Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,336	A→G	A53A (GCT→GCC)	satP ←	acetate/succinate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,336	0	A	G	100.0%	152.1 / NA	48	A53A (GCT→GCC)	satP	acetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/26);  total (22/26)

AACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATC  >  NC_000913/10091‑10578                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   aaCAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCaa                                                                                                                                                                                                                                                >  1:357985/1‑250 (MQ=255)              ggCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATg                                                                                                                                                                                                                                  >  1:40364/1‑251 (MQ=255)                            aaTTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGACCTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCTTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAAt                                                                                                                                                                                                                    <  1:451944/251‑1 (MQ=255)                                    aaCGCGTGGGCCTTTCAGCGTGACGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGAATTTGGCGCCTCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGCATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTCGAAAATGcgcaggg                                                                                                                                                                                                            >  2:27768/1‑245 (MQ=255)                                      cgcgTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGTAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCa                                                                                                                                                                                                          <  2:279688/251‑1 (MQ=255)                                      cgcgTGCGCCTTGCAGCGTGCCGAAGAACATAAACAGCGTAAAGACGCCCCCCAGACCCAGGTAGAAACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCa                                                                                                                                                                                                          <  2:266746/251‑1 (MQ=255)                                          tGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGa                                                                                                                                                                                                       >  2:381955/1‑250 (MQ=255)                                                 ttCAGCGTGCTGAAGAACATAAACAGCGTAAATACGCCACACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCAAGTTGAGGCAGCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAAGGCGGTTAAACCGAAAGGGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAataata                                                                                                                                                                                                 <  1:56208/249‑1 (MQ=255)                                                      cgggCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTcct                                                                                                                                                                                          <  2:91358/248‑2 (MQ=255)                                                       gTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGGGCATTTGGCGCAGCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCag                                                                                                                                                                                         <  1:253128/251‑1 (MQ=255)                                                         gCCGAAGAACATAAACAGCGTAAATAGCCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCACTTGGCGCAGCGGTCAGACCCAGGTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGGAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGGCCagag                                                                                                                                                                                       <  2:101197/251‑1 (MQ=255)                                                          ccGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGc                                                                                                                                                                                      <  2:40364/251‑1 (MQ=255)                                                                          gCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTtg                                                                                                                                                                      <  1:397184/251‑1 (MQ=255)                                                                                               ccAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCCGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGCAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTCGAAAATGCCCATGGCCAGAATAATACCGTCCAGAGCGCAATAACCCACGTTGTGCAGGTTAAGCAGAAtgggg                                                                                                                                                 >  2:484727/1‑249 (MQ=255)                                                                                               ccAGGTAGACACCAAGGAACTGTGCATTTGGCGCAACGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAAtggtg                                                                                                                                                 <  1:414197/251‑1 (MQ=255)                                                                                                  ggTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTc                                                                                                                                              >  1:304300/1‑251 (MQ=255)                                                                                                           cAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGAGTTGCCTTTTTTGTACTACAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAACTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGcaga                                                                                                                                       >  2:209636/1‑246 (MQ=255)                                                                                                              ggaactgggccttgggcgctctgggcAGATCCATTTTCGGTATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGACCCGTAGGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTTCTCCAGCAGACCGTCAAAAATTTGCGGGATGCCGCCGTAGAAAATGCCCTTGGCAAGAATAATACCGTCCAGAGCGAAATGACCCACGTTGTGCAGGTTAAGCAGAATGGCGGTCATGCCGAAGccc                                                                                                                                  <  2:435190/226‑1 (MQ=255)                                                                                                                          ttGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATg                                                                                                                                           >  2:427252/1‑230 (MQ=255)                                                                                                                          ttGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATg                                                                                                                                           <  1:427252/230‑1 (MQ=255)                                                                                                                                      ccAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTa                                                                                                          <  2:190157/250‑1 (MQ=255)                                                                                                                                       cAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTGTTTTGTACTCCAGCAGACCGGCAAAAAGTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAg                                                                                                         <  1:291488/251‑1 (MQ=255)                                                                                                                                             aaGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAAGGCGTTTAAACCTAAAGTGTTGCCTTTTTTGTACTCCAGCAGCCCGGAAAAAATGTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGGCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACt                                                                                                   <  2:13031/250‑1 (MQ=255)                                                                                                                                                    ggCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGtt                                                                                            <  1:431460/251‑1 (MQ=255)                                                                                                                                                     gCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGCAATAACCCACGTTGTGCAGGTTAAGCAGAAAGGTGGTCATGCCGAAGCCCACCAGGCCCAGCGGTGACGGATTAGACAACTTAGGGTTg                                                                                           >  2:120070/1‑251 (MQ=255)                                                                                                                                                       aTCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGcc                                                                                         >  2:166658/1‑251 (MQ=255)                                                                                                                                                         cagcaaAACCGCAACCAACGTCAGCCAGAAAGAACCGGAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTTTACTCCAGCAGACCGGCAAAAATTGGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGTATTAGCCAACTTAGTGTTGccc                                                                                        <  2:296798/250‑1 (MQ=255)                                                                                                                                                             agAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAt                                                                                   >  1:3518/1‑251 (MQ=255)                                                                                                                                                                        caacGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAACTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCCCGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGGGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAat                                                                        >  2:165922/4‑251 (MQ=255)                                                                                                                                                                             gcAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACCCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAAGACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatca                                                                   <  2:153974/250‑1 (MQ=255)                                                                                                                                                                               aGCCAGAAAGAACCGTAAGAGGTGAATGCGTTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcatc                                                                 <  1:182863/251‑1 (MQ=255)                                                                                                                                                                                        gaaCCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGaatgaatg                                                        <  1:344237/251‑1 (MQ=255)                                                                                                                                                                                                agagGAGAATGCCGTTAAACCGCAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAACATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGCTTAGCCAACTTAGTGTTGCCCTTACTTCCTCACAAATCATCATCGAATGAATGGTGAaat                                                 >  2:214014/1‑250 (MQ=255)                                                                                                                                                                                                    gTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCCTAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAAttt                                            >  2:10142/1‑251 (MQ=255)                                                                                                                                                                                                         tGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCa                                                                                                      >  1:257184/1‑188 (MQ=255)                                                                                                                                                                                                         tGCGGTTAAACCGAAAGGGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCa                                                                                                      <  2:257184/188‑1 (MQ=255)                                                                                                                                                                                                           cGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGa                                      >  2:324326/1‑250 (MQ=255)                                                                                                                                                                                                               tAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCGGAATaa                                  <  1:190326/250‑1 (MQ=255)                                                                                                                                                                                                                aaaCCGAAAGTGTGGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATTCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAAGCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAca                                <  2:186723/251‑2 (MQ=255)                                                                                                                                                                                                                  aCCGAAAGTTTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAcagt                              <  1:10142/251‑4 (MQ=255)                                                                                                                                                                                                                  aCCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAc                                 >  2:255975/1‑248 (MQ=255)                                                                                                                                                                                                                     gAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACagtagt                           <  1:381955/251‑1 (MQ=255)                                                                                                                                                                                                                       aaGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAgtgt                         >  1:394315/1‑251 (MQ=255)                                                                                                                                                                                                                          tgtTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCCAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGACTGAATGGTGAAATAATTTCACTGAATAACAGTAGTGtttt                      >  2:294635/1‑251 (MQ=255)                                                                                                                                                                                                                          tgtTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGGGAAATAATTTCCCTGAATAACAGTAGTGtttt                      >  1:319641/1‑251 (MQ=255)                                                                                                                                                                                                                              gCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAgg                  <  2:176041/251‑1 (MQ=255)                                                                                                                                                                                                                                          cTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGGGTTTTCAGGGCGAGGCataa       >  1:148174/1‑250 (MQ=255)                                                                                                                                                                                                                                              agcagACCGGCAAAAATTTGCGCGATGCCGCCGTAGACAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGAGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGCGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATc  >  2:25039/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   AACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATC  >  NC_000913/10091‑10578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑