Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,365,526	IS2 (–) +5 bp	coding (1244‑1248/1302 nt)	dcuA ←	C4‑dicarboxylate transporter DcuA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	49 (1.070)	45/478	0.1	96.0%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	4365526 =	2 (0.040)					coding (1248/1302 nt)	dcuA	C4‑dicarboxylate transporter DcuA
*	?	NC_000913	= 3187426	NA (NA)	52 (1.140)	46/476	0.1	96.2%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	= 4365530	2 (0.040)					coding (1244/1302 nt)	dcuA	C4‑dicarboxylate transporter DcuA

GGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACATTATTAGGATTT  >  NC_000913/3187178‑3187675                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                          ggTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACTGGTTAACGCCAGCAAAGACCCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTTGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGTGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGt                                                                                                                                                                                                                                                         <  1:61923/251‑1 (MQ=2)            cGGATAGTAACGTAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAATCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGTCATCCGCATAGTGCGCTGGGTTATCTCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTg                                                                                                                                                                                                                                              <  1:451942/251‑1 (MQ=255)                    aaCGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAATTCCAGTTATCATCATTGGTTGTGCg                                                                                                                                                                                                                                      <  2:317100/251‑1 (MQ=38)                       ggAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTc                                                                                                                                                                                                                                    >  2:345481/1‑250 (MQ=40)                               agagagCTTCGTGAAAACGAGAAAGCGTTACTACATCAGTATCATGCCCAAACCAGACGGGTAAACGGCAGAAAAGAACCTGGCAGAGGCGGTCGAGCATTAGAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAAGATTGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGc                                                                                                                                                                                                                           <  2:363588/251‑1 (MQ=14)                                 agagCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCg                                                                                                                                                                                                                         <  1:449625/251‑1 (MQ=255)                                  gagCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGAGTTCGAGCATTATAACGACTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATACAAGATGTCTGGAAATATAGGGGCACATCCAGCTATCATCATTGGTTGTGCGTCAGCTTATGCCGt                                                                                                                                                                                                                        >  1:67482/1‑251 (MQ=255)                                                cGATAAAGAGTGACTACAACAGTATCATGCCCAAACCAGATGGGGTAACGGCAGCAAAGAACCTTGCAGAGGCGGTCGAGCATTATAACGAATGGCATCCGCAGAGTGCGCTGGGTGATCGCGCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATTCCGTTGAATTCAACAAAg                                                                                                                                                                                                          <  2:88832/251‑1 (MQ=17)                                                  aTAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGTATCATCATTGGTTGGGCGTCAGCTTATGCCGTTGAATTCAACAAAGAt                                                                                                                                                                                                        <  1:164654/251‑1 (MQ=255)                                                      aGCGTGACTACATCAGGATCATTCCCGAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGGGGCGTTCGAGCATTATAACGAATGGCATCCGGATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGGTAAGGGATAACAGAGGTCTGGAAATAGAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTGATGCCGTTGAATTCAACAAAGATTTaa                                                                                                                                                                                                    <  2:476809/251‑1 (MQ=17)                                                       gCGTGACTACATCATTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAAt                                                                                                                                                                                                   <  1:35721/251‑1 (MQ=255)                                                              tACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTg                                                                                                                                                                                                                                              <  2:140295/201‑1 (MQ=35)                                                              tACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGGAATGGGTTAAGTGATAACAGATGTCTGGGAATATAGGGGCAAATCCAGTTATCATCATTg                                                                                                                                                                                                                                              >  1:140295/1‑201 (MQ=25)                                                                              ccAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCCTCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCACCAAAGATTTAATCGAAGCCGCAGATCGTGAAAACg                                                                                                                                                                            >  2:137575/1‑251 (MQ=255)                                                                                 aaCCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGtt                                                                                                                                                                          >  1:411301/1‑250 (MQ=255)                                                                                     aGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCt                                                                                                                                                                     <  1:137575/251‑1 (MQ=255)                                                                                          ggTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCAGCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAAGCGAAGCCGAAGATCGTGAAAACGTTAACCTTTccc                                                                                                                                                                <  2:472314/251‑1 (MQ=255)                                                                                                cGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCAGCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTg                                                                                                                                                          <  2:279200/251‑1 (MQ=255)                                                                                                 ggctgcaAAGCACCCTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACTGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCTTTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTACCCTTTCCCAATTTGa                                                                                                                                                         <  2:136987/247‑1 (MQ=255)                                                                                                  gcagcaAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGATAACGTTAACCTTTCCCAATTTGaa                                                                                                                                                        <  2:191413/251‑1 (MQ=255)                                                                                                    agcaAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCAGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAAc                                                                                                                                                      >  2:177620/1‑251 (MQ=255)                                                                                                            aCCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCg                                                                                                                                                                                                 >  2:163523/1‑200 (MQ=255)                                                                                                            aCCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCg                                                                                                                                                                                                 <  1:163523/200‑1 (MQ=255)                                                                                                            aCCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGcc                                                                                                                                              <  1:9856/251‑1 (MQ=255)                                                                                                             cctagcgtatgCGTTCTAGCATTATCACTAATGGCAGTCGCATAGTGCGCTGGGTTATCGCTCGTCTCGGGAATATCTGCGGTAGCGGGCTTGTTATGGGATAAGTGATAACAGATGTATGGAAATAGAGGGTCAAATCCAGTTATCGTCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGCAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGATACTGATGGCCa                                                                                                                                             <  2:417009/241‑1 (MQ=255)                                                                                                                   agagGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTAcc                                                                                                                                       >  2:381609/1‑251 (MQ=255)                                                                                                                            cGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCa                                                                                                                               >  2:186650/1‑250 (MQ=255)                                                                                                                                   tataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTc                                                                                                                        <  1:177620/250‑1 (MQ=255)                                                                                                                                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGAGGGCCAATTACCCGTCGGCAAATATTCa                                                                                                                       <  1:375738/250‑1 (MQ=255)                                                                                                                                          aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCa                                                                                                                <  1:404884/251‑1 (MQ=255)                                                                                                                                                  ccGCATAGGGCGCTGTGTTATCGATCGCAACGGGAATATCTGTGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGTATCATCATTGTTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATTGAAGCCGAAGATCGTGAAACCGTTAACCTTTCCCAATCTGAAACTGATGGCCAATTACCCGGCGGCAAATATTCACTAAGCACTCTGAt                                                                                                         <  2:250246/250‑1 (MQ=37)                                                                                                                                                        aGTGCGCTGGGTTATCGCTCTCCACGGGAATATCTGCGGCAGCGGTCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACGAAGCACTCTGATtaataa                                                                                                   <  1:317168/250‑1 (MQ=255)                                                                                                                                                             gcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAag                                                                                               >  1:124978/1‑249 (MQ=255)                                                                                                                                                                 ggTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACg                                                                                         <  1:186650/251‑1 (MQ=255)                                                                                                                                                                        gctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGAGGGCTGGAAATATAGGGGCAAATCCAGTTAACATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATcc                                                                                  <  2:44053/251‑1 (MQ=255)                                                                                                                                                                                 gggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGAcc                                                                         >  1:328358/1‑251 (MQ=255)                                                                                                                                                                                     atatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAACCATAGGGGCAAATACAGTTATCATCATTGGTTGTGCGTCAGCTTAAGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATAGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCAGTCTACAAATATTCACTAAGCACTCTGATTCATAATAAGTGGACGCAACTCCACCTTGAccacc                                                                      >  2:104756/1‑247 (MQ=38)                                                                                                                                                                                             ggCAGCGGGCTTGTAATGGGTTAAGTGATAAAAGATGTCTGGGAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGTTGGCCAATTACCCGTCGGCAACTATTCACTAAGCACTCTGATTAATAATAAGAGGCCGCCAATCCACCTTGACCTCCCATGGGTAt                                                             >  2:105021/1‑251 (MQ=255)                                                                                                                                                                                                    ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGa                                                      <  1:398311/251‑1 (MQ=255)                                                                                                                                                                                                      cTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGAt                                                     >  1:169888/1‑250 (MQ=255)                                                                                                                                                                                                          tAATGGGTTAAGTGATAACAGATGTCTGGAAATCTAGGGGCAAATCCAGTTATCAAAATTGATTGTGCGTCAGCTTATGACGATGAATTCAACAAAGATTTACTCGAAGCCGAAGATCGCGAAAAAGTTAACCTTTCCCAATTTGAAACTGATCGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATGAATAATACGTGGACGCCAATCCACCTTGCCCTCCAATGGGTACTAATTGATAAcc                                                 >  1:208030/1‑250 (MQ=255)                                                                                                                                                                                                              gggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCATCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGAAGGCCAATTACCCGTCGGCAAATATTCACTAAGCACCCTGATTAATAATAAGAGGACGCCAATCAACCTTGACCTCCAATGGGTATTAATTGATAACCAAACt                                            >  2:116383/1‑251 (MQ=255)                                                                                                                                                                                                                        aTAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCg                                  >  1:132081/1‑251 (MQ=255)                                                                                                                                                                                                                                           ataGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCACTGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAAc               >  1:262495/1‑251 (MQ=255)                                                                                                                                                                                                                                           ataGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAAc               <  1:114991/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cAAATCCAGTTATCATCATTGGTTGTGCTTCATCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCTCTAAGCACTCTGATTAATAATAAGAGTACGCCAATCCACCTTGACCTCCAATGGGTATGAATTTATAGCCAAACTGCAGTTTGTGTGACACCAGAGCAATTAACAttatta        <  1:182509/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aaaTCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAAt                                                                                                                                           <  1:211387/119‑1 (MQ=255)                                                                                                                                                                                                                                                   aaaTCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTAAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAAt                                                                                                                                           >  2:211387/1‑119 (MQ=255)                                                                                                                                                                                                                                                        cAGTTATCAACATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAAACGAATCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCTCCTTGACCTCCAATGGGTATTAATTGATAACCAATCTGCAGTTTGCGTGACACCAGAGCAATTAACATTATTAGGAttt  <  2:262495/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                          GGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACATTATTAGGATTT  >  NC_000913/3187178‑3187675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.