Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,985	A→G	N124N (AAT→AAC)  V52V (GTA→GTG)	yaaW ← mbiA →	putative enzyme‑specific chaperone YaaW uncharacterized protein MbiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,985	0	A	G	98.0%	151.9 / ‑4.5	49	N124N (AAT→AAC)  V52V (GTA→GTG)	yaaW mbiA	putative enzyme‑specific chaperone YaaW uncharacterized protein MbiA
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (26/22);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

TTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGC  >  NC_000913/10744‑11225                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  ttaatgctgcgcccaCAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCTTCGTCCATATTCTTCCAGGTGTTACGc                                                                                                                                                                                                                                             >  2:411883/15‑248 (MQ=255)    atgctgcgcccaCAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCGTCGTCCATATTCTTCCAGGTGTTACGCAGAAAt                                                                                                                                                                                                                                       <  1:411883/240‑1 (MQ=255)        tgcgcccaCAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAACTTGtt                                                                                                                                                                                                                                   >  2:140460/8‑251 (MQ=255)          cgcccaCAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTcc                                                                                                                                                                                                                                 >  1:384801/6‑251 (MQ=255)           gcccaCAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCa                                                                                                                                                                                                                                <  1:254425/247‑1 (MQ=255)                       tCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCt                                                                                                                                                                                                                    >  2:339897/1‑251 (MQ=255)                        cccAGACCCGCACCTCCCAGCAAACAATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAACGCAAATACGACACGCCTATTGCCCATAATTTGTATTTCATCAGCAGCGGCAGCCGCTCTTCCAGCTAATTCACCCTGGCGTCGACCGCGTGCAGACCATCCTGCTTTTGTTCCTCGTCCCAATACTTCCAGGTGTTACGCAGAAATTGTTCAAGTAACTATTgttc                                                                                                                                                                                                                   >  2:398725/1‑248 (MQ=255)                                               aCCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCtt                                                                                                                                                                                            >  1:467312/1‑251 (MQ=255)                                                     cccAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAGACTCCTGCTTCTGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCgg                                                                                                                                                                                      >  1:307119/1‑251 (MQ=255)                                                        aagcaggCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAAGTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCtt                                                                                                                                                                                   <  1:366784/245‑1 (MQ=255)                                                           cgcggtcagggctgcGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAAGGCGGCACGGCGTTTGCCAATAATTTGGCGTTCAGCAGCAGCGGCAGCAGCTCTTCCAGCGCAGTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCGCGTCCATAGTCTGCCAGGTGTTACGCAGAAATTGGTCCAGTAACTGTTGCTCAAGCGCAAACGTAGACATCTCTTTGGCGGCTTTCa                                                                                                                                                                                <  2:354134/241‑1 (MQ=255)                                                                      ctgcGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCgt                                                                                                                                                                     >  1:268285/1‑250 (MQ=255)                                                                        gcGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCgtt                                                                                                                                                                    <  1:294635/250‑3 (MQ=255)                                                                        gcGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCAGTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCgtt                                                                                                                                                                    <  1:230468/250‑3 (MQ=255)                                                                                gCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACa                                                                                                                                                            <  1:120070/250‑1 (MQ=255)                                                                                gCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACAt                                                                                                                                                           <  1:339897/251‑1 (MQ=255)                                                                                             gTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAAt                                                                                                                                                                                                                >  1:195991/1‑185 (MQ=255)                                                                                             gTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAAt                                                                                                                                                                                                                <  2:195991/185‑1 (MQ=255)                                                                                               caattGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTg                                                                                                                                                                                                                                     >  2:116235/4‑162 (MQ=255)                                                                                               caattGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTg                                                                                                                                                                                                                                     <  1:116235/159‑1 (MQ=255)                                                                                                aattGGCTGGAAAGCAAATGCGACACGCCTTTTGCAAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCg                                                                                                                                           >  1:382089/3‑251 (MQ=255)                                                                                                             gCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCg                                                                                                                              >  2:455425/1‑251 (MQ=255)                                                                                                             gCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCg                                                                                                                              <  2:78823/251‑1 (MQ=255)                                                                                                                    cGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCcgc                                                                                                                       <  1:140460/251‑1 (MQ=255)                                                                                                                    cGACACGCCTTTTGCCAATAATTTGTCTGTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCcgc                                                                                                                       <  1:214014/251‑1 (MQ=255)                                                                                                                          gccTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTCGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGtt                                                                                                                 >  1:472798/2‑251 (MQ=255)                                                                                                                              tttGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGCTACAATTTACCGTGTCCGCGCAGTttgtt                                                                                                             >  2:171842/1‑251 (MQ=255)                                                                                                                                 gCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTgg                                                                                                           <  2:79742/250‑1 (MQ=255)                                                                                                                                 gCCAATAATTGGTCTTGCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTgg                                                                                                           <  1:340695/250‑1 (MQ=255)                                                                                                                                  ccAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCg                                                                                                         >  2:370454/1‑251 (MQ=255)                                                                                                                                    aataatTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGAt                                                                                                       <  1:171842/251‑1 (MQ=255)                                                                                                                                                      agcagcGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATg                                                                                     >  1:106108/1‑251 (MQ=255)                                                                                                                                                                         ccAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATATAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCGGCAATc                                                                 >  1:167415/1‑251 (MQ=255)                                                                                                                                                                            gCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCAGc                                                              <  1:10465/251‑1 (MQ=255)                                                                                                                                                                                     cccTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTg                                                     <  2:106108/251‑1 (MQ=255)                                                                                                                                                                                      ccTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTCGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTAGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTATTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTgc                                                    >  1:472092/1‑251 (MQ=255)                                                                                                                                                                                        tGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCGTATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATAAAATTTACCGTGTCCGCGCAGTTTGTTGGCACTACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTgcgg                                                  >  1:45803/1‑251 (MQ=255)                                                                                                                                                                                         ggCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGGCCATATTCTTCCAGGTGGTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGGAGACATCTCTTTGGCGGCTTTCAGCTTCAATCGTTTTGAAACAACGAGTAAAATGGCCCGATCCAATTTACCGTGTCCGCGCAGTTGGTTGGCGATACGATCGCCAGCAAAATG‑TGTTAATTCTCCAGCAATCAGCTGCCAGTTgcgg                                                  <  2:237337/250‑1 (MQ=255)                                                                                                                                                                                                aCCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCGTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTg                                          <  2:416483/251‑1 (MQ=255)                                                                                                                                                                                                       gCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCATCTGCCAGTTGCGGCGATGTTTCTCggg                                    >  1:177565/1‑250 (MQ=255)                                                                                                                                                                                                          gAAACTCCTGCTTATGGTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGc                                <  2:467312/251‑1 (MQ=255)                                                                                                                                                                                                           aaaCTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGcc                               >  2:209307/1‑251 (MQ=255)                                                                                                                                                                                                            accTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTACCTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGTCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATCCTATAGCCACCAAAATGCTGT‑CATTCTCCAGCAATTAGCTGCCAGTTACGGCGATGTTGTTAGTGATGccc                              >  2:377716/3‑251 (MQ=255)                                                                                                                                                                                                                ccTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTcc                          <  1:209307/251‑1 (MQ=255)                                                                                                                                                                                                                   gCTTATGTTCCTCGTACATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGCAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATAGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCAACCAAAACGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATc                       >  1:286349/1‑251 (MQ=255)                                                                                                                                                                                                                    cTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCg                      >  1:357090/1‑251 (MQ=255)                                                                                                                                                                                                                      tATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGAt                    >  2:41473/1‑251 (MQ=255)                                                                                                                                                                                                                            ccTCGTCAATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTCACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCCAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGCTACTATCGACACCAACATGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCCAGGGATGCCCTTCCATCACTTTaaa               >  2:353144/1‑250 (MQ=255)                                                                                                                                                                                                                                        tcttcCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCTCTTCCATCGATTTAAACAGTTCGTTgcgt  >  2:55003/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  TTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGT‑AATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGC  >  NC_000913/10744‑11225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑