Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 10,260 G→A L79L (CTG→TTG)  satP ← acetate/succinate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091310,2600GA97.9% 147.6 / NA 47L79L (CTG→TTG) satPacetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/28);  minor base C (1/0);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAT  >  NC_000913/10013‑10497
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             
ccacAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCTCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACTCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGt                                                                                                                                                                                                                                            <  1:321023/248‑1 (MQ=255)
      atcagaccactacAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTCAAGAAAACGAATTGCAGAACGCGTGAGCCTTTCAGCGTGCCGATGCACATACACAGCGTAAATACGCCCCACAGACCCAGGTATGCACCAAGGAACTGTGCATTTGGCGCATCGGGCAGACCCAGTTTCGGCATCAGCAGAATCGAAACCAACGTCAGcc                                                                                                                                                                                                                                       >  2:447267/13‑250 (MQ=255)
             cAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTACAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGACTCGCAACCAAAGTCAGCCagaaaga                                                                                                                                                                                                                                >  1:293091/1‑250 (MQ=255)
              aaTCAAGCCGGAAAAGTGGATGATTGCGGCGTTACCGGAAGTGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCCGGCTAAAGAAAACGAAATGCAGAACGCGTGCGCCTTTCAGCGAGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACACAGGGAACCACCAGGGAACTGTGCATTTGGCGCATCGGTCAAACCCACTTTCAGCATCAGCAGGATCGCAACCACCGTCAGCCAGAAAGAAc                                                                                                                                                                                                                              >  2:155898/1‑251 (MQ=255)
                accAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCg                                                                                                                                                                                                                            <  1:108776/250‑1 (MQ=255)
                 ccAGCCGGCAAAGTGGATGATTGAGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTCAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAAAAGCGTAAATACGCCCCACAGCACCAGGTAGACACCAACGAACTGTGCATTTGGCGCATCGGTCGGCCCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCAAGACAGAACCgg                                                                                                                                                                                                                           >  2:182863/1‑250 (MQ=255)
                       ggCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAgg                                                                                                                                                                                                                     <  2:168033/251‑1 (MQ=255)
                                    atTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCACACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGAATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTa                                                                                                                                                                                                         >  1:186723/1‑250 (MQ=255)
                                       gcggcgTTACCGGCAATGGTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGGGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCTGCATCAGCAGAATCGCAACCAACGTCAGTCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAAcc                                                                                                                                                                                                     <  2:31307/251‑1 (MQ=255)
                                          gcgTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGaa                                                                                                                                                                                                  <  1:233595/251‑1 (MQ=255)
                                               aCCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAgtgt                                                                                                                                                                                             <  1:59016/251‑1 (MQ=255)
                                               aCCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAgtgt                                                                                                                                                                                             >  1:465662/1‑251 (MQ=255)
                                               aCCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGGGAATGCGGTTAAACCGAAAgtgt                                                                                                                                                                                             >  1:190157/1‑251 (MQ=255)
                                                  ggCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGc                                                                                                                                                                                          <  1:348910/251‑1 (MQ=255)
                                                    cAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCt                                                                                                                                                                                        <  1:222251/251‑1 (MQ=255)
                                                     aaTGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCtt                                                                                                                                                                                       <  1:207717/251‑1 (MQ=255)
                                                          tACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCTCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCTCCACAGGCCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGATGTGAATGCGGTTAAACCGAAAGTGTTTCCtttttt                                                                                                                                                                                   <  1:308898/250‑1 (MQ=255)
                                                           aCCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTg                                                                                                                                                                                  <  2:196703/250‑1 (MQ=255)
                                                              gATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACt                                                                                                                                                                              >  1:153974/1‑251 (MQ=255)
                                                                              aaCAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCaa                                                                                                                                                               >  1:357985/1‑250 (MQ=255)
                                                                                           ggCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATg                                                                                                                                                 >  1:40364/1‑251 (MQ=255)
                                                                                                         aaTTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGACCTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCTTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAAt                                                                                                                                   <  1:451944/251‑1 (MQ=255)
                                                                                                                 aaCGCGTGGGCCTTTCAGCGTGACGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGAATTTGGCGCCTCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGCATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTCGAAAATGcgcaggg                                                                                                                           >  2:27768/1‑245 (MQ=255)
                                                                                                                   cgcgTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGTAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCa                                                                                                                         <  2:279688/251‑1 (MQ=255)
                                                                                                                   cgcgTGCGCCTTGCAGCGTGCCGAAGAACATAAACAGCGTAAAGACGCCCCCCAGACCCAGGTAGAAACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCa                                                                                                                         <  2:266746/251‑1 (MQ=255)
                                                                                                                       tGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGa                                                                                                                      >  2:381955/1‑250 (MQ=255)
                                                                                                                              ttCAGCGTGCTGAAGAACATAAACAGCGTAAATACGCCACACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCAAGTTGAGGCAGCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAAGGCGGTTAAACCGAAAGGGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAataata                                                                                                                <  1:56208/249‑1 (MQ=255)
                                                                                                                                   cgggCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTcct                                                                                                         <  2:91358/248‑2 (MQ=255)
                                                                                                                                    gTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGGGCATTTGGCGCAGCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCag                                                                                                        <  1:253128/251‑1 (MQ=255)
                                                                                                                                      gCCGAAGAACATAAACAGCGTAAATAGCCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCACTTGGCGCAGCGGTCAGACCCAGGTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGGAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGGCCagag                                                                                                      <  2:101197/251‑1 (MQ=255)
                                                                                                                                       ccGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGc                                                                                                     <  2:40364/251‑1 (MQ=255)
                                                                                                                                                       gCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTtg                                                                                     <  1:397184/251‑1 (MQ=255)
                                                                                                                                                                            ccAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCCGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGCAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTCGAAAATGCCCATGGCCAGAATAATACCGTCCAGAGCGCAATAACCCACGTTGTGCAGGTTAAGCAGAAtgggg                                                                >  2:484727/1‑249 (MQ=255)
                                                                                                                                                                            ccAGGTAGACACCAAGGAACTGTGCATTTGGCGCAACGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAAtggtg                                                                <  1:414197/251‑1 (MQ=255)
                                                                                                                                                                               ggTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTc                                                             >  1:304300/1‑251 (MQ=255)
                                                                                                                                                                                        cAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGAGTTGCCTTTTTTGTACTACAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAACTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGcaga                                                      >  2:209636/1‑246 (MQ=255)
                                                                                                                                                                                           ggaactgggccttgggcgctctgggcAGATCCATTTTCGGTATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGACCCGTAGGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTTCTCCAGCAGACCGTCAAAAATTTGCGGGATGCCGCCGTAGAAAATGCCCTTGGCAAGAATAATACCGTCCAGAGCGAAATGACCCACGTTGTGCAGGTTAAGCAGAATGGCGGTCATGCCGAAGccc                                                 <  2:435190/226‑1 (MQ=255)
                                                                                                                                                                                                       ttGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATg                                                          <  1:427252/230‑1 (MQ=255)
                                                                                                                                                                                                       ttGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATg                                                          >  2:427252/1‑230 (MQ=255)
                                                                                                                                                                                                                   ccAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTa                         <  2:190157/250‑1 (MQ=255)
                                                                                                                                                                                                                    cAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTGTTTTGTACTCCAGCAGACCGGCAAAAAGTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAg                        <  1:291488/251‑1 (MQ=255)
                                                                                                                                                                                                                          aaGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAAGGCGTTTAAACCTAAAGTGTTGCCTTTTTTGTACTCCAGCAGCCCGGAAAAAATGTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGGCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACt                  <  2:13031/250‑1 (MQ=255)
                                                                                                                                                                                                                                 ggCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGtt           <  1:431460/251‑1 (MQ=255)
                                                                                                                                                                                                                                  gCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGCAATAACCCACGTTGTGCAGGTTAAGCAGAAAGGTGGTCATGCCGAAGCCCACCAGGCCCAGCGGTGACGGATTAGACAACTTAGGGTTg          >  2:120070/1‑251 (MQ=255)
                                                                                                                                                                                                                                    aTCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGcc        >  2:166658/1‑251 (MQ=255)
                                                                                                                                                                                                                                      cagcaaAACCGCAACCAACGTCAGCCAGAAAGAACCGGAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTTTACTCCAGCAGACCGGCAAAAATTGGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGTATTAGCCAACTTAGTGTTGccc       <  2:296798/250‑1 (MQ=255)
                                                                                                                                                                                                                                          agAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAt  >  1:3518/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             
CCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAT  >  NC_000913/10013‑10497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑