Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,996	G→T	A253A (GCG→GCT)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,996	0	G	T	95.6%	140.3 / ‑1.6	45	A253A (GCG→GCT)	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (17/26);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCT  >  NC_000913/8762‑9231                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            ctcGCCGTTTGTTGGCCGTATTCTTGACTGGTACACAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGCAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCt                                                                                                                                                                                                                              >  1:165044/1‑250 (MQ=255)   cGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAAGACCGATAAGAAATAGTACGCTCCGGCAGAAGATCCGGGCGTGGTGTCTGTATCTGAAATCTACCAGTACTCCAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATc                                                                                                                                                                                                                           <  2:323148/251‑1 (MQ=255)              ggCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACt                                                                                                                                                                                                                <  1:220604/251‑1 (MQ=255)                    aTTCTTGCCTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCGCCTGCAGAAGATCCGGGCGTGGTGTCGGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCTACCGTCTTCCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAATGGGCTATCGAACGTAAACTGTCtt                                                                                                                                                                                                           <  2:186177/250‑1 (MQ=255)                            cTGGTACAAAGCGAACACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCg                                                                                                                                                                                                  >  1:90466/1‑251 (MQ=255)                                 aCAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTg                                                                                                                                                                                             <  2:90466/251‑1 (MQ=255)                                    aaGCGAAGACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTTCTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGaaa                                                                                                                                                                                          <  2:195151/251‑1 (MQ=255)                                          aTACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGAGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGATATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCCACTACACTGCTGAAAGAGCTGGCTGGGCGGGAAGGGGCTATCGAACGTTAACTGTCTTACACCGGCGAGGTGAAATcgcgc                                                                                                                                                                                    >  2:193141/1‑250 (MQ=255)                                              cGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCgg                                                                                                                                                                                <  2:436554/251‑1 (MQ=255)                                               gATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCCCTGCTGAAAGAACTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAATGAAAGCGCGTCCGgc                                                                                                                                                                               >  2:191174/1‑251 (MQ=255)                                                aTAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGCGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGCCCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAAGGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGGGCGGCCGgcg                                                                                                                                                                              >  2:25694/1‑251 (MQ=255)                                                aTAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACCGTCTTACACCGGCGAAGTGAAAGCGAGTCCGgcg                                                                                                                                                                              >  2:421796/1‑251 (MQ=255)                                                     aaagaGTACGCTTCCGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAATAGCACTGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACTTCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCTCACCTTCACGGCTTAAAGAGCTCGCTGAGAGCTAATTGTCTATCGAACGTAAACTGTCTTACACCGTCGAAGTGAAAGCTCGTCCGGCGCTTAt                                                                                                                                                                         <  1:298982/251‑1 (MQ=255)                                                     aaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCCCCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTAt                                                                                                                                                                         <  1:298987/251‑1 (MQ=255)                                                     aaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTAt                                                                                                                                                                         <  1:476703/251‑1 (MQ=255)                                                                 ccGGCAGAATAGCCGGGCGTGGTTTCTGTATCTGAAAGCTACCAGTACTTCAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGa                                                                                                                                                             <  2:326150/251‑1 (MQ=255)                                                                   gggagaagaGCCGGGCGTGGTGTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGt                                                                                                                                                           <  2:26130/248‑1 (MQ=255)                                                                   ggCAGAAGATCCGGACGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGAAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGt                                                                                                                                                           >  1:140505/1‑251 (MQ=255)                                                                                         tCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAgg                                                                                                                                      <  2:251446/250‑1 (MQ=255)                                                                                                  taaaTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCTCAAGCTTCCGTAACAACGGCGAAATCCTGGAACTGGCAGGCTGCGGCCGTCGTACCATCGCACCTACACTGCGGAAAGAGCGGGCTGAGAGCGAAGGGGCTATCGAACGGAATCTGTCTGACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGGCCGAGTTCCTGTGGCAGCACAACGAGTATCCAATg                                                                                                                              <  2:244096/248‑1 (MQ=255)                                                                                                     aTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCCGCACAACCAGGATCCAATGGCAGt                                                                                                                         >  1:68599/1‑251 (MQ=255)                                                                                                         aCCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTa                                                                                                                        <  1:21200/248‑1 (MQ=255)                                                                                                               actacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCACGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGCAGGGGCTATCGAACGTAACCTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTg                                                                                                               >  2:34408/1‑251 (MQ=255)                                                                                                               actacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGCCCGTCTTACCATCGCACCTACCCTGCTGAAAGAGCTGGCTGAGAGAGAAGGCGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGGTCCCATGGCAATAGATAAACTg                                                                                                               >  2:186442/1‑251 (MQ=255)                                                                                                                   cAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCgg                                                                                                           >  1:102627/1‑251 (MQ=255)                                                                                                                               ttATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAg                                                                                                                                                                                         <  2:20434/161‑1 (MQ=255)                                                                                                                               ttATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAg                                                                                                                                                                                         >  1:20434/1‑161 (MQ=255)                                                                                                                                taggAAACCGTGGTTATGGGTGCAAGCTTCCGTAACATCGGCGAAATTCAGGAACTGGCAGGCTGCGGCCGTCTTACCATCTCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGTCGATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACATCCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATccttt                                                                                               <  2:159300/247‑4 (MQ=255)                                                                                                                                   gAAACCGTGGTTATTGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGtt                                                                                           <  1:267424/251‑1 (MQ=255)                                                                                                                                           ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCCCAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAATTTGCTAttt                                                                                   >  2:117261/1‑250 (MQ=255)                                                                                                                                           ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTg                                                                                   <  2:214487/251‑1 (MQ=255)                                                                                                                                           ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTg                                                                                   >  1:91547/1‑251 (MQ=255)                                                                                                                                               aTGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAcc                                                                                <  1:390076/250‑1 (MQ=255)                                                                                                                                                    cgcAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaa                                                                          <  1:117261/251‑1 (MQ=255)                                                                                                                                                     gcAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTCCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCGTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGGTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGGGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaaa                                                                         <  2:394905/251‑1 (MQ=255)                                                                                                                                                                               tCTGGAACTGTCATGCTGCGTCCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGGTCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCTGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctg                                                <  1:218888/250‑1 (MQ=255)                                                                                                                                                                                     aCTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGCGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct                                                 >  1:164552/1‑243 (MQ=255)                                                                                                                                                                                     aCTGGCAGGCTGCGACCGTCGTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGGTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct                                                 <  2:164552/243‑1 (MQ=255)                                                                                                                                                                                        ggCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCa                                       <  1:265414/250‑1 (MQ=255)                                                                                                                                                                                                       tcttaCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAAATGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCgg                       >  2:332863/5‑251 (MQ=255)                                                                                                                                                                                                          taCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGa                     >  1:142557/2‑250 (MQ=255)                                                                                                                                                                                                           aCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGa                     >  1:234137/1‑249 (MQ=255)                                                                                                                                                                                                             cATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTc                 <  2:102627/251‑1 (MQ=255)                                                                                                                                                                                                             cATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATACTTCTTAACGTGACCGGGCAGTc                 >  2:135568/1‑251 (MQ=255)                                                                                                                                                                                                                            gctgAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACctct  <  1:332863/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            CTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCT  >  NC_000913/8762‑9231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑