTTAAATTTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCTTCGCCGATAAGTTTCAGCGTG‑GCCATAGCCGATGAGTTCACCGTATGACCGTAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTTATCCAGGCAGTGGTTTGCCCTTCTTTTAGTTCGGAAGGAATATGCAGACTCTGACTGGCGCTACGGGCGGCTTTGaa                                                                                                                                                           <  1:398725/251‑1 (MQ=255)                                                                                       aaTCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGTCGGCTTTGa                                                                                                                                                            >  1:169598/1‑250 (MQ=255)                                                                                                   aaTCATTCAGGGAATTAACATTCATTTTTTGTGGTTTCTATATTCTTGCGTTAGTCGTCGCCGATAATTTTCAGC‑TGTGCCATATCCGATTAGTTCACCGTATGACCGGAAAATGTGATTTTTGAGACGCAGCGTTTATTTTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGTGCGGCTTTGAAATAAACCGATGc                                                                                                                                               <  1:454853/251‑1 (MQ=255)                                                                                                                   aaCATTCATTTTTTGTGGTTTATATATTCTGGCGTTCGTCGTAGCCGATACTTTTCAGCGTG‑GCCATATCCGATGAGTTAACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCATTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAGCCGATGCACTGCTTAACTgcaa                                                                                                                                >  1:389602/1‑247 (MQ=255)                                                                                                                          attTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCAt                                                                                                                        >  1:402309/1‑251 (MQ=255)                                                                                                                                   ggtttCTATATTCTGGCGTTAGTCGTCTCCGATAATTTTCAGCGTG‑GCCATATCCGATTAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGTTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCa                                                                                                                <  1:467771/247‑1 (MQ=255)                                                                                                                                          atatTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTCTAAATCGCCATGGTCGGCAGAGAGTTg                                                                                                        >  2:332934/1‑251 (MQ=255)                                                                                                                                                              gCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGAc                                                                                    <  2:133913/251‑1 (MQ=255)                                                                                                                                                                cGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACa                                                                                   >  2:165601/1‑250 (MQ=255)                                                                                                                                                                     aTTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAgg                                                                             >  1:213035/1‑251 (MQ=255)                                                                                                                                                                                g‑gCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAgc                                                                   <  1:377716/250‑1 (MQ=255)                                                                                                                                                                                                    aCCGTATGACCGGACAAGGCGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATACAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGCCTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGATTAACTGTAAATCGCCATGGACGGCAGAGAGTTGTATTCGTTTCACAATGCGACAAACAGGACGTCTAAGCGCCAGATCGTTGGTTTCGtt                                               >  2:471351/1‑251 (MQ=255)                                                                                                                                                                                                         aTGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTAcgcg                                          >  1:274365/1‑251 (MQ=255)                                                                                                                                                                                                                         aTTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGcc                          >  1:209251/1‑251 (MQ=255)                                                                                                                                                                                                                                       gggTTTATTGTTGTTATCACTGTTAATGTTTATCCGGTCAGGGGGTTGTCCCTCTTTTATTTTTGAAGTAATATTCAGATTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGATGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTGTATgtt            <  2:371293/249‑3 (MQ=255)                                                                                                                                                                                                                                                 tCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTg  >  1:373352/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   GCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTG‑GCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTG  >  NC_000913/11234‑11723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑