Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,963	G→T	L242L (CTG→CTT)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,963	0	G	T	93.9%	145.9 / ‑0.7	49	L242L (CTG→CTT)	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (21/25);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCA  >  NC_000913/8716‑9194                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        ccgtCGCTCAGGGTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAAGACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTAGGGGCGCAAGCTTCCGTAACATAGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTggcc                                                                                                                                                                                                                                      <  1:226560/248‑4 (MQ=255)          aGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCAcct                                                                                                                                                                                                                             <  1:394283/251‑2 (MQ=255)                gTGCTTGTGCGGAAGCGTGCGTGTTCCTGCTCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGCAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCAcctacactg                                                                                                                                                                                                                       >  1:34880/1‑244 (MQ=255)                 tGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGAACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGATTCTGTATATGAAATCTACCAGTACTACCAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGTCTGCGACCGACTTACCATCGCACCTACActgc                                                                                                                                                                                                                      >  1:465287/1‑251 (MQ=255)                    ttGTGCGGACGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGGGGTTTCTGTATCTGAAATCTACCAGTCCTACACAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTACGTAACATCGGCGACATTCTTGAACTGGCCGGCTGCGACCGTCTTACCATCggcaccaccctgatgg                                                                                                                                                                                                                   >  2:251074/1‑236 (MQ=255)                    ttGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCAGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCAcctacacggctg                                                                                                                                                                                                                    >  2:234973/1‑240 (MQ=255)                      gtgCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGaa                                                                                                                                                                                                                  >  1:247978/1‑250 (MQ=255)                          ggAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAagcgc                                                                                                                                                                                                             >  1:202619/1‑248 (MQ=255)                                            gATCTCACCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGCAAGAGCTGGctgcaagcgaggggg                                                                                                                                                                                           >  2:47307/1‑237 (MQ=255)                                               ctcGCCGTTTGTTGGCCGTATTCTTGACTGGTACACAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGCAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCt                                                                                                                                                                                         >  1:165044/1‑250 (MQ=255)                                                 cGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAAGACCGATAAGAAATAGTACGCTCCGGCAGAAGATCCGGGCGTGGTGTCTGTATCTGAAATCTACCAGTACTCCAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATc                                                                                                                                                                                      <  2:323148/251‑1 (MQ=255)                                                  gCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAaga                                                                                                                                                                                                               >  1:253323/1‑225 (MQ=255)                                                  gCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAaga                                                                                                                                                                                                               <  2:253323/225‑1 (MQ=255)                                                            ggCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACt                                                                                                                                                                           <  1:220604/251‑1 (MQ=255)                                                                  aTTCTTGCCTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCGCCTGCAGAAGATCCGGGCGTGGTGTCGGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCTACCGTCTTCCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAATGGGCTATCGAACGTAAACTGTCtt                                                                                                                                                                      <  2:186177/250‑1 (MQ=255)                                                                          cTGGTACAAAGCGAACACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCg                                                                                                                                                             >  1:90466/1‑251 (MQ=255)                                                                               aCAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTg                                                                                                                                                        <  2:90466/251‑1 (MQ=255)                                                                                  aaGCGAAGACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTTCTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGaaa                                                                                                                                                     <  2:195151/251‑1 (MQ=255)                                                                                        aTACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGAGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGATATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCCACTACACTGCTGAAAGAGCTGGCTGGGCGGGAAGGGGCTATCGAACGTTAACTGTCTTACACCGGCGAGGTGAAATcgcgc                                                                                                                                               >  2:193141/1‑250 (MQ=255)                                                                                            cGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCgg                                                                                                                                           <  2:436554/251‑1 (MQ=255)                                                                                             gATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCCCTGCTGAAAGAACTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAATGAAAGCGCGTCCGgc                                                                                                                                          >  2:191174/1‑251 (MQ=255)                                                                                              aTAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGCGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGCCCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAAGGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGGGCGGCCGgcg                                                                                                                                         >  2:25694/1‑251 (MQ=255)                                                                                              aTAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACCGTCTTACACCGGCGAAGTGAAAGCGAGTCCGgcg                                                                                                                                         >  2:421796/1‑251 (MQ=255)                                                                                                   aaagaGTACGCTTCCGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAATAGCACTGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACTTCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCTCACCTTCACGGCTTAAAGAGCTCGCTGAGAGCTAATTGTCTATCGAACGTAAACTGTCTTACACCGTCGAAGTGAAAGCTCGTCCGGCGCTTAt                                                                                                                                    <  1:298982/251‑1 (MQ=255)                                                                                                   aaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCCCCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTAt                                                                                                                                    <  1:298987/251‑1 (MQ=255)                                                                                                   aaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTAt                                                                                                                                    <  1:476703/251‑1 (MQ=255)                                                                                                               ccGGCAGAATAGCCGGGCGTGGTTTCTGTATCTGAAAGCTACCAGTACTTCAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGa                                                                                                                        <  2:326150/251‑1 (MQ=255)                                                                                                                 gggagaagaGCCGGGCGTGGTGTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGt                                                                                                                      <  2:26130/248‑1 (MQ=255)                                                                                                                 ggCAGAAGATCCGGACGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGAAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGt                                                                                                                      >  1:140505/1‑251 (MQ=255)                                                                                                                                       tCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAgg                                                                                                 <  2:251446/250‑1 (MQ=255)                                                                                                                                                taaaTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCTCAAGCTTCCGTAACAACGGCGAAATCCTGGAACTGGCAGGCTGCGGCCGTCGTACCATCGCACCTACACTGCGGAAAGAGCGGGCTGAGAGCGAAGGGGCTATCGAACGGAATCTGTCTGACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGGCCGAGTTCCTGTGGCAGCACAACGAGTATCCAATg                                                                                         <  2:244096/248‑1 (MQ=255)                                                                                                                                                   aTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCCGCACAACCAGGATCCAATGGCAGt                                                                                    >  1:68599/1‑251 (MQ=255)                                                                                                                                                       aCCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTa                                                                                   <  1:21200/248‑1 (MQ=255)                                                                                                                                                             actacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCACGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGCAGGGGCTATCGAACGTAACCTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTg                                                                          >  2:34408/1‑251 (MQ=255)                                                                                                                                                             actacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGCCCGTCTTACCATCGCACCTACCCTGCTGAAAGAGCTGGCTGAGAGAGAAGGCGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGGTCCCATGGCAATAGATAAACTg                                                                          >  2:186442/1‑251 (MQ=255)                                                                                                                                                                 cAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCgg                                                                      >  1:102627/1‑251 (MQ=255)                                                                                                                                                                             ttATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAg                                                                                                                                                    <  2:20434/161‑1 (MQ=255)                                                                                                                                                                             ttATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAg                                                                                                                                                    >  1:20434/1‑161 (MQ=255)                                                                                                                                                                              taggAAACCGTGGTTATGGGTGCAAGCTTCCGTAACATCGGCGAAATTCAGGAACTGGCAGGCTGCGGCCGTCTTACCATCTCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGTCGATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACATCCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATccttt                                                          <  2:159300/247‑4 (MQ=255)                                                                                                                                                                                 gAAACCGTGGTTATTGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGtt                                                      <  1:267424/251‑1 (MQ=255)                                                                                                                                                                                         ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCCCAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAATTTGCTAttt                                              >  2:117261/1‑250 (MQ=255)                                                                                                                                                                                         ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTg                                              <  2:214487/251‑1 (MQ=255)                                                                                                                                                                                         ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTg                                              >  1:91547/1‑251 (MQ=255)                                                                                                                                                                                             aTGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAcc                                           <  1:390076/250‑1 (MQ=255)                                                                                                                                                                                                  cgcAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaa                                     <  1:117261/251‑1 (MQ=255)                                                                                                                                                                                                   gcAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTCCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCGTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGGTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGGGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaaa                                    <  2:394905/251‑1 (MQ=255)                                                                                                                                                                                                                             tCTGGAACTGTCATGCTGCGTCCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGGTCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCTGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctg           <  1:218888/250‑1 (MQ=255)                                                                                                                                                                                                                                   aCTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGCGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct            >  1:164552/1‑243 (MQ=255)                                                                                                                                                                                                                                   aCTGGCAGGCTGCGACCGTCGTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGGTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct            <  2:164552/243‑1 (MQ=255)                                                                                                                                                                                                                                      ggCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCa  <  1:265414/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        CCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCA  >  NC_000913/8716‑9194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑